Use of an rRNA Internal Transcribed Spacer Region To Distinguish Phylogenetically Closely Related Species of the Genera Zygosaccharomyces and Torulaspora

A new species of Trogia (Marasmiaceae, Basidiomycota) is described from West Bengal, India. Analysis of the molecular sequence (nuclear ribosomal internal transcribed spacer region) data suggests that T. benghalesis is phylogenetically distinct from its closely related species. Comprehensive description based on macro- and microscopic characters, photographs and comparisons with morphologically similar and phylogenetically related species are provided.

Download Full-text

Endangered Uyghur Medicinal PlantFerulaIdentification through the Second Internal Transcribed Spacer

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/479879 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Congzhao Fan ◽

Xiaojin Li ◽

Jun Zhu ◽

Jingyuan Song ◽

Hui Yao

Keyword(s):

Medicinal Plant ◽

Related Species ◽

Internal Transcribed Spacer ◽

Morphological Characteristics ◽

Its2 Sequence ◽

Sequence Length ◽

Local Alignment ◽

Accurate Identification ◽

Distance Method ◽

Closely Related Species

The medicinal plantFerulahas been widely used in Asian medicine, especially in Uyghur medicine in Xinjiang, China. Given that various substitutes and closely related species have similar morphological characteristics,Ferulais difficult to distinguish based on morphology alone, thereby causing confusion and threatening the safe use ofFerula. In this study, internal transcribed spacer 2 (ITS2) sequences were analyzed and assessed for the accurate identification of two salableFerulaspecies (Ferula sinkiangensisandFerula fukangensis) and eight substitutes or closely related species. Results showed that the sequence length of ITS2 ranged from 451 bp to 45 bp, whereas guanine and cytosine contents (GC) were from 53.6% to 56.2%. A total of 77 variation sites were detected, including 63 base mutations and 14 insertion/deletion mutations. The ITS2 sequence correctly identified 100% of the samples at the species level using the basic local alignment search tool 1 and nearest-distance method. Furthermore, neighbor-joining tree successfully identified the genuine plantsF. sinkiangensisandF. fukangensisfrom their succedaneum and closely related species. These results indicated that ITS2 sequence could be used as a valuable barcode to distinguish Uyghur medicineFerulafrom counterfeits and closely related species. This study may broaden DNA barcoding application in the Uyghur medicinal plant field.

Download Full-text

Choiromyces cerebriformis (Tuberaceae), a new hypogeous species from Yunnan, China

Phytotaxa ◽

10.11646/phytotaxa.482.3.3 ◽

2021 ◽

Vol 482 (3) ◽

pp. 251-260

Author(s):

TIAN-JUN YUAN ◽

OLIVIER RASPÉ ◽

YONG-JUN LI ◽

LI WANG ◽

KAI-MEI SU ◽

...

Keyword(s):

Related Species ◽

Internal Transcribed Spacer ◽

Yunnan Province ◽

Genetic Distances ◽

Morphological Evidence ◽

Similar Species ◽

Sequence Analyses ◽

Internal Transcribed Spacer Region ◽

Morphological Differences ◽

Light Orange

A new hypogeous species, Choiromyces cerebriformis sp. nov. is described and illustrated from Yunnan province, China. Both morphological evidence and sequence analyses of the internal transcribed spacer region (ITS1-5.8S-ITS2) support the species as new to science. C. cerebriformis differs from other Choiromyces species in having ascomata with larger lobes and light orange-brown gleba, and globose ascospores with short stick-like sparse spines ornamentation. Morphological differences and genetic distances with the similar species C. helanshanensis and C. alveolatus are discussed. A phenotypic key including related species is provided.

Download Full-text

Phallus haitangensis, a new species of stinkhorn from Yunnan Province, China

Phytotaxa ◽

10.11646/phytotaxa.280.2.2 ◽

2016 ◽

Vol 280 (2) ◽

pp. 116 ◽

Cited By ~ 1

Author(s):

HUILI LI ◽

XUELAN MA ◽

PETER E. MORTIMER ◽

SAMANTHA C. KARUNARATHNA ◽

JIANCHU XU ◽

...

