High-performance packed glass-lined stainless steel capillary column for microcolumn liquid chromatography

1985 ◽  
Vol 57 (12) ◽  
pp. 2235-2239 ◽  
Author(s):  
Masaharu. Konishi ◽  
Yoshio. Mori ◽  
Tameyuki. Amano
Keyword(s):  
Stainless Steel ◽  
Liquid Chromatography ◽  
High Performance ◽  
Capillary Column ◽  
Microcolumn Liquid Chromatography ◽  
Stainless Steel Capillary

Overcoming Gas Sampling Problems: Analysis of Volatiles Using Capillary Column Needles

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 643f-643
Author(s):  
Weimin Deng ◽  
Randolph M. Beaudry
Keyword(s):  
Stainless Steel ◽  
Capillary Column ◽  
Dependent Manner ◽  
Stainless Steel Capillary ◽  
Stainless Steel Needle ◽  
Injection Volume ◽  
Volatile Analysis ◽  
Gas Tight ◽  
Sampling Problems ◽  
Time Injection

Sampling factors that could affect gas chromatograph (GC) response for volatile analysis such as syringe pumping time, injection volume, needle length, temperature, and the type of volatile were investigated. Capillary GC column segments (steel and glass) were installed in gas-tight syringes and used as needles for volatile analysis. Standard stainless-steel needles were also used. Hexylacetate, ethyl-2-methylbutyrate, 6-methyl-5-hepten-2-one, and butanol standard were measured. The number of pumps required to maximize GC response for each needle–volatile combination was determined. Maximal GC response for hexylacetate using standard stainless steel, capillary glass, and capillary steel needles required 10, 20 and 30 pumps, respectively. However, for butanol measurement, the optimal syringe pump number was 5 to 10 for all needle types. The use of a capillary needle resulted in an increase in GC response in the range of 3- to 15-fold relative to a standard stainless steel needle. Injection volume affected GC response in a needle-and volatile-dependent manner. In no case did injection volume vs. GC response extrapolate through origin. The GC response for capillary column needles increased as temperature decreased. Capillary column needles may be useful tools for analysis of volatiles that readily partition into the column coating.

Microcolumn liquid chromatography: a tool of potential significance in biomedical research.

1980 ◽  
Vol 26 (10) ◽  
pp. 1474-1479 ◽  
Author(s):  
M Novotny
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biomedical Research ◽  
High Performance ◽  
Recent Progress ◽  
High Efficiency ◽  
Potential Significance ◽  
Microcolumn Liquid Chromatography ◽  
High Efficiency Separations

Abstract Recent progress in the area of microcolumn HPLC is reviewed with an emphasis on biomedically important directions. The potential of high efficiency separations, additional advantages of solvent economy, and the development of new detection and ancillary techniques of high-performance liquid chromatography are also discussed.

scholarly journals Analysis of Quinapril by Two Solvent-Saving Methods: Application of Capillary Column High-Performance Liquid Chromatography with Ultraviolet Absorbance Detection and LDI-TOF-MS

2010 ◽  
Vol 93 (4) ◽  
pp. 1201-1206
Author(s):  
Chi-Yu Lu ◽  
Yi-Rou Wang ◽  
Su-Hwei Chen ◽  
Chia-Hsien Feng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Capillary Column ◽  
Enzyme Inhibitor ◽  
Internal Standard ◽  
Ionization Time ◽  
Absorbance Detection ◽  
Tof Ms

Abstract A capillary column high-performance liquid chromatography (CapLC) method and a laser desorption ionization-time of flight (LDI-TOF)-MS method are described for the determination of quinapril, an angiotensin-converting enzyme inhibitor. Effective separation was achieved by using a C18 capillary column at a flow rate of 10 L/min. For CapLC, quinapril and 7-hydroxycoumarin (internal standard) were detected at 210 and 320 nm, respectively. Phenformin replaced 7-hydroxycoumarin as the internal standard for the LDI-TOF-MS method successfully developed to detect quinapril. The calibration curves showed good linearity in the range of 1100 g/mL in these two methods. For high throughput purposes, the LDI-TOF-MS method was simpler and faster than the CapLC method. Both green methods were suitably validated and successfully applied to determine quinapril in commercial tablets.

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