Mechanism of leaf rust resistance in wheat wild relatives, Triticum monococcum and T. boeoticum

2021 ◽  
pp. 1-8
Author(s):  
Amandeep K. Riar ◽  
Parveen Chhuneja ◽  
Beat Keller ◽  
Kuldeep Singh
Keyword(s):  
Leaf Rust ◽  
Laser Scanning ◽  
Rust Resistance ◽  
Resistance Mechanism ◽  
Leaf Rust Resistance ◽  
Wheat Breeding ◽  
Wild Relatives ◽  
Triticum Monococcum ◽  
Laser Scanning Microscopy ◽  
Confocal Laser

Abstract Triticum monococcum L. and T. boeoticum L., diploid wild relatives of bread wheat (T. aestivum L.), possess resistance to leaf rust (also known as brown rust) caused by Puccinia triticina Eriks. Haustorium formation-based resistance mechanisms (i.e. pre-haustorial and post-haustorial resistance) to leaf rust have been studied and reported in various T. monococcum accessions. In the present study, the mechanism of leaf rust resistance in T. monococcum and T. boeoticum accessions was studied using confocal laser scanning microscopy. Components of resistance studied at a histological level against leaf rust pathotypes, a Mexican pathotype (TCB/TD) and a Swiss pathotype (97512-19), indicated different types of resistance mechanism operative in the two accessions. The resistance in T. monococcum ranged from pre-haustorial resistance against 97512-19 to post-haustorial resistance against TCB/TD. The response in T. boeoticum was post-haustorial with necrosis against the two pathotypes. Pre-haustorial resistance observed in T. monococcum could serve as a potential source of durable rust resistance in wheat breeding.

scholarly journals Fine Mapping of the Wheat Leaf Rust Resistance Gene Lr42

2019 ◽  
Vol 20 (10) ◽  
pp. 2445 ◽  
Author(s):  
Harsimardeep S. Gill ◽  
Chunxin Li ◽  
Jagdeep S. Sidhu ◽  
Wenxuan Liu ◽  
Duane Wilson ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Hexaploid Wheat ◽  
Rust Resistance ◽  
Reference Genome ◽  
Aegilops Tauschii ◽  
Leaf Rust Resistance ◽  
Wheat Breeding ◽  
Chromosome 1 ◽  
Adult Plant ◽  
Leaf Rust Resistance Gene

Leaf rust caused by Puccinia triticina Eriks is one of the most problematic diseases of wheat throughout the world. The gene Lr42 confers effective resistance against leaf rust at both seedling and adult plant stages. Previous studies had reported Lr42 to be both recessive and dominant in hexaploid wheat; however, in diploid Aegilops tauschii (TA2450), we found Lr42 to be dominant by studying segregation in two independent F2 and their F2:3 populations. We further fine-mapped Lr42 in hexaploid wheat using a KS93U50/Morocco F5 recombinant inbred line (RIL) population to a 3.7 cM genetic interval flanked by markers TC387992 and WMC432. The 3.7 cM Lr42 region physically corresponds to a 3.16 Mb genomic region on chromosome 1DS based on the Chinese Spring reference genome (RefSeq v.1.1) and a 3.5 Mb genomic interval on chromosome 1 in the Ae. tauschii reference genome. This region includes nine nucleotide-binding domain leucine-rich repeat (NLR) genes in wheat and seven in Ae. tauschii, respectively, and these are the likely candidates for Lr42. Furthermore, we developed two kompetitive allele-specific polymorphism (KASP) markers (SNP113325 and TC387992) flanking Lr42 to facilitate marker-assisted selection for rust resistance in wheat breeding programs.

Incorporation of leaf rust resistance genes into winter wheat genotypes using marker-assisted selection

2007 ◽  
Vol 55 (2) ◽  
pp. 149-156 ◽  
Author(s):  
M. Gál ◽  
G. Vida ◽  
A. Uhrin ◽  
Z. Bedő ◽  
O. Veisz
Keyword(s):  
Winter Wheat ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Breeding ◽  
Wheat Varieties ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

The breeding and cultivation of resistant wheat varieties is an effective way of controlling leaf rust ( Puccinia triticina Eriks.). The use of molecular markers facilitates the incorporation of the major leaf rust resistance genes ( Lr genes) responsible for resistance into new varieties and the pyramiding of these genes. Marker-assisted selection was used to incorporate the Lr genes currently effective in Hungary ( Lr9 , Lr24 , Lr25 , Lr29 ) into winter wheat varieties. The Lr genes were identified using STS, SCAR and RAPD markers closely linked to them. Investigations were made on how these markers could be utilised in plant breeding, and near-isogenic lines resembling the recurrent variety but each containing a different Lr gene were developed to form the initial stock for the pyramiding of resistance genes. The results indicate that the marker-assisted selection technique elaborated for resistance genes Lr24 , Lr25 and Lr29 can be applied simply and effectively in wheat breeding, while the detection of the Lr9 marker is uncertain.

scholarly journals Efficiency of a Seedling Phenotyping Strategy to Support European Wheat Breeding Focusing on Leaf Rust Resistance

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 628
Author(s):  
Ulrike Beukert ◽  
Nina Pfeiffer ◽  
Erhard Ebmeyer ◽  
Valentin Hinterberger ◽  
Stefanie Lueck ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Developmental Stages ◽  
Leaf Rust Resistance ◽  
Wheat Breeding ◽  
Resource Saving ◽  
Detached Leaf ◽  
Controlled Conditions ◽  
Computer Based ◽  
Pearson Correlations

