scholarly journals Improved Biological Tissue Preparation Procedure for Scanning Electron Microscopic Imaging

2018 ◽  
Vol 24 (S1) ◽  
pp. 1308-1309
Author(s):  
Habeeb Alsudani ◽  
Soumitra Ghoshroy ◽  
Joseph Quattro ◽  
Matthew Greenwold ◽  
Roger Sawyer
Keyword(s):  
Biological Tissue ◽  
Preparation Procedure ◽  
Tissue Preparation ◽  
Electron Microscopic ◽  
Microscopic Imaging ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

scholarly journals All That Glitters is not Gold: Approaches to Labeling for EM

2002 ◽  
Vol 10 (3) ◽  
pp. 24-26
Author(s):  
R.M. Albrecht ◽  
D.A. Meyer
Keyword(s):  
Heavy Metals ◽  
Spatial Resolution ◽  
Imaging Systems ◽  
Electron Microscopic ◽  
Microscopic Imaging ◽  
Virus Particles ◽  
Transmission Electron ◽  
Scanning Electron Microscopic ◽  
Electron Imaging ◽  
Scanning Electron

A large number of natural and synthetic species ranging from virus particles to small polymer beads have been employed over trie years as labels in electron microscopic applications. Perhaps the most useful and versatile of these labels are the colloidal heavy metals. The colloidal labels can be synthesized as round regular spheres in sizes from 1 to 150 nanometers in diameter and thus can be used where molecular and sub-molecular ranges of spatial resolution are required. They are electron “dense” and good emitters of secondary and back-scattered electrons and hence are readily detectable in both scanning electron microscopic imaging systems and transmission electron imaging systems. Larger particles, 10nm and above, can be identified via their shape in force based imaging systems.

Scanning electron microscopic imaging of surface effects in desorption and nano-desorption electrospray ionization

2011 ◽  
Vol 46 (3) ◽  
pp. 256-261 ◽  
Author(s):  
Filip Kaftan ◽  
Olga Kofroňová ◽  
Oldřich Benada ◽  
Karel Lemr ◽  
Vladimír Havlíček ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Desorption Electrospray Ionization ◽  
Surface Effects ◽  
Electron Microscopic ◽  
Microscopic Imaging ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Preparation of Articular Cartilage for Scanning Electron Microscopy

1973 ◽  
Vol 31 ◽  
pp. 436-437
Author(s):  
M. L. Zimny ◽  
I. Redler ◽  
J. Fernandez
Keyword(s):  
Articular Cartilage ◽  
Femoral Condyle ◽  
Tissue Preparation ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Scanning Electron Microscopic ◽  
Electron Microscopic Image ◽  
The Relationship ◽  
Scanning Electron Microscopic Image ◽  
Scanning Electron

The relationship between tissue preparation and a scanning electron microscopic image is still somewhat nebulous. For purposes of answering comments about our observations of articular cartilage, we decided to prepare tissue samples from one area of a femoral condyle, obtained at surgery, in several ways. As a result of this investigation we hoped to find the best way to prepare articular cartilage for maximal visual detail and subsequently standardize our procedures for a comparative study of normal and arthritic cartilage.The samples were fixed as follows: (1) no fixative; (2) 2% glutaraldehyde buffered with cacodylate, pH 7.4; (3) 10% buffered formalin; (4) 0.1% glutaraldehyde in Ringer's; and (5) 1% glutaraldehyde in Ringer's.

Scanning Electron Microscopic Study of the Cell Surface

1969 ◽  
Vol 27 ◽  
pp. 36-37 ◽  
Author(s):  
Toichiro Kuwabara
Keyword(s):  
Tissue Fluid ◽  
Biological Application ◽  
Biological Structure ◽  
Electron Microscopic ◽  
Surface Appearance ◽  
Minute Amount ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
True Structure ◽  
Scanning Electron

Although scanning electron microscopy has a great potential in biological application, there are certain limitations in visualization of the biological structure. Satisfactory techniques to demonstrate natural surfaces of the tissue and the cell have been reported by several investigators. However, it is commonly found that the surface cell membrane is covered with a minute amount of mucin, secretory substance or tissue fluid as physiological, pathological or artefactual condition. These substances give a false surface appearance, especially when the tissue is fixed with strong fixatives. It seems important to remove these coating substances from the surface of the cell for demonstration of the true structure.

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