scholarly journals Does Telomere Shortening Precede the Onset of Hypertension in Spontaneously Hypertensive Mice?

2016 ◽  
Vol 19 (5) ◽  
pp. 422-429 ◽  
Author(s):  
Christine L. Chiu ◽  
Nerissa L. Hearn ◽  
Devin Paine ◽  
Nicole Steiner ◽  
Joanne M. Lind
Keyword(s):  
Telomere Length ◽  
Genomic Dna ◽  
Heart Tissue ◽  
Biological Aging ◽  
Telomere Shortening ◽  
Spontaneously Hypertensive ◽  
Time Points ◽  
Relative Telomere Length ◽  
Before And After ◽  
Established Disease

Telomere length is widely considered as a marker of biological aging. Clinical studies have reported associations between reduced telomere length and hypertension. The aim of this study was to compare telomere length in hypertensive and normotensive mice at pre-disease and established disease time points to determine whether telomere length differs between the strains before and after the onset of disease. Genomic DNA was extracted from kidney and heart tissues of 4-, 12-, and 20-week-old male hypertensive (BPH/2J) and normotensive (BPN/3J) mice. Relative telomere length (T/S) was measured using quantitative PCR. Age was inversely correlated with telomere length in both strains. In 4-week-old pre-hypertensive animals, no difference in T/S was observed between BPH/2J and BPN/3J animals in kidney or heart tissue (kidney p = 0.14, heart p = 0.06). Once the animals had established disease, at 12 and 20 weeks, BPH/2J mice had significantly shorter telomeres when compared to their age-matched controls in both kidney (12 weeks p < 0.001 and 20 weeks p = 0.004) and heart tissues (12 weeks p < 0.001 and 20 weeks p < 0.001). This is the first study to show that differences in telomere lengths between BPH/2J and BPN/3J mice occur after the development of hypertension and do not cause hypertension in the BPH/2J mice.

Telomere Length, SIRT1, and Insulin in Male Master Athletes: The Path to Healthy Longevity?

2021 ◽  
Author(s):  
Samuel S. Aguiar ◽  
Thiago S. Rosa ◽  
Rodrigo V. P. Neves ◽  
Patrício L. A. Leite ◽  
Larissa A. Maciel ◽  
...  
Keyword(s):  
Telomere Length ◽  
Cross Sectional Study ◽  
Biological Aging ◽  
Master Athletes ◽  
Cross Sectional ◽  
Telomere Shortening ◽  
Healthy Longevity ◽  
Relative Telomere Length ◽  
Age Related ◽  
Commercial Kits

AbstractLower SIRT1 and insulin resistance are associated with accelerated telomere shortening. This study investigated whether the lifestyle of master athletes can attenuate these age-related changes and thereby slow aging. We compared insulin, SIRT1, and telomere length in highly trained male master athletes (n=52; aged 49.9±7.2 yrs) and age-matched non-athletes (n=19; aged 47.3±8.9 yrs). This is a cross-sectional study, in which all data were collected in one visit. Overnight fasted SIRT1 and insulin levels in whole blood were assessed using commercial kits. Relative telomere length was determined in leukocytes through qPCR analyses. Master athletes had higher SIRT1, lower insulin, and longer telomere length than age-matched non-athletes (p<0.05 for all). Insulin was inversely associated with SIRT1 (r=−0.38; p=0.001). Telomere length correlated positively with SIRT1 (r=0.65; p=0.001), whereas telomere length and insulin were not correlated (r=0.03; p=0.87). In conclusion, master athletes have higher SIRT1, lower insulin, and longer telomeres than age-matched non-athletes. Furthermore, SIRT1 was negatively associated with insulin and positively associated with telomere length. These findings suggest that in this sample of middle-aged participants reduced insulin, increased SIRT1 activity, and attenuation of biological aging are connected.

