Levels of Trace Elements in Human Breast Milk in Jordan: a Comparison with Infant Formula Milk Powder

Author(s):  
Yahya R. Tahboub ◽  
Adnan M. Massadeh ◽  
Nihaya A. Al-sheyab ◽  
Diab El shrafat ◽  
Israa A. Nsserat
2020 ◽  
Vol 10 (17) ◽  
pp. 6135
Author(s):  
Federica Dal Bello ◽  
Enrica Mecarelli ◽  
Daniela Gastaldi ◽  
Francesco Savino ◽  
Claudio Medana

Leptin is a 16 kDa lipophilic protein hormone secreted by adipocytes and its most significant function is to inform the brain with negative feedback that regulates food intake. Recently the protein found in human breast milk was related to breast feeding and onset of obesity, and the evidence of a low probability to develop pediatric obesity in children fed with breast milk was also confirmed. Since leptin could have a critical role, its quantitation both in human breast, bovine milk and in infant formula products is interesting. For this reason, we developed an analytical method based on immunoaffinity purification followed by an analysis with nano-High Pressure Liquid Chromatography coupled with High Resolution Mass Spectrometry analyzer (nano-HPLC-HRMS) to identify and quantify leptin in milk samples and performed a pilot study using samples of human breast milk, bovine milk and infant formulas. With an obtained lower limit of quantitation (LLOQ) of 100 ng mL−1 we quantified leptin in human breast milk finding an average of 6.70 ng mL−1. Our results show that leptin was under LLOQ both in bovine milk and in infant formula products. In conclusion, the developed analytical method here described was suitable to quantify leptin in milk samples with a good sensitivity and selectivity, and without the use of radioactive reagents.


2008 ◽  
Vol 42 (22) ◽  
pp. 8597-8602 ◽  
Author(s):  
Lin Tao ◽  
Jing Ma ◽  
Tatsuya Kunisue ◽  
E. Laurence Libelo ◽  
Shinsuke Tanabe ◽  
...  

1997 ◽  
Vol XLI (3) ◽  
pp. 163
Author(s):  
BRENDAN O??HARE ◽  
JERROLD LERMAN ◽  
JUNKO ENDO ◽  
ERNEST CUTZ

2019 ◽  
Vol 5 (1) ◽  
pp. 8-10
Author(s):  
Moudah Alhindi ◽  
Hadohum Musallam ◽  
Esam Elhefian

Human milk is a complex fluid, which contains a number of constituents such as fats, proteins and vitamins, in addition to other compounds. These nutrients are needed for infant protection against infections and diseases. This study was undertaken to evaluate some physicochemical properties and biochemical constituents content of human milk samples (based on the gender of the breast feeding infant). This includes acidity, density and viscosity, as well as lactose, protein, calcium and magnesium contents. Results showed that the highest value of viscosity as well as the highest percentages of protein and acidity were recorded for human breast milk for a boy (2.212 cP, 1.237% and 0.02% respectively) while close values for density and other biochemical contents were recorded for both types of samples. However, only the change in viscosity was found to be significant (p?0.05). Variations in some properties of human milk depending on the infant' sex could be of benefit for developing infant formula taking infant’s gender into consideration.


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