scholarly journals Twenty-four-hour urinary water-soluble vitamin levels correlate with their intakes in free-living Japanese schoolchildren

2010 ◽  
Vol 14 (2) ◽  
pp. 327-333 ◽  
Author(s):  
Tomiko Tsuji ◽  
Tsutomu Fukuwatari ◽  
Satoshi Sasaki ◽  
Katsumi Shibata
Keyword(s):  
Elementary School ◽  
Pantothenic Acid ◽  
Water Soluble ◽  
Vitamin B ◽  
Food Record ◽  
Free Living ◽  
Water Soluble Vitamin ◽  
Potential Biomarker ◽  
Urinary Levels ◽  
Weighed Food Record

AbstractObjectiveTo examine the association between 24 h urinary water-soluble vitamin levels and their intakes in free-living Japanese schoolchildren.DesignAll foods consumed for four consecutive days were recorded accurately by a weighed food record. A single 24 h urine sample was collected on the fourth day, and the urinary levels of water-soluble vitamins were measured.SettingAn elementary school in Inazawa City, Japan.SubjectsA total of 114 healthy, free-living, Japanese elementary-school children aged 10–12 years.ResultsThe urinary level of each water-soluble vitamin was correlated positively to its mean intake in the past 2–4 d (vitamin B1: r = 0·42, P < 0·001; vitamin B2: r = 0·43, P < 0·001; vitamin B6: r = 0·49, P < 0·001; niacin: r = 0·32, P < 0·001; niacin equivalents: r = 0·32, P < 0·001; pantothenic acid: r = 0·32, P < 0·001; folic acid: r = 0·27, P < 0·01; vitamin C: r = 0·39, P < 0.001), except for vitamin B12 (r = 0·10, P = NS). Estimated mean intakes of water-soluble vitamins calculated using urinary levels and recovery rates were 97–102 % of their 3 d mean intake, except for vitamin B12 (79 %).ConclusionsThe results show that urinary levels of water-soluble vitamins, except for vitamin B12, reflected their recent intakes in free-living Japanese schoolchildren and could be used as a potential biomarker to estimate mean vitamin intake.

scholarly journals Concentrations of Water-Soluble Vitamins in Blood and Urinary Excretion in Patients with Diabetes Mellitus

2016 ◽  
Vol 9 ◽  
pp. NMI.S40595 ◽  
Author(s):  
Hiromi Iwakawa ◽  
Yasuyuki Nakamura ◽  
Tomiho Fukui ◽  
Tsutomu Fukuwatari ◽  
Satoshi Ugi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Urinary Excretion ◽  
Water Soluble ◽  
Confounding Factors ◽  
Linear Regression Models ◽  
Food Record ◽  
Patients With Diabetes ◽  
Weighed Food Record ◽  
Healthy Control ◽  
Renal Clearances

We examined the concentrations of water-soluble vitamins in blood and urinary excretion of 22 patients with type 2 diabetes mellitus (type 2DM) and 20 healthy control participants. Macronutrient and vitamin intakes of type 2DM subjects were measured using a weighed food record method. Control participants consumed a semipurified diet for eight days. Multiple linear regression models were used to determine whether significant differences existed in vitamin concentrations in blood independent of age, sex, and other confounding factors. Concentrations of vitamins B2, B6, C, niacin, and folate in blood were significantly lower in type 2DM subjects than in controls, independent of confounding factors. Renal clearances of vitamins B6, C, niacin, and folate were significantly higher in type 2DM subjects than in controls. In conclusion, concentrations of vitamins B2, B6, C, niacin, and folate in blood were significantly lower in type 2DM subjects than in controls, independent of confounding factors; based on the evidence of increased urinary clearance of these vitamins, the lower levels were likely due to impaired reabsorption processes.

scholarly journals Organometallic cobalamin anticancer derivatives for targeted prodrug delivery via transcobalamin-mediated uptake

2017 ◽  
Vol 46 (7) ◽  
pp. 2159-2164 ◽  
Author(s):  
Jeremie Rossier ◽  
Daniel Hauser ◽  
Emmanuel Kottelat ◽  
Barbara Rothen-Rutishauser ◽  
Fabio Zobi
Keyword(s):  
Metal Complexes ◽  
Water Soluble ◽  
Vitamin B ◽  
Water Soluble Vitamin ◽  
Cobalt Center

We report the synthesis and study of new water-soluble vitamin B12 prodrugs bearing metal complexes at the β-upper side of the cobalt center for targeted prodrug delivery.