Abstract 278: Diet-Induced Leukocyte Telomere Shortening Correlates with Extent of Atherosclerosis

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
GENESIO KARERE ◽  
Shifra Birnbaum ◽  
Clint Christensen ◽  
Michael Mahaney ◽  
John VandeBerg ◽  
...  
Keyword(s):  
Telomere Length ◽  
Pearson Correlation ◽  
Single Copy ◽  
Developed Countries ◽  
Primate Model ◽  
Telomere Shortening ◽  
Atherosclerotic Lesions ◽  
Potential Biomarker ◽  
Significant Difference ◽  
Before And After

Introduction: Cardiovascular disease, the leading cause of death in developed countries, is commonly due to atherosclerosis. Studies have demonstrated association between leukocyte telomere shortening (LTS), extent of atherosclerotic lesions and accelerated cell senescence. Further LTS is associated with dietary intake. However, efforts to link LTS, diet and extent of lesions have been unsuccessful in humans due to difficulties controlling diet in large human population studies. To begin addressing these critical issues, we controlled dietary fat (high-fat, HF) in baboons for 2yrs - a well-developed primate model of human atherosclerosis. This is the first study in primates showing correlation of LTS with both chronic HF diet and atherosclerotic lesions. Hypothesis: We hypothesized that leukocyte telomere length decreased with chronic HF diet in baboons and is correlated with extent of atherosclerotic lesions. Methods and Results: A cohort of pedigreed baboons (n=107; females=46, males=61) was fed a HF diet for 2yrs. Absolute leukocyte telomere lengths (LTL; kb/diploid genome) were quantified by qPCR before and after diet challenge. Total telomere length was calculated by computing the ratio of telomere quantity per single copy gene quantity (baboon LIPG). Mean LTL was significantly shorter after feeding baboons a HF diet for 2 yrs (paired t test, p=0.03). Baboons (n=232) maintained on a low fat diet for 2yrs showed no significant difference in LTL (p=0.47). These findings suggest that a HF diet accelerates LTS. Further we quantified the extent of atherosclerotic lesions in baboons after 2yr HF diet and found that LTL, adjusted for age and sex, were correlated with lesions in descending aorta (Pearson correlation, r=0.19; p=0.03). Interestingly this correlation was significant in females but not in males after adjusting for age (r=0.27, p=0.03). Conclusions: LTS correlates with chronic feeding with a HF diet in baboons, is significantly correlated with arterial lesions and the correlation is sex-specific. These findings suggest that LTS may be a potential biomarker of extent of atherosclerosis.

scholarly journals Migration and stress during reproduction govern telomere dynamics in a seabird

2014 ◽  
Vol 10 (1) ◽  
pp. 20130889 ◽  
Author(s):  
Jannik Schultner ◽  
Børge Moe ◽  
Olivier Chastel ◽  
Claus Bech ◽  
Alexander S. Kitaysky
Keyword(s):  
Life History ◽  
Telomere Length ◽  
Physiological State ◽  
Telomere Shortening ◽  
Rissa Tridactyla ◽  
Life History Stages ◽  
Before And After ◽  
Migratory Patterns ◽  
Telomere Dynamics ◽  
Winter Migration

Changes in telomere length are believed to reflect changes in physiological state and life expectancy in animals. However, much remains unknown about the determinants of telomere dynamics in wild populations, and specifically the influence of conditions during highly mobile life-history stages, for example migration. We tested whether telomere dynamics were associated with migratory behaviour and/or with stress during reproduction in free-living seabirds. We induced short-term stress during reproduction in chick-rearing, black-legged kittiwakes ( Rissa tridactyla ), tracked winter migration with geolocators and measured telomere length before and after winter migration. We found that time spent at wintering grounds correlated with reduced telomere loss, while stress during reproduction accelerated telomere shortening. Our results suggest that different life-history stages interact to influence telomere length, and that migratory patterns may be important determinants of variation in an individual's telomere dynamics.

scholarly journals Comparison of the Relative Telomere Length Measured in Leukocytes and Eleven Different Human Tissues

