The relationship between DOMD and gas release estimated in vitro using the Reading Pressure Technique system for four maize silages of different maturity

1999 ◽  
Vol 1999 ◽  
pp. 150-150 ◽  
Author(s):  
F.L. Mould ◽  
T. Smith ◽  
E. Owen ◽  
R.H. Phipps
Keyword(s):  
Microbial Biomass ◽  
Biomass Yield ◽  
Gas Production ◽  
Gas Release ◽  
Bicarbonate Buffer ◽  
Fermentation Efficiency ◽  
Using Data ◽  
The Relationship ◽  
Major Criticism

A major criticism of in vitro gas systems is that measurement of nutritionally wasteful products (i.e. CH4 and CO2, either directly from fermentation or indirectly from neutralization of VFA by the bicarbonate buffer) provides little useful information. However, as VFA and gas production are inversely related to microbial biomass yield for any given unit of substrate degraded, it has been suggested (e.g. Getachew et al., 1998) that an estimate of fermentation efficiency or partitioning factor (PF) can be obtained by relating gas production (ml) to the extent of degradation (mg). This study examined the relationship between in vitro DOMD, gas release and PF values using data obtained from an assessment of four maize silages.

scholarly journals The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages

1997 ◽  
Vol 77 (6) ◽  
pp. 911-921 ◽  
Author(s):  
M. Blümmel ◽  
H. Steingaβ ◽  
K. Becker
Keyword(s):  
Microbial Biomass ◽  
Biomass Yield ◽  
Gas Production ◽  
Fatty Acid Production ◽  
Volume Measurements ◽  
N Incorporation ◽  
Highly Correlated ◽  
Gas Volume ◽  
The Relationship

AbstractThe relationship between in vitro gas production, concomitant in vitro apparent and true DM degradability has been examined in forty-two roughages. The partitioning of truly-degraded substrate between gas volume and microbial biomass yield and 15N incorporation into cells was also investigated. The relevance of this partitioning for the regulation of DM intake (DMI) was examined for fifty-four roughages. The results can be summarized as follows. In vitro gas production and in vitro apparent and true degradability are highly correlated (P<0.0001), r being 0.96 and 0.95 respectively. There is an inverse relationship between in vitro gas production and microbial biomass yield (r—0.67, (P<0.0001) and also 15N enrichment (P<0.001)when the variables were related to a given unit of substrate truly degraded. Selecting roughages by in vitro gas production may well be a selection against maximum microbial yield and a combination of in vitro gas volume measurements with a complementary determination of the substrate truly degraded is proposed, to calculate a partitioning factor (PF) reflecting the variation of short-chain fatty acid production per unit substrate degraded. PF is calculated as the ratio, substrate truly degraded: gas produced by it. PF was highly significant (P<0.0001) in DMI prediction when included in stepwise multiple correlations together with in vitro gas volume variables reflecting the extent and rate of gas production; 11 % of the variation in DMI was accounted for by the PF. The total model, including extent and rate of gas production and the PF, accounted for 84 % of the variation in DMI. Roughages producing proportionally less gas per unit substrate truly degraded had higher feed intakes.

Effect of the ratio of maize cob and husk to napier Pakchong 1 silage on nutritive value and in vitro gas production of rumen fluid of Thai native cattle

2017 ◽  
Vol 57 (8) ◽  
pp. 1603 ◽  
Author(s):  
S. Yammuen-art ◽  
P. Somrak ◽  
C. Phatsara
Keyword(s):  
Microbial Biomass ◽  
Nutritive Value ◽  
Biomass Yield ◽  
Rumen Fluid ◽  
Gas Production ◽  
Average Weight ◽  
In Vitro Gas Production ◽  
Native Cattle ◽  
Maize Cob

The present study evaluated the chemical composition and in vitro ruminal digestibility of napier Pakchong 1 silage combined with maize cob and husk in different ratios. The napier Pakchong 1 grass was harvested at 45 days of maturity. The napier Pakchong 1 grass was ensiled with maize cob and husk at ratios of 1:5, 1:10 and 1:15. Three rumen fistulated Thai native cattle (White Lamphun cattle) with an average weight of 154 ± 4.7 kg were used to determine ruminal digestibility by in vitro gas-production technique. Gas production was recorded after incubating for 2, 4, 8, 12, 24, 48, 72 and 96 h. The microbial biomass yield was determined after incubating for 24 h. DM, NDF and ADF of maize cob and husk mixed with napier Pakchong 1 silage declined by increasing the proportion of napier Pakchong 1 grass, while gas production after 4–10 h of incubating maize cob and husk mixed with napier Pakchong 1 grass increased by increasing the proportion of napier Pakchong 1 grass. The metabolisable energy, organic matter digestibility and microbial biomass yield did not differ among the different ratios. The results of the study suggested a recommended ratio of maize cob and husk to napier Pakchong 1 grass of 1:10. The ensiling fermentation increased the proportion of protein in the roughage, which lead to increased in vitro gas production of roughage.

