scholarly journals Cultivating oyster mushrooms on red grape pomace waste enhances potential nutritional value of the spent substrate for ruminants

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246992
Author(s):  
Godfrey Mhlongo ◽  
Caven Mguvane Mnisi ◽  
Victor Mlambo
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Gas Production ◽  
Grape Pomace ◽  
Oyster Mushroom ◽  
Ruminal Fermentation ◽  
Fermentation Efficiency ◽  
Oyster Mushrooms ◽  
Red Grape

The use of red grape pomace (GP; Vitis vinifera L. var. Shiraz) as a source of beneficial bioactive compounds in ruminant diets is limited by high levels of indigestible compounds in the grape skin matrix. This problem demands innovative, inexpensive, and easy-to-use strategies that improve the digestibility of GP. The bioconversion of GP using edible oyster mushrooms (Pleurotus ostreatus) is one such strategy that has not been previously explored. Therefore, this study evaluated the effect of cultivating oyster mushrooms on GP on chemical composition and in vitro ruminal fermentation parameters of the spent mushroom substrate. The GP was inoculated with oyster mushroom spawns at 0, 200, 300, 400, or 500 g/kg, and incubated for 4 weeks. Organic matter, acid detergent lignin, sodium, manganese, cobalt, and copper linearly declined (P < 0.05) as spawn rates increased. A quadratic trend was observed for crude protein, neutral detergent fibre, acid detergent fibre, magnesium, phosphorus, and calcium content in response to increasing spawn rates. Higher spawning rates (20–50%) had a positive effect (P < 0.05) on gas production from the immediately fermentable fraction (a), rate of gas production from the slowly fermentable fraction (c) and effective gas production. However, gas production from the slowly fermentable fraction (b) and potential gas production linearly declined in response to increasing spawning rates. There was a linear increase (P < 0.05) in the immediately degradable fraction (a), while quadratic effects were observed for partition factors, effective degradability, and in vitro organic matter degradability at 48 h in response to spawning rates. It can be concluded that inoculating GP with oyster mushroom spawn reduced fibre content while increasing crude protein content and in vitro ruminal fermentation efficiency of red grape pomace. Based on the quadratic responses of partition factors at 48 hours post-inoculation, the optimum spawning rate for maximum ruminal fermentation efficiency of GP was determined to be 300 g/kg.

scholarly journals Nutritional Quality and In Vitro Rumen Fermentation Characteristics of Silage Prepared with Lucerne, Sweet Maize Stalk, and Their Mixtures

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1205
Author(s):  
Musen Wang ◽  
Fujin Zhang ◽  
Xinxin Zhang ◽  
Ying Yun ◽  
Lei Wang ◽  
...  
Keyword(s):  
Organic Matter ◽  
Nutritional Quality ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Fresh Matter ◽  
In Vitro Rumen Fermentation

The objective of this work was to evaluate the pH, chemical composition, minerals, vitamins, and in vitro rumen fermentation characteristics of silage prepared with lucerne, sweet maize stalk (MS), and their mixtures. Freshly chopped lucerne and MS were combined in ratios of 100:0 (M0, control), 80:20 (M20), 60:40 (M40), 40:60 (M60), 20:80 (M80), and 0:100 (M100) on a fresh matter basis. Each treatment was prepared in triplicate, and a total of eighteen silos were fermented for 65 days. After 65 days of fermentation, the pH values in M0, M20, M40, M60, M80, and M100 silages were 5.47, 4.84, 4.23, 4.13, 3.79, and 3.61, respectively. As the MS proportion in the mixtures increased, silage K, Ca, P, Na, Fe, and Cu concentrations linearly decreased (p < 0.001) and so did vitamins B5 and K1 and α-tocopherol. In vitro rumen dry matter and organic matter degradability, pH, ammonia, total volatile fatty acid, and gas production linearly decreased (p < 0.01), while neutral detergent fiber concentration linearly increased (p < 0.001), with increasing proportion of MS. The in vitro dry matter and organic matter degradability rapidly decreased when the MS percentage was ≥60%. In conclusion, the M40 silage is the most suitable for livestock utilization in local forage production considering the balance of silage pH, nutritional quality, and in vitro ruminal fermentation characteristics.

scholarly journals Sunflower cake in diets for beef cattle: digestibility, kinetics and in vitro ruminal fermentation parameters

2018 ◽  
Vol 41 (1) ◽  
pp. 39492 ◽  
Author(s):  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Luiz Henrique Xavier da Silva ◽  
Tatiana García Díaz ◽  
Antonio Ferriani Branco ◽  
Ana Lúcia Teodoro ◽  
...  
Keyword(s):  
Organic Matter ◽  
Beef Cattle ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Fermentation Products ◽  
Sunflower Cake