2014 ◽  
pp. S343-S350 ◽  
Author(s):  
D. DLOUHA ◽  
J. MALUSKOVA ◽  
I. KRALOVA LESNA ◽  
V. LANSKA ◽  
J. A. HUBACEK
Keyword(s):  
Telomere Length ◽  
Peripheral Blood ◽  
Subcutaneous Fat ◽  
Relative Length ◽  
Biological Aging ◽  
Human Tissues ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Relative Telomere Length ◽  
Peripheral Leukocytes

The relative length of telomeres measured in peripheral blood leukocytes is a commonly used system marker for biological aging and can also be used as a biomarker of cardiovascular aging. However, to what extent the telomere length in peripheral leukocytes reflects telomere length in different organ tissues is still unclear. Therefore, we have measured relative telomere length (rTL) in twelve different human tissues (peripheral blood leukocytes, liver, kidney, heart, spleen, brain, skin, triceps, tongue mucosa, intercostal skeletal muscle, subcutaneous fat, and abdominal fat) from twelve cadavers (age range of 29 week of gestation to 88 years old). The highest rTL variability was observed in peripheral leukocytes, and the lowest variability was found in brain. We found a significant linear correlation between leukocyte rTL and both intercostal muscle (R=0.68, P<0.02) and liver rTL (R=0.60, P<0.05) only. High rTL variability was observed between different organs from one individual. Furthermore, we have shown that even slight DNA degradation (modeled by sonication of genomic DNA) leads to false rTL shortening. We conclude that the rTL in peripheral leukocytes is not strongly correlated with the rTL in different organs.

scholarly journals The effect of long-term ultra-endurance exercise and SOD2 genotype on telomere shortening with age

2020 ◽  
Vol 129 (4) ◽  
pp. 873-879 ◽  
Author(s):  
Barbara Hernando ◽  
Marta Gil-Barrachina ◽  
Elena Tomás-Bort ◽  
Ignacio Martinez-Navarro ◽  
Eladio Collado-Boira ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Endurance Training ◽  
Telomere Length ◽  
Endurance Exercise ◽  
Biological Aging ◽  
Acute Inflammatory Response ◽  
Telomere Shortening ◽  
Ultra Endurance ◽  
First Time

Habitual ultra-endurance exercise seems to promote telomere length maintenance, especially at older ages. In addition, the beneficial effect of ultra-endurance training on biological aging is higher in ultra-trail runners who have been engaged to ultra-endurance training during many years. Finally, and for the first time, this study shows that the SOD2 rs4880 polymorphism has a significant impact on telomere length, as well as on acute inflammatory response to a 107-km trail race.

scholarly journals Association Between Dietary Selenium Intake and Leukocyte Telomere Length in a Nationally Representative Sample of US Adults (OR11-06-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Buyun Liu ◽  
Yangbo Sun ◽  
Guifeng Xu ◽  
Shuang Rong ◽  
Wei Bao
Keyword(s):  
Telomere Length ◽  
Representative Sample ◽  
Quantitative Polymerase Chain Reaction ◽  
Antioxidant Properties ◽  
Biological Aging ◽  
Leukocyte Telomere Length ◽  
Telomere Shortening ◽  
Dietary Selenium ◽  
Selenium Intake ◽  
Nationally Representative