Pyridoxine uptake by colonocytes: a specific and regulated carrier-mediated process

2008 ◽  
Vol 294 (5) ◽  
pp. C1192-C1197 ◽  
Author(s):  
Zainab M. Said ◽  
Veedamali S. Subramanian ◽  
Nosratola D. Vaziri ◽  
Hamid M. Said
Keyword(s):  
Adult Mouse ◽  
Initial Rate ◽  
Apical Membrane ◽  
Membrane Vesicles ◽  
Water Soluble ◽  
Vitamin B ◽  
Cellular Functions ◽  
Normal Microflora ◽  
Water Soluble Vitamin ◽  
First Time

The water-soluble vitamin B6 (pyridoxine) is important for normal cellular functions, growth, and development. The vitamin is obtained from two exogenous sources: a dietary source, which is absorbed in the small intestine, and a bacterial source, where the vitamin is synthesized in significant quantities by the normal microflora of the large intestine. Evidence exists to suggest the bioavailability of the latter source of the vitamin, but nothing is known about the mechanism involved and its regulation. In this study, we addressed these issues using young adult mouse colonic epithelial (YAMC) cells and human colonic apical membrane vesicles (AMV) as models and using [3H]pyridoxine as the uptake substrate. The results showed the initial rate of [3H]pyridoxine uptake by YAMC cells to be 1) energy- and temperature- (but not Na-) dependent and to occur without metabolic alteration in the transported substrate; 2) saturable as a function of concentration with an apparent Km and Vmax of 2.1 ± 0.5 μM and 53.4 ± 4.3 pmol·mg protein−1·3 min−1, respectively; 3) cis-inhibited by unlabeled pyridoxine and its structural analogs, but not by the unrelated compounds theophylline, penicillamine, and isoniazid; 4) trans-stimulated by unlabeled pyridoxine; 5) amiloride sensitive; and 6) regulated by extracellular and intracellular factors. Uptake of pyridoxine by native human colonic AMV was also found to involve a carrier-mediated process. These studies demonstrate, for the first time, the functional existence of a specific and regulatable carrier-mediated process for pyridoxine uptake by mammalian colonocytes.

scholarly journals Conditional knockout of the Slc5a6 gene in mouse intestine impairs biotin absorption

2013 ◽  
Vol 304 (1) ◽  
pp. G64-G71 ◽  
Author(s):  
Abhisek Ghosal ◽  
Nils Lambrecht ◽  
Sandeep B. Subramanya ◽  
Rubina Kapadia ◽  
Hamid M. Said
Keyword(s):  
Pantothenic Acid ◽  
Microscopic Analysis ◽  
Water Soluble ◽  
Conditional Knockout ◽  
Relative Contribution ◽  
Transport Studies ◽  
Water Soluble Vitamin ◽  
Active Inflammation

The Slc5a6 gene expresses a plasma membrane protein involved in the transport of the water-soluble vitamin biotin; the transporter is commonly referred to as the sodium-dependent multivitamin transporter (SMVT) because it also transports pantothenic acid and lipoic acid. The relative contribution of the SMVT system toward carrier-mediated biotin uptake in the native intestine in vivo has not been established. We used a Cre/lox technology to generate an intestine-specific (conditional) SMVT knockout (KO) mouse model to address this issue. The KO mice exhibited absence of expression of SMVT in the intestine compared with sex-matched littermates as well as the expected normal SMVT expression in other tissues. About two-thirds of the KO mice died prematurely between the age of 6 and 10 wk. Growth retardation, decreased bone density, decreased bone length, and decreased biotin status were observed in the KO mice. Microscopic analysis showed histological abnormalities in the small bowel (shortened villi, dysplasia) and cecum (chronic active inflammation, dysplasia) of the KO mice. In vivo (and in vitro) transport studies showed complete inhibition in carrier-mediated biotin uptake in the intestine of the KO mice compared with their control littermates. These studies provide the first in vivo confirmation in native intestine that SMVT is solely responsible for intestinal biotin uptake. These studies also provide evidence for a casual association between SMVT function and normal intestinal health.

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