Development of an Optical Biosensor Based Immunoassay to Screen Infant Formula Milk Samples for Adulteration with Melamine

The aim of this study was to investigate the potential of using Al-Holy–Rasco (AR) medium, a novel broth for detection and isolation of Cronobacter spp. in infant formula milk (IFM). The new medium's composition is generic brain heart infusion broth with the addition of 1% NaCl, 15% sucrose, and 0.80 g/liter sodium deoxycholate as selective ingredients. AR broth outperformed Enterobacteriaceae enrichment broth (EE), Enterobacter sakazakii enrichment broth (ESE), modified lauryl sulfate broth, and milk as enrichment media to stimulate the growth of a cocktail of 10 strains of Cronobacter. Additionally, AR broth significantly suppressed the growth of competing non-Cronobacter Enterobacteriaceae as compared with EE, ESE, modified lauryl sulfate broth, and milk. The recovery of desiccated Cronobacter (1 to 5,000 CFU/100 g) from powdered IFM in the presence of competing non-Cronobacter Enterobacteriaceae was determined by EE, ESE, and AR broth with 10 and 15% sucrose. AR broth with 15% sucrose outperformed all other examined broths and recovered Cronobacter from all samples tested at all Cronobacter concentrations. AR broth must be validated before it can be used for rapid detection and isolation of Cronobacter from powdered IFM.

Download Full-text

Implementation of Government Regulation Number 33 of 2012 concerning Exclusive Breastfeeding as a Form of Legal Protection for Babies Against the Promotion of Infant Formula Milk in Palembang City

SOEPRA ◽

10.24167/shk.v4i2.1491 ◽

2019 ◽

Vol 4 (2) ◽

pp. 263

Author(s):

Anastasya Megasstuti Gunadi ◽

Endang Widyorini ◽

Yovita Indrayati

Keyword(s):

Infant Formula ◽

Exclusive Breastfeeding ◽

Government Regulation ◽

Legal Protection ◽

Formula Milk ◽

Infant Formula Milk

Download Full-text

Evaluation of Heavy Metals Profile in Different Brands of Infant Food Nutrition

Chemical Science International Journal ◽

10.9734/csji/2019/v27i330116 ◽

2019 ◽

pp. 1-9

Author(s):

Ogochukwu Augustina Onuorah ◽

Rufus Sha'ato ◽

Fina Okeke ◽

Adekola Omololu Atanda ◽

Agbele Idowu Elijah ◽

...

Keyword(s):

Trace Metals ◽

Infant Formula ◽

Daily Intake ◽

World Health ◽

Infant Food ◽

Formula Milk ◽

Infant Formulae ◽

Infant Formula Milk ◽

Significant Difference ◽

Toxic Trace Metals

The concentrations of five essential metals in six selected (NAN, Cowbell, MY BOY, CERELAC, FRISO GOLD and NUTREND) infant formulae sold in Makurdi metropolis, were determined using Atomic Absorption Spectrophotometer. Data collected were subjected to Analysis of Variance using SPSS 24 statistical software package. Significant differences in means were separated using Duncan Multiple Range Test. There was significant difference in the levels of the trace metals in the different brands of infant formula milk. The results of the toxic trace metals show that Cr has the highest concentration of 6.175 ±0.001 mg/kg (Cowbell), when compared to other toxic trace metals analyzed. Milk based infant formula showed copper concentration ranged from 2.55±0.001– 6.357±0.001mg/kg, which was higher than the provisional tolerable daily intake of copper that is 3 mg/kg. Chromium, Copper, Manganese and lead were detected in the six brands of infant formula milk but they were below the limits set by World Health Organization (WHO). However, the intake levels of Cd in this study from these concentrations were below the WHO limits. These results validate and in some cases counter earlier reports on the composition of infant formula found in our local markets. Therefore, the concentrations of the studied elements were within the safe limits recommended by National Industrial Standard and the Institute of Medicine for foods but higher concentrations of trace metals in some brands of infant formulae above the recommended limit pose a risk of bioaccumulation.

Download Full-text