Multiple genes confer resistance to soybean mosaic virus in the soybean cultivar Hwangkeum

2014 ◽  
Vol 12 (S1) ◽  
pp. S41-S44 ◽  
Author(s):  
Namhee Jeong ◽  
Soon-Chun Jeong
Keyword(s):  
Mosaic Virus ◽  
Recombinant Inbred Lines ◽  
Soybean Mosaic Virus ◽  
Durable Resistance ◽  
Wild Soybean ◽  
Soybean Cultivar ◽  
Breeding Programmes ◽  
Cultivated Species ◽  
Cultivated Soybean ◽  
Efficient Selection

Recombinant inbred lines (RILs) generated from a cross between a cultivated species and its wild progenitor species serve as important germplasm for introgressing valuable genes from a wild species to a cultivated species. During this breeding process, it is equally important to prevent the loss of agronomically important genes in the cultivated species. In an effort to establish an efficient selection system for the single Rsv1 gene conferring durable resistance to soybean mosaic virus (SMV) in the soybean cultivar Hwangkeum (also known as Suweon 97), which is a parent of a RIL population from Hwangkeum (cultivated soybean) × IT182932 (wild soybean), in the present study, we unexpectedly identified an additional necrosis-conditioning gene unmasked by a recombination in the middle of the Rsv1-containing nucleotide-binding leucine-rich repeat gene cluster region and the Rsv3 gene in Hwangkeum. Thus, Hwangkeum contains at least three SMV resistance genes consisting of two tightly linked genes at the Rsv1 locus and the Rsv3 locus. The results of this study provide additional important information for use of the Hwangkeum genome in soybean breeding programmes.

scholarly journals Precise Exchange of the Helper-Component Proteinase Cistron Between Soybean mosaic virus and Clover yellow vein virus: Impact on Virus Viability and Host Range Specificity

2020 ◽  
Vol 110 (1) ◽  
pp. 206-214 ◽  
Author(s):  
Y. Wang ◽  
W. Xu ◽  
J. Abe ◽  
K. S. Nakahara ◽  
M. R. Hajimorad
Keyword(s):  
Host Range ◽  
Mosaic Virus ◽  
Broad Bean ◽  
Soybean Mosaic Virus ◽  
Wild Soybean ◽  
Systemic Infection ◽  
Yellow Vein ◽  
Clover Yellow Vein Virus ◽  
Helper Component Proteinase ◽  
Cultivated Soybean

Soybean mosaic virus and Clover yellow vein virus are two definite species of the genus Potyvirus within the family Potyviridae. Soybean mosaic virus-N (SMV-N) is well adapted to cultivated soybean (Glycine max) genotypes and wild soybean (G. soja), whereas it remains undetectable in inoculated broad bean (Vicia faba). In contrast, clover yellow vein virus No. 30 (ClYVV-No. 30) is capable of systemic infection in broad bean and wild soybean; however, it infects cultivated soybean genotypes only locally. In this study, SMV-N was shown to also infect broad bean locally; hence, broad bean is a host for SMV-N. Based on these observations, it was hypothesized that lack of systemic infection by SMV-N in broad bean and by ClYVV-No. 30 in cultivated soybean is attributable to the incompatibility of multifunctional helper-component proteinase (HC-Pro) in these hosts. The logic of selecting the HC-Pro cistron as a target is based on its established function in systemic movement and being a relevant factor in host range specificity of potyviruses. To test this hypothesis, chimeras were constructed with precise exchanges of HC-Pro cistrons between SMV-N and ClYVV-No. 30. Upon inoculation, both chimeras were viable in infection, but host range specificity of the recombinant viruses did not differ from those of the parental viruses. These observations suggest that (i) HC-Pro cistrons from SMV-N and ClYVV-No. 30 are functionally compatible in infection despite 55.6 and 48.9% nucleotide and amino acid sequence identity, respectively, and (ii) HC-Pro cistrons from SMV-N and ClYVV-No. 30 are not the determinants of host specificity on cultivated soybean or broad beans, respectively.

scholarly journals RNAi-mediated Soybean mosaic virus (SMV) resistance of a Korean Soybean cultivar

2016 ◽  
Vol 10 (5) ◽  
pp. 257-267 ◽  
Author(s):  
Hye Jeong Kim ◽  
Mi-Jin Kim ◽  
Jung Hun Pak ◽  
Hyun Hee Im ◽  
Dong Hee Lee ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Soybean Cultivar

scholarly journals An Avirulent Strain of Soybean Mosaic Virus Reverses the Defensive Effect of Abscisic Acid in a Susceptible Soybean Cultivar