Abstract Objectives DNA damage induced by oxidative stress is implicated in accelerated telomere shortening, a biomarker of biological aging. Although selenium has antioxidant properties, its impact on telomere length is largely unknown. This study aimed to examine the association between dietary selenium intake and leukocyte telomere length in a nationally representative sample of US adults. Methods We included 7409 adults aged 20 years or older who participated in the National Health and Nutrition Examination Survey (NHANES) 1999–2002. Dietary selenium intake was calculated using data collected in the 24-hour dietary recall. Leukocyte telomere length was assayed using the quantitative polymerase chain reaction method. The association between selenium intake and telomere length was estimated by weighted linear regression models adjusting for demographic, socioeconomic and lifestyle factors, body mass index, supplements intake, and leukocyte cell type composition. Results The average dietary selenium intake was 109.1 mg/d (standard error [SE] 1.15). We didn't find a significant association between dietary selenium intake and telomere length in US adults. The average telomere length (SE) was 1.01 (0.02), 1.01 (0.01), and 1.04 (0.01) across increasing tertiles of dietary selenium intake. However, a significant interaction was observed for age (P = 0.02). Among individuals aged 20–44 years, the β coefficient of log-transformed telomere length, compared to lowest tertile of dietary selenium intake, was −0.041 (SE 0.012, P = 0.002) and −0.033 (SE 0.018, P = 0.07) for middle tertile and the highest tertile of selenium intake, respectively. The corresponding β coefficient was 0.009 (SE 0.016, P = 0.59) and −0.001 (SE 0.012, P = 0.95), respectively, for adults 45–64 years old, and 0.017 (SE 0.015, P = 0.28) and 0.059 (SE 0.021, P = 0.01), respectively, for those aged 65 years or older. The results were not appreciably changed even after additionally adjustment for dietary intake of vitamin A, vitamin E, and zinc. Conclusions The association between dietary selenium intake and telomere length differed significantly by age groups, indicating that higher selenium intake may prevent telomere shortening in older adults but not in younger or middle-aged adults. Further studies about the underlying mechanisms are warranted. Funding Sources NA.

scholarly journals Stress reactivity elicits a tissue-specific reduction in telomere length in ageing zebrafish (Danio rerio)

2020 ◽  
Author(s):  
James R. Evans ◽  
Jose V. Torres-Pérez ◽  
Maria Elena Miletto Petrazzini ◽  
Riva Riley ◽  
Caroline H. Brennan
Keyword(s):  
Telomere Length ◽  
Stress Reactivity ◽  
Quantitative Polymerase Chain Reaction ◽  
Heart Tissue ◽  
Telomere Shortening ◽  
Tissue Specific ◽  
Age Related ◽  
Specific Manner ◽  
Polymerase Chain ◽  
The Relationship

ABSTRACTTelomere length reflects cellular ageing. Increased telomere shortening in leukocytes is associated with a range of neurodegenerative and cardiovascular diseases, the onset and progression of which may be mediated by behavioural traits such as anxiety and stress reactivity. However, the effects of the hypothalamus-pituitary-adrenal axis stress response are shown to be tissue specific. As such, leukocyte telomere length may not give an accurate measure of the relationship between stress-reactivity and telomere length in disease relevant tissues. To test the hypothesis that stress-reactivity contributes to age-related telomere shortening in a tissue specific manner, we examined the correlation between telomere length in heart and brain tissue and stress-reactivity in a population of young (6-9 month) and ageing (18 month) zebrafish. Stress-reactivity was assessed by tank diving, a zebrafish version of the rodent open-field test, and through gene expression. Telomere length was assessed using quantitative polymerase chain reaction. We show that ageing zebrafish have shorter telomeres in both heart and brain. Telomere length is inversely related to stress-reactivity in heart but not brain of ageing individuals. These data support the hypotheses that an anxious predisposition contributes to telomere shortening in heart tissue, and by extension age-related heart disease, and that stress-reactivity contributes to age-related telomere shortening in a tissue-specific manner.

scholarly journals Shorter telomere length is associated with a more recent diagnosis of coeliac disease

2018 ◽  
Author(s):  
Pierre Goorkiz ◽  
Nerissa L Hearn ◽  
Saskia van der Kooi ◽  
Christine L Chiu ◽  
Joanne M Lind
Keyword(s):  
Coeliac Disease ◽  
Telomere Length ◽  
Mucosal Damage ◽  
Gluten Free ◽  
Telomere Shortening ◽  
Relative Telomere Length ◽  
Significant Difference ◽  
Autoimmune Conditions ◽  
Recent Diagnosis ◽  
Inflammatory Immune Response