 The objective of this study was to evaluate the effect of the inclusion of sunflower cake replacing soybean meal in beef cattle diets on the in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM), crude protein (IVDCP) and the ruminal fermentation kinetics and parameters. The experiment was analyzed according to a completely randomized design. The treatments consisted of four levels of sunflower cake, 0, 200, 400, 600 g kg-1, replacing soybean meal in the concentrate of beef cattle diets. The coefficients of IVDDM, IVDOM and IVDCP presented a quadratic effect with the addition of sunflower cake. The soluble fraction (fraction B) degradation rate and total gas production decreased linearly with the inclusion of sunflower cake. Values of pH in ruminal fluid were higher for levels 0, 200 and 600 g kg-1 sunflower cake. Sunflower cake can replace soybean meal by up to 280 g kg-1 in the concentrate of beef cattle diets, improving the in vitro digestibility of dry matter organic, matter and crude protein. Levels above 400 g kg-1 reduce ruminal digestion rate, digestibility and release of final fermentation products. 

scholarly journals In Vitro Ruminal Fermentation and Degradability of Rice Husk on Rice Bran Substitution

2020 ◽  
Vol 24 (1) ◽  
pp. 50
Author(s):  
Rusli Fidriyanto ◽  
Roni Ridwan ◽  
Wulansih Dwi Astuti ◽  
Rohmatussolihat Rohmatussolihat ◽  
Nurul Fitri Sari ◽  
...  
Keyword(s):  
Organic Matter ◽  
Rice Husk ◽  
Rice Bran ◽  
Dry Matter ◽  
Acid Level ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Fatty Acid Production ◽  
Dry Matter Digestibility

Rice bran is a by-product of the rice milling process and has been well used as livestock feed. Rice bran is often adulterated with rice husk. The objective of this study was to evaluate the in vitro ruminal fermentation characteristics of rice bran with various compositions of rice husk and assess the relationship between rice husk addition and rice bran quality. The experiment was arranged in a completely randomized design with rice husk addition as a factor and three replications. Data of proximate value, gas production, ruminal degradability, and volatile fatty acid production were analyzed by analysis of variance. Moreover, significant effects of each treatment in the in vitro fermentation were further analyzed by Duncan's multiple range test (P<0.05). It was shown that the addition of rice husk to rice bran could increase acetic acid level, but it reduced potential gas production, gas production rate, organic matter and dry matter digestibility, and propionic acid level. Interestingly, the linear regression of dry matter digestibility, organic matter digestibility, and potential gas production showed the high adjusted R2 values. Moreover, this study also revealed that 10% of rice husk substitution on rice bran could significantly reduce the dry matter digestibility.

scholarly journals Effect of mixture of herbal plants on ruminal fermentation, degradability and gas production

2020 ◽  
Vol 43 ◽  
pp. e48549
Author(s):  
Ahmed Mahmoud Abd El Tawab ◽  
Mostafa Sayed Abdellatif Khattab ◽  
Fatma Ibrahim Hadhoud ◽  
Mahmoud Mohamed Shaaban
Keyword(s):  
Organic Matter ◽  
Ph Value ◽  
Control Diet ◽  
Short Chain Fatty Acids ◽  
Culture Technique ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Ruminant Diets ◽  
Negative Effect

Reducing livestock negative environmental impacts get great interest in last years. So, present study was carried out to determine the effect of adding different levels of mixture of thyme and celery versus salinomycin on ruminal fermentation, gas production, dry, organic matter and fiber degradation. Four experimental treatments were used by in-vitro batch culture technique, as follow: 60% CFM, 40% clover hay (control), control diet + 2.5 gm thyme + 2.5 gm celery kg-1 DM (T1), control diet + 5 gm thyme + 5 gm celery kg-1 DM (T2), control diet + 10 gm thyme + 10 gm celery kg-1 DM (T3), control diet + 0.4 gm Salinomycin kg-1 DM (T4). Ruminal pH value was significantly increased (p < 0.05) with T4 compared with other treatments. While, the T4 recorded the lowest value (p < 0.05) for microbial protein, short chain fatty acids concentrations (SCFA), total gas production, dry matter and organic matter degradability (DMd and OMd) compared with other treatments. Fiber fraction degradability (NDFd and ADFd) appeared no significant variance (p > 0.05) between control and other treatments except for T1 that recorded the lowest value (p < 0.05). It is concluded that mixture of thyme plus celery could be alternate for ionophores in the ruminant diets to enhance ruminal fermentation, reducing gas production without any negative effect on nutrients degradability.

scholarly journals Effect of substituting maize grain with cobs treated with a combination of urea and wood ash on sheep performance and rumen parameters

2021 ◽  
Vol 48 (5) ◽  
pp. 343-352
Author(s):  
A. Abdulazeez ◽  
C. M. Tsopito ◽  
O. R. Madibela
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Average Daily Gain ◽  
Wood Ash ◽  
Gas Production ◽  
Feed Conversion ◽  
Maize Grain ◽  
Maize Cobs