Keyword(s):

New Species ◽

Related Species ◽

Internal Transcribed Spacer ◽

Yunnan Province ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Its Sequence ◽

Closely Related Species ◽

Line Drawings ◽

A New Species

Four specimens of Phallus were collected during surveys in a Pinus armandii forest in Baoshan, Yunnan Province, China. Macro- and micro-characteristics, together with Internal Transcribed Spacer (ITS) sequence data, showed that the four specimens belong to a new species, here named Phallus haitangensis. The ITS phylogenetic analyses, morphological descriptions, color photographs, and line drawings are provided, and compared with closely related species in the genus.

Download Full-text

Morphological and molecular evidence for a new species of Russula (Russulaceae) from southern China

Phytotaxa ◽

10.11646/phytotaxa.202.2.2 ◽

2015 ◽

Vol 202 (2) ◽

pp. 94 ◽

Cited By ~ 8

Author(s):

Yang Kun Li ◽

Xin Zhang ◽

Ye Yuan ◽

Zheng Cao ◽

Jun Feng Liang

Keyword(s):

New Species ◽

Related Species ◽

Phylogenetic Relationships ◽

Internal Transcribed Spacer ◽

Southern China ◽

Its Region ◽

Molecular Evidence ◽

Closely Related Species ◽

A New Species

Russula subrutilans sp. nov., a new species of Russula is described from southern China. It is unique for having buff pink to light congo-pink pileus, distant ventricose to subventricose lamellae with rare lamellulae, globose to broadly ellipsoid spores with bluntly conical warts forming a partial reticulum, and narrowly clavate to clavate cheilocystidia and pleurocysitidia with variable tips. Phylogenetic relationships among the new species and other closely related species in the genus are inferred based on the internal transcribed spacer (ITS) region.

Download Full-text

Ribosomal internal transcribed spacer size variation correlated with RAPD-PCR pattern polymorphisms in the entomopathogenic fungus Erynia neoaphidis and some closely related species

Mycological Research ◽

10.1017/s0953756296002961 ◽

1997 ◽

Vol 101 (5) ◽

pp. 573-579 ◽

Cited By ~ 28

Author(s):

E. Rohel ◽

Y. Couteaudier ◽

B. Papierok ◽

N. Cavelier ◽

C.A. Dedryver

Keyword(s):

Related Species ◽

Entomopathogenic Fungus ◽

Internal Transcribed Spacer ◽

Size Variation ◽

Closely Related Species ◽

Erynia Neoaphidis ◽

Rapd Pcr

Download Full-text

A new species of Russula (Russulales) from Eastern Himalaya, India

Phytotaxa ◽

10.11646/phytotaxa.234.3.6 ◽

2015 ◽

Vol 234 (3) ◽

pp. 255 ◽

Cited By ~ 4

Author(s):

Soumitra Paloi ◽

Arun Kumar Dutta ◽

Krishnendu Acharya

Keyword(s):

New Species ◽

Related Species ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Eastern Himalaya ◽

Present Species ◽

Internal Transcribed Spacer Region ◽

Comprehensive Description ◽

Region Sequence ◽

A New Species

A new species of Russula (Russulaceae, Basidiomycota) is described from Darjeeling Hill, Eastern Himalaya, India. Morphological and molecular (nuclear ribosomal internal transcribed spacer region) sequence data assign the present species to the subgen. Incrustatula Romagn., sect. Lilaceinae (Melzer & Zvára) Konrad & Joss. and subsect. Lilaceinae Melzer & Zvára. Comprehensive description, photographs and comparisons with morphologically similar and phylogenetically related species are provided.