AbstractBackgroundCoeliac disease (CD) is an autoimmune disease that causes an inappropriate inflammatory immune response to dietary gluten. Telomere length is a marker of biological ageing and is reduced in several autoimmune conditions. This observational study measured salivary telomere length (TL) in gluten-free diet (GFD) treated CD individuals to determine if CD, and length of time on a GFD, is associated with salivary TL.MethodsClinical and demographic information was collected from CD individuals currently treated with a GFD and healthy non-affected controls. Only participants aged under 35 years at recruitment were included. Relative telomere length was measured using quantitative PCR in oral mucosa collected from saliva. Linear regression was used to determine whether salivary TL was associated with CD, or length of time on a GFD, adjusting for age and sex.ResultsThis study included 79 participants, 52 GFD-treated CD and 27 non-affected controls. No significant difference in salivary TL between individuals with treated CD and controls was found. Within CD individuals, salivary TL was associated with length of time on a GFD, with individuals who started a GFD ≤3 years ago having shorter salivary TL compared to those who started a GFD > 3 years ago (0.37±0.05 vs 0.50±0.04; p=0.002).ConclusionOur findings indicate that salivary TL shorten while CD is untreated, however following treatment on a GFD, they appear to recover to those seen in unaffected controls. This highlights the importance of early diagnosis and initiation of GFD to minimise mucosal damage and telomere shortening, to enable TL to recover.

scholarly journals Influence of music therapy on the telomere length: a brief review and a pilot study

Biologija ◽  
2019 ◽  
Vol 64 (4) ◽  
Author(s):  
Irina M. Spivak ◽  
Tatiana Yu. Smirnova ◽  
Arina S. Urazova ◽  
Andrey L. Runov ◽  
Anastasia A. Vasilishina ◽  
...  
Keyword(s):  
Music Therapy ◽  
Telomere Length ◽  
Music Perception ◽  
Classical Music ◽  
Telomerase Activity ◽  
Adaptive Performance ◽  
Telomere Shortening ◽  
Age Related ◽  
Psychological Training ◽  
Before And After

Relation between music perception and telomere length is discussed. Telomeres are now considered as markers of general health and as possible predictor of life expectancy, while their length correlates with either the risk of age-related pathologies or with higher adaptive performance. The ability to withstand agerelated telomere shortening by means of practicing psychological training is discussed. Problems and prospects of present-day music therapy are reviewed, as well as absence of studies of alteration of telomere length, related to music perception. Sixty-three practically healthy young (aged 24 ± 5 years) randomly selected Russian-speaking students were divided into three subgroups, each of which listened to music of a specific type for 14 days, 90 minutes each day. Before and after the two-week course, the telomere length and telomerase activity in blood samples were measured. A limited but statistically reliable increase in the telomere length and in telomerase activity is demonstrated to have occurred as a result of completing the music course. Classical music tends to bring about less stress, positively affect one’s mood, and physiology, and, finally, such essential aging markers as telomerase activity and the telomere length. Non-classical music conditioned manifestation of some stress, which tended to affect the telomere length in a negative way. In order to cope with this difficulty, telomerase was activated.

Telomere shortening velocity of patients administered with hypnotics is accelerated in a gender-differential manner

2020 ◽  
pp. 1-6
Author(s):  
Toyoki Maeda ◽  
Takahiko Horiuchi ◽  
Naoki Makino
Keyword(s):  
Telomere Length ◽  
The Other ◽  
Biological Aging ◽  
Shortening Velocity ◽  
Telomere Shortening ◽  
Female Patients ◽  
Associated Diseases ◽  
Other Hand ◽  
Male Patients ◽  
Gender Differential

The telomere length and its distribution were compared between patients administered with and without hypnotics to see if regular administration of hypnotics is associated with their aging-related somatic telomere shortening. Male patients presented significant shortening of telomere length of circulating leukocytes in association with age (–41.9 bp/year, p = 0.045) in contrast with controls (–18.3 kb/year, p = 0.155). On the other hand, female patients presented no significant shortening of telomere length with aging (–16.4 bp/year, p = 0.372) in contrast with controls (–55.9 bp/year, p = 0.00005). These results suggested that regular administration of hypnotics is associated with aging progression in a gender-related manner. The administration of hypnotics could be an indicator as the somatic aging status and for the screening of background lifestyle-associated diseases promoting biological aging.

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