Effect of treating maize cobs with a combination of urea and wood ash (WA) on chemical composition, in vitro gas production, and in sacco degradability were examined in a previous study. The treatments were: (100U) =100% urea +0% wood ash, (75U25WA) = 75% urea +25% wood ash, (50U50WA) = 50% urea +50% wood ash, (25U75WA) =25 % urea + 75% wood ash and (0U0WA) = Untreated maize cobs. It was concluded in the study that, treatment 25U75WA gave better results in terms of all the parameters measured. This study aimed at determining the effect of feeding Twana sheep diets whose grain was substituted with maize cobs treated with 25%U and 75%WA on nutrient intake, digestibility, nitrogen utilization, rumen NH3, pH and performance. Tswana sheep were fed experimental diet whose maize grain was substituted with graded levels of 25U75WA in the dietary ingredients and the treatments were: 100M = 100% maize grain, 66M34C = 66% maize grain plus 34% treated maize cobs, 34M66C = 34% maize grain plus 66% treated maize cobs and 100C = 100% treated maize cobs in a 4×4 Latin Square Design (4 animals were rotated in 4 periods for all the treatments). Treatment 34M66C had crude protein intake (CPI), dry matter intake (DMI), organic matter intake (OMI) and neutral detergent fibre intake (NDFI) of 0.1334, 0.9500, 0.8921 and 0.4800 kg/d respectively (P<0.05) while the crude protein digestibility (CPD), dry matter digestibility (DMD), organic matter digestibility (OMD) and neutral detergentfibre digestibility (NDFD) were 79.57, 68.02, 66.26, and 57.8% respectively (P<0.05). All the treatments had similar (P>0.05) rumen NH3 ranging from 23.55 – 24.00 mg/ml while rumen pH were different (P<0.05) and ranged from 6.117 – 6.937 however treatment 100M had lower pH. Treatment 100M and 34M66C had average daily gain (ADG) of 0.1150 and 0.1417 kg/d, respectively (P<0.05), however, treatment 100M had lower feed conversion ratio (FCR) of 5.217 compared to 7.059 (P<0.05) for 34M66C. It was concluded that farmers should include 34M66C in the diet of Tswana sheep for optimum performance and also reduce the cost of feed.     Effet du traitement des épis de maïs avec une combinaison d'urée et de cendres de bois (CB) sur la composition chimique, la production de gaz in vitro et dans la dégradabilité de SACCO ont été examinées dans une étude précédente. Les traitements étaient: (100u) = 100% urée + 0% de cendre de bois, (75U25CB) = 75% d'urée + 25% de cendres de bois, (50u50wa) = 50% d'urée + 50% de cendre de bois, (25U75CB) = 25% d'urée + 75% de cendres bois et (0U0CB) = épis de maïs non traité. Il a été conclu dans l'étude que, le traitement 25U75CB a donné de meilleurs résultats en termes de tous les paramètres mesurés. Cette étude visait à déterminer l'effet de l'alimentation des régimes de moutons Twana dont le grain a été substitué aux épis de maïs traité avec 25% U et 75% de CB sur l'apport nutritionnel, la digestibilité, l'utilisation de l'azote, le rumen NH3, la performance de la pharmacie. Les moutons de Tswana ont été nourris à une alimentation expérimentale dont le grain de maïs était substitué par des niveaux de grade de 25U75WA dans les ingrédients alimentaires et les traitements étaient les suivants: 100m = 100% de grain de maïs, 66 m34c = 66% de grain de maïs de maïs plus 34% d'épis de maïs traité, 34m66c = 34% de maïs Grain Plus 66% COBS de maïs traité et 100c = 100% cobs de maïs traités dans un design carré latin 4 × 4 (4 animaux ont été tournés en 4 périodes pour tous les traitements). Traitement 34M66C avait une consommation de protéines brutes (CPB), une consommation de matière sèche (CMS), une consommation de matière organique (CMO) et une consommation de fibres de détergents neutres de 0,1334, 0,9500, 0,8921 et 0,4800 kg / j respectivement (p <0,05) tandis que le Digestibilité des protéines brutes (DPB), digestibilité de la matière sèche (DMS), digestibilité de la matière organique (DMO) et digestibilité de fibres de détergent neutres (DFDN) étaient de 79,57, 68,02, 66,26 et 57,8% respectivement (p <0,05). Tous les traitements avaient des analgésiques similaires (p> 0,05) Rumen NH3 allant de 23,55 à 24,00 mg / ml tandis que le pH de rumen était différent (p <0,05) et allait de 6,117 - 6,937Cependant, le traitement 100M avait un pH inférieur. Traitement 100m et 34m66C avaient un gain quotidien moyen (GQM) de 0,1150 et 0,1417 kg / j, respectivement (p <0,05), cependant, le traitement 100M avait un rapport de conversion d'alimentation inférieur (CAI) de 5,217 par rapport à 7,059 (p <0,05) pour 34 m66c. Il a été conclu que les agriculteurs comprennent 34 m66c dans le régime alimentaire des moutons de Tswana pour des performances optimales et réduisent également le coût des aliments pour animaux.