scholarly journals Corrigendum to: Effect of the ratio of maize cob and husk to napier Pakchong 1 silage on nutritive value and in vitro gas production of rumen fluid of Thai native cattle

2019 ◽  
Vol 59 (3) ◽  
pp. 600
Author(s):  
S. Yammuen-art ◽  
P. Somrak ◽  
C. Phatsara
Keyword(s):  
Microbial Biomass ◽  
Nutritive Value ◽  
Biomass Yield ◽  
Rumen Fluid ◽  
Gas Production ◽  
Average Weight ◽  
In Vitro Gas Production ◽  
Native Cattle ◽  
Maize Cob

The present study evaluated the chemical composition and in vitro ruminal digestibility of napier Pakchong 1 silage combined with maize cob and husk in different ratios. The napier Pakchong 1 grass was harvested at 45 days of maturity. The napier Pakchong 1 grass was ensiled with maize cob and husk at ratios of 1:5, 1:10 and 1:15. Three rumen fistulated Thai native cattle (White Lamphun cattle) with an average weight of 154 ± 4.7 kg were used to determine ruminal digestibility by in vitro gas-production technique. Gas production was recorded after incubating for 2, 4, 8, 12, 24, 48, 72 and 96 h. The microbial biomass yield was determined after incubating for 24 h. DM, NDF and ADF of maize cob and husk mixed with napier Pakchong 1 silage declined by increasing the proportion of napier Pakchong 1 grass, while gas production after 4–10 h of incubating maize cob and husk mixed with napier Pakchong 1 grass increased by increasing the proportion of napier Pakchong 1 grass. The metabolisable energy, organic matter digestibility and microbial biomass yield did not differ among the different ratios. The results of the study suggested a recommended ratio of maize cob and husk to napier Pakchong 1 grass of 1:10. The ensiling fermentation increased the proportion of protein in the roughage, which lead to increased in vitro gas production of roughage.

Determination of total gas production and gas profile release from a bicarbonate buffer during addition of acetic acid to a carbonated-buffered medium

1998 ◽  
Vol 1998 ◽  
pp. 58-58
Author(s):  
R. M. Mauricio ◽  
D.M.S.S Vitti ◽  
F.L. Mould ◽  
E. Owen ◽  
M.S Dhanoa ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gas Production ◽  
Gas Evolution ◽  
Gas Release ◽  
Bicarbonate Buffer ◽  
Rumen Microorganisms ◽  
Gompertz Equation ◽  
End Products

The quantity of gas accumulated in the in vitro fermentation technique (e.g. Theodorou et al., 1994) results from gaseous end-products of substrate fermentation, lysis of rumen microorganisms and CO2 released when VFA are neutralised by the carbonate-buffered medium (Beuvink and Spoelstra, 1992). However the quantity of gas produced from this acid-buffer interaction is not directly related to substrate fermentation and therefore needs to be quantified if gas evolution from substrate fermentation is to be estimated. This study examined gas release following the addition of acetic acid to a bicarbonate buffered medium and used a Gompertz equation to describe both the rate and total volume produced.

Determination of total gas production and gas profile release from a bicarbonate buffer during addition of acetic acid to a carbonated-buffered medium

1998 ◽  
Vol 1998 ◽  
pp. 58-58 ◽  
Author(s):  
R. M. Mauricio ◽  
D.M.S.S Vitti ◽  
F.L. Mould ◽  
E. Owen ◽  
M.S Dhanoa ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gas Production ◽  
Gas Evolution ◽  
Gas Release ◽  
Bicarbonate Buffer ◽  
Rumen Microorganisms ◽  
Gompertz Equation ◽  
End Products

The quantity of gas accumulated in the in vitro fermentation technique (e.g. Theodorou et al., 1994) results from gaseous end-products of substrate fermentation, lysis of rumen microorganisms and CO2 released when VFA are neutralised by the carbonate-buffered medium (Beuvink and Spoelstra, 1992). However the quantity of gas produced from this acid-buffer interaction is not directly related to substrate fermentation and therefore needs to be quantified if gas evolution from substrate fermentation is to be estimated. This study examined gas release following the addition of acetic acid to a bicarbonate buffered medium and used a Gompertz equation to describe both the rate and total volume produced.

The use of the in vitro gas production technique to investigate the effect of substrate on the partitioning between microbial biomass production and the yield of fermentation products

1999 ◽  
Vol 1999 ◽  
pp. 36-36 ◽  
Author(s):  
C. Rymer ◽  
D.I. Givens
Keyword(s):  
Microbial Biomass ◽  
Biomass Yield ◽  
Negative Relationship ◽  
Poor Quality ◽  
Gas Production ◽  
The Other ◽  
Production Technique ◽  
Fermentation Products ◽  
In Vitro Gas Production

The in vitro gas production technique has been developed as a means of predicting the degradability of feeds. However, it was pointed out by Blümmel et al. (1997) that attention should be given to the other products of the incubation as well. Degraded feeds may be incorporated directly into microbial biomass, or they may be fermented to produce VFA and gas. One of the objectives of this experiment was to determine whether the proportion of degraded substrate that was partitioned to gas production varied with different feeds. Blümmel et al. (1997) observed a negative relationship between gas yield (ml gas produced/g substrate degraded) and microbial biomass yield (mg biomass/g substrate degraded) when poor quality forages were incubated. A second objective of this work was to determine whether this relationship was also observed when higher quality feeds were used.

