scholarly journals Shift Reagents for Multidimensional Ion Mobility Spectrometry-Mass Spectrometry Analysis of Complex Peptide Mixtures: Evaluation of 18-Crown-6 Ether Complexes

2011 ◽  
Vol 83 (13) ◽  
pp. 5377-5385 ◽  
Author(s):  
Brian C. Bohrer ◽  
David E. Clemmer
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Peptide Mixtures ◽  
Shift Reagents

scholarly journals Ion mobility-mass spectrometry analysis of isomeric carbohydrate precursor ions

2009 ◽  
Vol 394 (7) ◽  
pp. 1853-1867 ◽  
Author(s):  
Maolei Zhu ◽  
Brad Bendiak ◽  
Brian Clowers ◽  
Herbert H. Hill
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Mass Spectrometry Analysis ◽  
Ion Mobility Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Precursor Ions

Effectiveness and Limitations of Computational Chemistry and Mass Spectrometry in the Rational Design of Target-specific Shift Reagents for Ion Mobility Spectrometry

ChemPhysChem ◽  
2018 ◽  
Vol 19 (21) ◽  
pp. 2921-2930 ◽  
Author(s):  
Christopher Kune ◽  
Jean R. N. Haler ◽  
Johann Far ◽  
Edwin De Pauw
Keyword(s):  
Mass Spectrometry ◽  
Computational Chemistry ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Rational Design ◽  
Shift Reagents

scholarly journals Traveling-Wave Ion Mobility Mass Spectrometry Analysis of Isomeric Modified Peptides Arising from Chemical Cross-Linking

2010 ◽  
Vol 21 (12) ◽  
pp. 2062-2069 ◽  
Author(s):  
Luiz F.A. Santos ◽  
Amadeu H. Iglesias ◽  
Eduardo J. Pilau ◽  
Alexandre F. Gomes ◽  
Fabio C. Gozzo
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Traveling Wave ◽  
Mass Spectrometry Analysis ◽  
Cross Linking ◽  
Ion Mobility Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Modified Peptides ◽  
Traveling Wave Ion Mobility ◽  
Chemical Cross Linking

scholarly journals Getting more out of co-immunoprecipitation mass spectrometry experiments by reducing interference using FAIMS.

2021 ◽  
Author(s):  
Ching-Seng Ang ◽  
Joanna Sacharz ◽  
Michael G Leeming ◽  
Shuai Nie ◽  
Swati Varshney ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Protein Complexes ◽  
Affinity Purification ◽  
Mass Spectrometry Analysis ◽  
Modern Biology ◽  
Spectrometry Analysis ◽  
Affinity Enrichment ◽  
Unbiased Manner ◽  
Gas Phase Ion

Co-immunoprecipitation of proteins coupled to mass spectrometry has transformed modern biology understanding of protein interaction networks. These approaches exploit the selective isolation of tagged proteins by affinity enrichment / purification to identify protein binding partners at scale and in an unbiased manner. In instances where a suitable antibody is not be available it is common to graft synthetic tags such as FLAG or His Tags onto target protein sequences allowing the use of commercially available and validated antibodies for affinity purification. To allow the selective elution of protein complexes competitive displacement using a large molar excess of the tag peptide is widely used. Yet, this creates downstream challenges for the mass spectrometry analysis due to the presence of large quantities of a contaminating peptide. Here, we demonstrate that Field Asymmetric Ion Mobility Spectrometry (FAIMS), a gas phase ion separation device can be applied to FLAG-Tag and His-Tag pull down assay to increase the depth of protein coverage in these experiments. By excluding tag peptides based on their ion mobility profiles we demonstrate that single compensation voltage, or stepped compensation voltages strategies can significantly increase the coverage of total proteins by up to 2.5-fold and unique proteins by up to 15-fold versus experiments that do not use FAIMS. Combined these results highlight FAIMS is able to improve proteome depth by excluding interfering peptides without the need for additional sample handling or altering sample preparation protocols.

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