Viruses ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 879 ◽  
Author(s):  
Mazen Alazem ◽  
Kristin Widyasari ◽  
Kook-Hyung Kim
Keyword(s):  
Abscisic Acid ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Defense Mechanisms ◽  
Virulent Strain ◽  
Soybean Mosaic Virus ◽  
Resistance Mechanisms ◽  
Soybean Cultivar ◽  
Avirulent Strain ◽  
Rapid Induction

In soybean cultivar L29, the Rsv3 gene is responsible for extreme resistance (ER) against the soybean mosaic virus avirulent strain G5H, but is ineffective against the virulent strain G7H. Part of this ER is attributed to the rapid increase in abscisic acid (ABA) and callose, and to the rapid induction of several genes in the RNA-silencing pathway. Whether these two defense mechanisms are correlated or separated in the ER is unknown. Here, we found that ABA treatment of L29 plants increased the expression of several antiviral RNA-silencing genes as well as the PP2C3a gene, which was previously shown to increase callose accumulation; as a consequence, ABA increased the resistance of L29 plants to G7H. The effect of ABA treatment on these genes was weaker in the rsv3-null cultivar (Somyungkong) than in L29. Besides, G5H-infection of Somyungkong plants subverted the effect of ABA leading to reduced callose accumulation and decreased expression of several RNA-silencing genes, which resulted in increased susceptibility to G5H infection. ABA treatment, however, still induced some resistance to G7H in Somyungkong, but only AGO7b was significantly induced. Our data suggest that Rsv3 modulates the effect of ABA on these two resistance mechanisms, i.e., callose accumulation and the antiviral RNA-silencing pathway, and that in the absence of Rsv3, some strains can reverse the effect of ABA and thereby facilitate their replication and spread.

Fine mapping of the Rsv1-h gene in the soybean cultivar Suweon 97 that confers resistance to two Chinese strains of the soybean mosaic virus

2016 ◽  
Vol 129 (11) ◽  
pp. 2227-2236 ◽  
Author(s):  
Fang-Fang Ma ◽  
Xiao-Yi Wu ◽  
Yun-Xia Chen ◽  
Ying-Na Liu ◽  
Zhu-Qing Shao ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Fine Mapping ◽  
Soybean Mosaic Virus ◽  
Soybean Cultivar ◽  
H Gene

Genetic diversity and population structure of wild soybean (Glycine soja Sieb. and Zucc.) accessions in Korea

2014 ◽  
Vol 12 (S1) ◽  
pp. S45-S48 ◽  
Author(s):  
Kil Hyun Kim ◽  
Seukki Lee ◽  
Min-Jung Seo ◽  
Gi-An Lee ◽  
Kyung-Ho Ma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Glycine Soja ◽  
Crop Improvement ◽  
Wild Soybean ◽  
Background Information ◽  
Ssr Loci ◽  
Breeding Programmes ◽  
Western Side ◽  
Cultivated Soybean

Genetic variation in wild soybean (Glycine soja Sieb. and Zucc.) is a valuable resource for crop improvement efforts. Soybean is believed to have originated from China, Korea, and Japan, but little is known about the diversity or evolution of Korean wild soybean. Therefore, in this study, we evaluated the genetic diversity and population structure of 733 G. soja accessions collected in Korea using 21 simple sequence repeat (SSR) markers. The SSR loci produced 539 alleles (25.7 per locus) with a mean genetic diversity of 0.882 in these accessions. Rare alleles, those with a frequency of less than 5%, represented 75% of the total number. This collection was divided into two populations based on the principal coordinate analysis. Accessions from population 1 were distributed throughout the country, whereas most of the accessions from population 2 were distributed on the western side of the Taebaek and Sobaek mountains. The Korean G. soja collection evaluated in this study should provide useful background information for allele mining approach and breeding programmes to introgress alleles into the cultivated soybean (G. max (L). Merr.) from wild soybean.

Identification of haplotypes at the Rsv4 genomic region in soybean associated with durable resistance to soybean mosaic virus

2015 ◽  
Vol 129 (3) ◽  
pp. 453-468 ◽  
Author(s):  
Daniel C. Ilut ◽  
Alexander E. Lipka ◽  
Namhee Jeong ◽  
Dong Nyuk Bae ◽  
Dong Hyun Kim ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Durable Resistance ◽  
Genomic Region
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