Biomass Yield, Chemical Composition and In Vitro Gas Production of Different Dhaincha (Sesbania spp.) Accessions from Bangladesh

2018 ◽  
Vol 35 (4) ◽  
pp. 397
Author(s):  
A.K.M. Ahsan Kabir ◽  
M. Moniruzzaman ◽  
Z. Gulshan ◽  
A.B.M. Mustanur Rahman ◽  
A.K.M Golam Sarwar
Keyword(s):  
Chemical Composition ◽  
Biomass Yield ◽  
Gas Production ◽  
In Vitro Gas Production ◽  
Sesbania Spp

scholarly journals Silage extracts used to study the mode of action of silage inoculants in ruminants

2013 ◽  
Vol 22 (1) ◽  
pp. 108-114 ◽  
Author(s):  
Richard Muck ◽  
Zwi G. Weinberg ◽  
Francisco E. Contreras-Govea
Keyword(s):  
Microbial Biomass ◽  
Lactobacillus Plantarum ◽  
Mode Of Action ◽  
Biomass Yield ◽  
Rumen Fermentation ◽  
In Vitro Fermentation ◽  
Ethanol Extracts ◽  
Silage Inoculants ◽  
In Vitro Rumen Fermentation

Lucerne and two maize crops were ensiled with and without Lactobacillus plantarum and fermented for 4 or 60 d to assess the effect of inoculant on in vitro rumen fermentation of the resulting silages. Water and 80% ethanol extracts of the silages were also analysed for effects on in vitro rumen fermentation. The inoculant affected lucerne silage characteristics but had little effect on the maize silages. In vitro fermentation of the silages showed few effects except increased microbial biomass yield (MBY) at 24 h in the inoculant-treated lucerne silages. In vitro fermentation of the lucerne silage water extracts produced no differences due to treatment except for reduced MBY in the inoculant-treated extracts. The ethanol extracts produced results inconsistent with the in vitro results of the silages. Consequently it appears that the factor in in vitro fermentation of inoculated silages causing increased MBY was in neither the water nor ethanol extracts.

scholarly journals Cultivating oyster mushrooms on red grape pomace waste enhances potential nutritional value of the spent substrate for ruminants

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246992
Author(s):  
Godfrey Mhlongo ◽  
Caven Mguvane Mnisi ◽  
Victor Mlambo
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Gas Production ◽  
Grape Pomace ◽  
Oyster Mushroom ◽  
Ruminal Fermentation ◽  
Fermentation Efficiency ◽  
Oyster Mushrooms ◽  
Red Grape

The use of red grape pomace (GP; Vitis vinifera L. var. Shiraz) as a source of beneficial bioactive compounds in ruminant diets is limited by high levels of indigestible compounds in the grape skin matrix. This problem demands innovative, inexpensive, and easy-to-use strategies that improve the digestibility of GP. The bioconversion of GP using edible oyster mushrooms (Pleurotus ostreatus) is one such strategy that has not been previously explored. Therefore, this study evaluated the effect of cultivating oyster mushrooms on GP on chemical composition and in vitro ruminal fermentation parameters of the spent mushroom substrate. The GP was inoculated with oyster mushroom spawns at 0, 200, 300, 400, or 500 g/kg, and incubated for 4 weeks. Organic matter, acid detergent lignin, sodium, manganese, cobalt, and copper linearly declined (P < 0.05) as spawn rates increased. A quadratic trend was observed for crude protein, neutral detergent fibre, acid detergent fibre, magnesium, phosphorus, and calcium content in response to increasing spawn rates. Higher spawning rates (20–50%) had a positive effect (P < 0.05) on gas production from the immediately fermentable fraction (a), rate of gas production from the slowly fermentable fraction (c) and effective gas production. However, gas production from the slowly fermentable fraction (b) and potential gas production linearly declined in response to increasing spawning rates. There was a linear increase (P < 0.05) in the immediately degradable fraction (a), while quadratic effects were observed for partition factors, effective degradability, and in vitro organic matter degradability at 48 h in response to spawning rates. It can be concluded that inoculating GP with oyster mushroom spawn reduced fibre content while increasing crude protein content and in vitro ruminal fermentation efficiency of red grape pomace. Based on the quadratic responses of partition factors at 48 hours post-inoculation, the optimum spawning rate for maximum ruminal fermentation efficiency of GP was determined to be 300 g/kg.

Sign in / Sign up

Export Citation Format

Share Document