Ribosomal internal transcribed spacer size variation correlated with RAPD-PCR pattern polymorphisms in the entomopathogenic fungus Erynia neoaphidis and some closely related species

1997 ◽  
Vol 101 (5) ◽  
pp. 573-579 ◽  
Author(s):  
E. Rohel ◽  
Y. Couteaudier ◽  
B. Papierok ◽  
N. Cavelier ◽  
C.A. Dedryver
2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Congzhao Fan ◽  
Xiaojin Li ◽  
Jun Zhu ◽  
Jingyuan Song ◽  
Hui Yao

The medicinal plantFerulahas been widely used in Asian medicine, especially in Uyghur medicine in Xinjiang, China. Given that various substitutes and closely related species have similar morphological characteristics,Ferulais difficult to distinguish based on morphology alone, thereby causing confusion and threatening the safe use ofFerula. In this study, internal transcribed spacer 2 (ITS2) sequences were analyzed and assessed for the accurate identification of two salableFerulaspecies (Ferula sinkiangensisandFerula fukangensis) and eight substitutes or closely related species. Results showed that the sequence length of ITS2 ranged from 451 bp to 45 bp, whereas guanine and cytosine contents (GC) were from 53.6% to 56.2%. A total of 77 variation sites were detected, including 63 base mutations and 14 insertion/deletion mutations. The ITS2 sequence correctly identified 100% of the samples at the species level using the basic local alignment search tool 1 and nearest-distance method. Furthermore, neighbor-joining tree successfully identified the genuine plantsF. sinkiangensisandF. fukangensisfrom their succedaneum and closely related species. These results indicated that ITS2 sequence could be used as a valuable barcode to distinguish Uyghur medicineFerulafrom counterfeits and closely related species. This study may broaden DNA barcoding application in the Uyghur medicinal plant field.


Phytotaxa ◽  
2017 ◽  
Vol 331 (2) ◽  
pp. 273 ◽  
Author(s):  
ARUN KUMAR DUTTA ◽  
SUDESHNA NANDI ◽  
ENTAJ TARAFDER ◽  
RIMPA SIKDER ◽  
ANIRBAN ROY ◽  
...  

A new species of Trogia (Marasmiaceae, Basidiomycota) is described from West Bengal, India. Analysis of the molecular sequence (nuclear ribosomal internal transcribed spacer region) data suggests that T. benghalesis is phylogenetically distinct from its closely related species. Comprehensive description based on macro- and microscopic characters, photographs and comparisons with morphologically similar and phylogenetically related species are provided.


Phytotaxa ◽  
2016 ◽  
Vol 280 (2) ◽  
pp. 116 ◽  
Author(s):  
HUILI LI ◽  
XUELAN MA ◽  
PETER E. MORTIMER ◽  
SAMANTHA C. KARUNARATHNA ◽  
JIANCHU XU ◽  
...  

Four specimens of Phallus were collected during surveys in a Pinus armandii forest in Baoshan, Yunnan Province, China. Macro- and micro-characteristics, together with Internal Transcribed Spacer (ITS) sequence data, showed that the four specimens belong to a new species, here named Phallus haitangensis. The ITS phylogenetic analyses, morphological descriptions, color photographs, and line drawings are provided, and compared with closely related species in the genus.


Phytotaxa ◽  
2015 ◽  
Vol 202 (2) ◽  
pp. 94 ◽  
Author(s):  
Yang Kun Li ◽  
Xin Zhang ◽  
Ye Yuan ◽  
Zheng Cao ◽  
Jun Feng Liang

Russula subrutilans sp. nov., a new species of Russula is described from southern China. It is unique for having buff pink to light congo-pink pileus, distant ventricose to subventricose lamellae with rare lamellulae, globose to broadly ellipsoid spores with bluntly conical warts forming a partial reticulum, and narrowly clavate to clavate cheilocystidia and pleurocysitidia with variable tips. Phylogenetic relationships among the new species and other closely related species in the genus are inferred based on the internal transcribed spacer (ITS) region.


Phytotaxa ◽  
2015 ◽  
Vol 226 (1) ◽  
pp. 1 ◽  
Author(s):  
Passorn Wonnapinij ◽  
Ajaraporn Sriboonlert

Phylogenetic relationships among species of Bulbophyllum sect. Trias and related taxa in Bulbophyllum has not been previously studied due to scarcity of appropriate samples. In this study, we aimed to assess the relationship of these taxa and investigate interspecific relationships of these orchids using DNA sequences from two plastid genes, rbcL and matK, and one nuclear region, nuclear ribosomal internal transcribed spacer, nrITS. Our results showed that all Bulbophyllum sect. Trias species were embedded among other groups of Bulbophyllum, supporting the already published transfer of Trias to synonymy of Bulbophyllum. All trees revealed that the Indochinese sect. Trias form a monophyletic group that could be divided into three groups coincident with their vegetative characters. This study further shows that nrITS sequences can be sufficient for inferring phylogenetic relationship among Trias species, although this marker and the combination of this plus the plastid genes are not able to distinguish the differences between some closely related species.


Phytotaxa ◽  
2020 ◽  
Vol 458 (4) ◽  
pp. 281-293
Author(s):  
RAGHVENDRA SINGH ◽  
SANJEET KUMAR VERMA ◽  
SANJAY YADAV ◽  
PUJA BHOJAK ◽  
SHAMBHU KUMAR

Pseudocercospora hamiltoniani, a new asexual foliicolous hyphomycetous fungus was discovered on living leaves of Euonymus hamiltonianus (Celastraceae) is described and illustrated. This species is compared with closely related species of Pseudocercospora and other cercosporoid forms reported on the same host genus. The phylogeny of this species has been inferred from internal transcribed spacer (ITS). Phylogenetically, Ps. hamiltoniani differ from closely related Ps. ranjita in having smooth to verruculose mycelium; mostly solitary, highly septate, smooth to verruculose, longer and thicker conidiophores and highly septate with oblique septation, presence of rounded cells, smooth to verruculose, longer and thicker conidia.


2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Tsuyoshi Ito ◽  
Takeshi Nishimura ◽  
Masanaru Takai

Interpreting evolutionary history of macaque monkeys from fossil evidence is difficult, because their evolutionary fluctuations in body size might have removed or formed important morphological features differently in each lineage. We employed geometric morphometrics to explore allometric trajectories of craniofacial shape in two closely related species, Macaca fascicularis and M. fuscata. These two species exhibit a single shared allometric trajectory in superoinferior deflection of the anterior face, indicating that the differences in this feature can be explained by size variation. In contrast, two parallel trajectories are demonstrated in craniofacial protrusion, indicating that even if they are comparable in size, M. fuscata has a higher and shorter face than M. fascicularis. The degree of facial protrusion is most likely a critical feature for phyletic evaluation in the fascicularis group. Such analyses in various macaques would help to resolve controversies regarding phyletic interpretations of fossil macaques.


Sign in / Sign up

Export Citation Format

Share Document