Rapid Quantitative Profiling of N-Glycan by the Glycan-Labeling Method Using 3-Aminoquinoline/α-Cyano-4-hydroxycinnamic Acid

The involvement of nucleus in the maturation processes of Dengue-2 virus in a mosquito cell line, C6/36 cells, has been identified by the electron microscopy and immunocytochemistry. The C6/36 cells were obtained from ATCC and maintained in MEM culture medium containing 10% fetal bovine serum at 28°C. The cell suspensions or cells grown on teflon-coated coverslips were infected with Dengue-2 virus (107/ml) for various time periods of 2 hours, 3, 6, 8, and 10 days. The cells were then fixed in buffered 1.5% glutaraldehyde, and washed in acetone before immunolabeled with monoclonal antibody. An indirect immunocytochemical labeling method of avidin-biotin complex (ABC) conjugated with peroxidase or gold particles (20 nm in diameter) and a flat embedding technique were used to localize the virus particles.At early stages of infections (before 3 days), there were no virion particles detected. After 6 days and on of infections, cytopathic effect (CPE) was observed and showed positive immuno-peroxidase reactions under the light and electron microscopies.

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In Vitro and in Vivo Comparison of Binding of 99m-Tc-Iabeled Anti-CEA MAb F33-104 with 99m-Tc-labeled Anti-CEA MAb BW431/26

Nuklearmedizin ◽

10.1055/s-0038-1632202 ◽

1999 ◽

Vol 38 (04) ◽

pp. 115-119

Author(s):

N. Oriuchi ◽

S. Sugiyama ◽

M. Kuroki ◽

Y. Matsuoka ◽

S. Tanada ◽

...

Keyword(s):

Nude Mice ◽

Binding Capacity ◽

Colon Adenocarcinoma ◽

Human Colon ◽

Cell Binding ◽

Vitro Binding ◽

Labeling Method ◽

Human Colon Adenocarcinoma

Summary Aim: The purpose of this study was to assess the potential for radioimmunodetection (RAID) of murine anti-carcinoembryonic antigen (CEA) monoclonal antibody (MAb) F33-104 labeled with technetium-99m (99m-Tc) by a reduction-mediated labeling method. Methods: The binding capacity of 99m-Tc-labeled anti-CEA MAb F33-104 with CEA by means of in vitro procedures such as immunoradiometric assay and cell binding assay and the biodistribution of 99m-Tc-labeled anti-CEA MAb F33-104 in normal nude mice and nude mice bearing human colon adenocarcinoma LS180 tumor were investigated and compared with 99m-Tc-labeled anti-CEA MAb BW431/26. Results: The in vitro binding rate of 99m-Tc-labeled anti-CEA MAb F33-104 with CEA in solution and attached to the cell membrane was significantly higher than 99m-Tclabeled anti-CEA MAb BW431/261 (31.4 ± 0.95% vs. 11.9 ± 0.55% at 100 ng/mL of soluble CEA, 83.5 ± 2.84% vs. 54.0 ± 2.54% at 107 of LS 180 cells). In vivo, accumulation of 99m-Tc-labeled anti-CEA MAb F33-104 was higher at 18 h postinjection than 99m-Tc-labeled anti-CEA MAb BW431/26 (20.1 ± 3.50% ID/g vs. 14.4 ± 3.30% ID/g). 99m-Tcactivity in the kidneys of nude mice bearing tumor was higher at 18 h postinjection than at 3 h (12.8 ± 2.10% ID/g vs. 8.01 ± 2.40% ID/g of 99m-Tc-labeled anti-CEA MAb F33-104, 10.7 ± 1.70% ID/g vs. 8.10 ± 1.75% ID/g of 99m-Tc-labeled anti-CEA MAb BW431/26). Conclusion: 99m-Tc-labeled anti-CEA MAb F33-104 is a potential novel agent for RAID of recurrent colorectal cancer.

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Faculty Opinions recommendation of Single bead labeling method for combining confocal fluorescence on-bead screening and solution validation of tagged one-bead one-compound libraries.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.2036976.1615077 ◽

2010 ◽

Author(s):

Dehua Pei ◽

Tao Liu

Keyword(s):

Compound Libraries ◽

Confocal Fluorescence ◽

Single Bead ◽

Labeling Method

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Rapid Detection of σ‐Hydroxycinnamic Acid and Beta‐Glucosidase in Melilotus alba 1

Crop Science ◽

10.2135/cropsci1970.0011183x001000050006x ◽

1970 ◽

Vol 10 (5) ◽

pp. 479-481 ◽

Cited By ~ 4

Author(s):

F. A. Haskins ◽

H. J. Gorz

Keyword(s):

Rapid Detection ◽

Hydroxycinnamic Acid ◽

Melilotus Alba ◽

Beta Glucosidase

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A dual-isotope-labeling method of studying the bioavailability of hexaglutamyl folic acid relative to that of monoglutamyl folic acid in humans by using multiple orally administered low doses

American Journal of Clinical Nutrition ◽

10.1093/ajcn/84.5.1128 ◽

2006 ◽

Vol 84 (5) ◽

pp. 1128-1133 ◽

Cited By ~ 14

Author(s):

Alida Melse-Boonstra ◽

Petra Verhoef ◽

Clive E West ◽

Johannes A van Rhijn ◽

Richard B van Breemen ◽

...

Keyword(s):

Folic Acid ◽

Isotope Labeling ◽

Low Doses ◽

Dual Isotope ◽

Labeling Method

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Moment-to-Moment Continuous Attention Fluctuation Monitoring through Consumer-Grade EEG Device

Sensors ◽

10.3390/s21103419 ◽

2021 ◽

Vol 21 (10) ◽

pp. 3419

Author(s):

Shan Zhang ◽

Zihan Yan ◽

Shardul Sapkota ◽

Shengdong Zhao ◽

Wei Tsang Ooi

Keyword(s):

Ground Truth ◽

Detection Accuracy ◽

Continuous Performance Task ◽

Eeg Signals ◽

Gradual Onset ◽

Machine Learning Approach ◽

A New Technique ◽

Thought Probes ◽

Labeling Method ◽

Fluctuation Measurement

While numerous studies have explored using various sensing techniques to measure attention states, moment-to-moment attention fluctuation measurement is unavailable. To bridge this gap, we applied a novel paradigm in psychology, the gradual-onset continuous performance task (gradCPT), to collect the ground truth of attention states. GradCPT allows for the precise labeling of attention fluctuation on an 800 ms time scale. We then developed a new technique for measuring continuous attention fluctuation, based on a machine learning approach that uses the spectral properties of EEG signals as the main features. We demonstrated that, even using a consumer grade EEG device, the detection accuracy of moment-to-moment attention fluctuations was 73.49%. Next, we empirically validated our technique in a video learning scenario and found that our technique match with the classification obtained through thought probes, with an average F1 score of 0.77. Our results suggest the effectiveness of using gradCPT as a ground truth labeling method and the feasibility of using consumer-grade EEG devices for continuous attention fluctuation detection.

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