scholarly journals In Vitro Fermentation Characteristics and Methane Mitigation Responded to Flavonoid Extract Levels from Alternanthera sissoo and Dietary Ratios

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 109
Author(s):  
Sukruthai Sommai ◽  
Anusorn Cherdthong ◽  
Chanon Suntara ◽  
Sarong So ◽  
Metha Wanapat ◽  
...  
Keyword(s):  
Methane Production ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Acid Detergent Fiber ◽  
In Vitro Fermentation

Two experiments were conducted under this study: Experiment 1 was to study production yield, chemical composition, and in vitro degradability of Brazilian spinach (Alternanthera sissoo; BS) leaf and leaf + leaf-stalk at various maturity ages of 15, 30, 45, and 60 days after plantation and regrowth and Experiment 2 was to evaluate the effect of flavonoid extract from BS leaf and leaf + leaf-stalk and dietary ratios on ruminal gas production, fermentation characteristics, and in vitro degradability. Experiment 1 showed that maturity ages after planting and regrowth increased, the yield significantly increased. Increasing maturity ages significantly (p < 0.05) increased neutral detergent fiber and acid detergent fiber content and decreased crude protein content, total flavonoid (TF) content, and degradability for both leaf and leaf + leaf-stalk. Maturity ages from 15 to 30 days after plantation and regrowth resulted (p < 0.05) the highest TF content and degradability for both leaf and leaf + leaf-stalk. Thus, BS leaf and leaf + leaf-stalk samples from 15 to 30 days of age were used for flavonoid extraction and used in the Experiment 2. Experiment 2 was conducted according to a 3 × 5 factorial experiment. Three roughage to concentrate (R:C) ratios at 50:50, 40:60, and 30:70 were used, and five levels of flavonoid extract (FE) at 0, 10, 20, 30, and 40 mg of substrate dry matter (DM) were supplemented. Experiment 2 showed that R:C ratio and FE had an interaction effect only on acetate to propionate ratio. Varying R:C ratios significantly increased (p < 0.05) in vitro DM degradability, total volatile fatty acids (VFA), and propionate (C3) concentration. FE supplementation linearly (p < 0.05) increased total VFA and C3 concentration and decreased methane production and protozoal population. This study could conclude that FE from BS could effectively modulate ruminal fermentation and decrease methane production. However, in vivo study needs to elucidate in order to validate the present results.

scholarly journals Solid State Fermentation as a Tool to Stabilize and Improve Nutritive Value of Fruit and Vegetable Discards: Effect on Nutritional Composition, In Vitro Ruminal Fermentation and Organic Matter Digestibility

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1653
Author(s):  
Jone Ibarruri ◽  
Idoia Goiri ◽  
Marta Cebrián ◽  
Aser García-Rodríguez
Keyword(s):  
Organic Matter ◽  
Solid State ◽  
Solid State Fermentation ◽  
Nutritive Value ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Fruit And Vegetable ◽  
Organic Matter Digestibility

This research aimed to evaluate in vitro organic matter digestibility, fermentation characteristics and methane production of fruit and vegetable discards processed by solid state fermentation (SSF) by Rhizopus sp. Mixtures were composed of approximately 28% citric fruits, 35% other fruits and 37% vegetables. Fruit and vegetables were processed and fermented to obtain a stabilized product. Nutritional characterization and in vitro ruminal fermentation tests were performed to determine the effect of fungal bioconversion on digestibility, end products and gas production kinetics. Results indicate that SSF reduced organic matter and reducing sugars, while it increased crude protein and neutral detergent fiber, acid detergent fiber and neutral detergent insoluble protein. The in vitro gas production showed that SSF led to a reduction of the organic matter digestibility (p < 0.001), short chain fatty acids (SCFA; p = 0.003) and CH4 (p = 0.002). SSF reduced the gas production from the insoluble fraction (p = 0.001), without modifying the production rate (p = 0.676) or the lag time (p = 0.574). Regarding SCFA profile, SSF increased acetic (p = 0.020) and decreased propionic (p = 0.004) and butyric (p = 0.006) acids proportions, increasing acetic to propionic (p = 0.008) and acetic plus butyric to propionic (p = 0.011) ratios. SSF succeeded in obtaining a stabilized material enriched in protein, but at the expense of a reduction of protein availability and organic matter digestibility. These changes should be considered before including them in a ruminant’s rations.

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

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