Download Full-text

Molecular phylogenetics of species of Bulbophyllum sect. Trias (Orchidaceae; Epidendroideae; Malaxidae) based on nrITS and plastid rbcL and matK

Phytotaxa ◽

10.11646/phytotaxa.226.1.1 ◽

2015 ◽

Vol 226 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

Passorn Wonnapinij ◽

Ajaraporn Sriboonlert

Keyword(s):

Dna Sequences ◽

Related Species ◽

Phylogenetic Relationships ◽

Internal Transcribed Spacer ◽

Molecular Phylogenetics ◽

Nuclear Region ◽

Closely Related Species ◽

Vegetative Characters ◽

Relationship Of ◽

The Relationship

Phylogenetic relationships among species of Bulbophyllum sect. Trias and related taxa in Bulbophyllum has not been previously studied due to scarcity of appropriate samples. In this study, we aimed to assess the relationship of these taxa and investigate interspecific relationships of these orchids using DNA sequences from two plastid genes, rbcL and matK, and one nuclear region, nuclear ribosomal internal transcribed spacer, nrITS. Our results showed that all Bulbophyllum sect. Trias species were embedded among other groups of Bulbophyllum, supporting the already published transfer of Trias to synonymy of Bulbophyllum. All trees revealed that the Indochinese sect. Trias form a monophyletic group that could be divided into three groups coincident with their vegetative characters. This study further shows that nrITS sequences can be sufficient for inferring phylogenetic relationship among Trias species, although this marker and the combination of this plus the plastid genes are not able to distinguish the differences between some closely related species.

Download Full-text

Three new species of Entoloma subgenus Nolanea from India based on morphology and molecular phylogeny

Phytotaxa ◽

10.11646/phytotaxa.286.4.2 ◽

2016 ◽

Vol 286 (4) ◽

pp. 232 ◽

Cited By ~ 1

Author(s):

K. N.ANIL RAJ ◽

PATINJAREVEETTIL MANIMOHAN

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Maximum Likelihood ◽

Molecular Phylogeny ◽

Related Species ◽

Internal Transcribed Spacer ◽

Internal Transcribed Spacer Region ◽

Three New Species ◽

Kerala State

Three new species of Entoloma belonging to the subgenus Nolanea, E. brunneoumbonatum, E. luteodiscum and E. brunneoapplanatum, are described from Kerala State, India based on morphology and molecular phylogeny. Comprehensive descriptions, photographs, and comparisons with phenetically similar and phylogenetically related species are discussed. The nuclear ribosomal internal transcribed spacer region (ITS) of the three species was sequenced and analyzed. The ITS-based phylogenetic analysis, performed using Maximum Likelihood (ML) method, supported both the novelty of these species and their infrageneric placement within the subgenus Nolanea. A key to the ten species of Entoloma subgenus Nolanea known from India is also provided.

Download Full-text

Marasmiellus griseobrunneus sp. nov. (Omphalotaceae) from Kerala State, India

Phytotaxa ◽

10.11646/phytotaxa.416.1.3 ◽

2019 ◽

Vol 416 (1) ◽

pp. 25-33

Author(s):

SHAHINA A. SHARAFUDHEEN ◽

PATINJAREVEETTIL MANIMOHAN

Keyword(s):

New Species ◽

Maximum Likelihood ◽

Molecular Phylogeny ◽

Ribosomal Dna ◽

Related Species ◽

Internal Transcribed Spacer ◽

Nuclear Ribosomal Dna ◽

Internal Transcribed Spacer Region ◽

Kerala State

Marasmiellus griseobrunneus sp. nov. is described from Kerala State, India, on the basis of both morphology and molecular phylogeny. This species is characterized by a grayish brown, sulcate-striate pileus with a fimbriate margin, weakly to strongly intervenose lamellae, pip-shaped to short lanceolate or lacrymoid basidiospores, versiform cheilo- and pleurocystidia, subregular hymenophoral trama, and the presence of appressed or ascending setae on the pileipellis and stipitipellis. A detailed description, comparison with both morphologically and phylogenetically related species, photographs of both the basidiocarps and the microscopic structures, and a Maximum Likelihood phylogram, based on the sequence of the Internal Transcribed Spacer region of the nuclear ribosomal DNA, depicting the placement of the new species are provided.

Download Full-text