Leaf rust resistance is of high importance for a sustainable European wheat production. The expression of known resistance genes starts at different developmental stages of wheat. Breeding for resistance can be supported by a fast, precise, and resource-saving phenotyping. The examination of detached leaf assays of juvenile plants inoculated under controlled conditions and phenotyped by a robotic- and computer-based, high-throughput system is a promising approach in this respect. Within this study, the validation of the phenotyping workflow was conducted based on a winter wheat set derived from Central Europe and examined at different plant developmental stages. Moderate Pearson correlations of 0.38–0.45 comparing leaf rust resistance of juvenile and adult plants were calculated and may be mainly due to different environmental conditions. Specially, the infection under controlled conditions was limited by the application of a single rust race at only one time point. Our results suggest that the diversification with respect to the applied rust race spectrum is promising to increase the consistency of detached leaf assays and the transferability of its results to the field.

scholarly journals Identification of New Leaf Rust Resistance Loci in Wheat and Wild Relatives by Array-Based SNP Genotyping and Association Genetics

2020 ◽  
Vol 11 ◽  
Author(s):  
Fizza Fatima ◽  
Brent D. McCallum ◽  
Curtis J. Pozniak ◽  
Colin W. Hiebert ◽  
Curt A. McCartney ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wild Relatives ◽  
Snp Genotyping ◽  
Association Genetics

scholarly journals Research Advances in Wheat Breeding and Genetics for Leaf Rust Resistance

2020 ◽  
Vol 52 (3) ◽  
pp. 213-224
Author(s):  
Kyeong-Min Kim ◽  
Seong-Woo Cho ◽  
Chon-Sik Kang ◽  
Kyeong-Hoon Kim ◽  
Chang-Hyun Choi ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Breeding ◽  
Breeding And Genetics

A molecular marker of leaf rust resistance gene Lr37 in wheat

2004 ◽  
Vol 1 (3) ◽  
pp. 139-142 ◽  
Author(s):  
Zhang Li-Rong ◽  
Xu Da-Qing ◽  
Yang Wen-Xiang ◽  
Liu Da-Qun
Keyword(s):  
Molecular Marker ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Polymorphic Marker ◽  
Inter Simple Sequence Repeat ◽  
Wheat Breeding ◽  
Leaf Rust Resistance Gene ◽  
Simple Sequence

AbstractInter-simple sequence repeat (ISSR) analysis was carried out in Thatcher, 20 near-isogenic lines (NILs) containing respectively different genes conferring resistance against wheat leaf rust (Puccinia recondite f.sp. tritici), three materials carrying Lr37 and three materials without Lr37. All of the 100 ISSR primers showed clear amplification products. Two of them amplified the polymorphic DNA bands in the NILs, Thatcher and Lr37/6*Thatcher. The polymorphic bands were named UBC812-1200 and UBC848-700, respectively. The three materials with and without Lr37 were detected in tests using the two primers UBC812 and UBC848. Results also showed that only band UBC812-1200 was amplified in all resistant and absent in all susceptible materials. This suggests that UBC812-1200 marker is linked to the resistance gene Lr37. The genetic linkage of the polymorphic marker with Lr37 was tested using a segregating F2 population (128 plants) derived from a cross between the leaf rust-resistant Lr37/6*Thatcher and the susceptible cultivar Thatcher. The ISSR marker UBC812-1200 showed co-segregation to the Lr37 resistance gene. It could be used in molecular marker-assisted selection in a wheat breeding programme for leaf rust resistance.

The inheritance of leaf and stem rust resistance in Triticum monococcum L.

1998 ◽  
Vol 78 (2) ◽  
pp. 223-226 ◽  
Author(s):  
D. Bai ◽  
D. R. Knott ◽  
J. M. Zale
Keyword(s):  
Leaf Rust ◽  
Stem Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Triticum Monococcum ◽  
Key Words Wheat ◽  
Resistant Accession ◽  
The University ◽  
Moderately Resistant

Resistance to leaf rust (Puccinia recondita f. sp. tritici Rob. ex Desm.) is common in Triticum monococcum L. For example, the 49 accessions in the University of Saskatchewan collection all gave a fleck reaction to leaf rust race CBB. To obtain some indication of whether they all carried the same gene, a set of diallel crosses was made among five of the accessions and three extra crosses were made between two additional accessions and two in the diallel set. The 13 F2 populations involving a total of seven accessions were tested with LR CBB and no segregation for susceptible seedlings occurred. Thus, the seven T. monococcum accessions all carried at least one gene in common. To determine the number of genes involved in leaf rust resistance, four crosses were made between a highly resistant accession, TM157 (IT 0;), and four moderately resistant ones (IT 2−). The F2 populations segregated for two independent dominant genes, one conditioning a fleck reaction and one a type 2 reaction. All seven highly resistant accessions must carry the first gene. Two of the T. monococcum accessions were resistant to stem rust (P. graminisf. sp. tritici Eriks. & Henn.) SR TMH. They proved to carry single genes for resistance, Sr22 in TM65 and Sr35 in TM157. Key words: Wheat, leaf rust, stem rust, inheritance

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