Highly Sensitive MALDI Analyses of Glycans by a New Aminoquinoline-Labeling Method Using 3-Aminoquinoline/α-Cyano-4-hydroxycinnamic Acid Liquid Matrix

2011 ◽  
Vol 83 (10) ◽  
pp. 3663-3667 ◽  
Author(s):  
Kaoru Kaneshiro ◽  
Yuko Fukuyama ◽  
Shinichi Iwamoto ◽  
Sadanori Sekiya ◽  
Koichi Tanaka
Keyword(s):  
Hydroxycinnamic Acid ◽  
Liquid Matrix ◽  
Highly Sensitive ◽  
Labeling Method

Rapid Quantitative Profiling of N-Glycan by the Glycan-Labeling Method Using 3-Aminoquinoline/α-Cyano-4-hydroxycinnamic Acid

2012 ◽  
Vol 84 (16) ◽  
pp. 7146-7151 ◽  
Author(s):  
Kaoru Kaneshiro ◽  
Makoto Watanabe ◽  
Kazuya Terasawa ◽  
Hiromasa Uchimura ◽  
Yuko Fukuyama ◽  
...  
Keyword(s):  
Hydroxycinnamic Acid ◽  
Labeling Method ◽  
Quantitative Profiling

scholarly journals Estrogen Receptor β-Mediated Inhibition of Male Germ Cell Line Development in Mice by Endogenous Estrogens during Perinatal Life

Endocrinology ◽  
2004 ◽  
Vol 145 (7) ◽  
pp. 3395-3403 ◽  
Author(s):  
Géraldine Delbès ◽  
Christine Levacher ◽  
Catherine Pairault ◽  
Chrystèle Racine ◽  
Clotilde Duquenne ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Germ Cell ◽  
Experimental Studies ◽  
Cell Lineage ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Testicular Development ◽  
Fetal Life ◽  
Germ Cell Line ◽  
Highly Sensitive ◽  
Labeling Method

Abstract Epidemiological, clinical, and experimental studies have suggested that excessive exposure to estrogens during fetal/neonatal life can lead to reproductive disorders and sperm abnormalities in adulthood. However, it is unknown whether endogenous concentrations of estrogens affect the establishment of the male fetal germ cell lineage. We addressed this question by studying the testicular development of mice in which the estrogen receptor (ER) β or the ERα gene was inactivated. The homozygous inactivation of ERβ (ERβ−/−) increased the number of gonocytes by 50% in 2- and 6-d-old neonates. The numbers of Sertoli and Leydig cells and the level of testicular testosterone production were unaffected, suggesting that estrogens act directly on the gonocytes. The increase in the number of gonocytes did not occur during fetal life but instead occurred just after birth, when gonocytes resumed mitosis and apoptosis. It seems to result from a decrease in the apoptosis rate evaluated by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method and cleaved caspase-3 immunohistochemical detection. Last, mice heterozygous for the ERβ gene inactivation behaved similarly to their ERβ−/− littermates in terms of the number of gonocytes, apoptosis, and mitosis, suggesting that these cells are highly sensitive to the binding of estrogens to ERβ. ERα inactivation had no effect on the number of neonatal gonocytes and Sertoli cells. In conclusion, this study provides the first demonstration that endogenous estrogens can physiologically inhibit germ cell growth in the male. This finding may have important implications concerning the potential action of environmental estrogens.

Structural Analysis of N-Glycans by the Glycan-Labeling Method Using 3-Aminoquinoline-Based Liquid Matrix in Negative-Ion MALDI-MS

2012 ◽  
Vol 84 (21) ◽  
pp. 9453-9461 ◽  
Author(s):  
Takashi Nishikaze ◽  
Kaoru Kaneshiro ◽  
Shin-ichirou Kawabata ◽  
Koichi Tanaka
Keyword(s):  
Structural Analysis ◽  
Maldi Ms ◽  
Negative Ion ◽  
Liquid Matrix ◽  
Labeling Method

scholarly journals Food Analysis: Task specific ionic liquids for separation of nickel and cadmium from olive oil samples by thermal ultrasound-assisted dispersive multiphasic microextraction

2019 ◽  
Vol 2 (2) ◽  
pp. 55-64 ◽  
Author(s):  
Asian Khaligh ◽  
Hamid Shirkhanloo
Keyword(s):  
Olive Oil ◽  
Limit Of Detection ◽  
Hydroxycinnamic Acid ◽  
Extraction And Separation ◽  
Analysis Task ◽  
Highly Sensitive ◽  
Ultrasound Assisted ◽  
Optimized Conditions ◽  
Extracting Solvent ◽  
Task Specific Ionic Liquid

A novel task-specific ionic liquid (TSILs) was used for highly sensitive extraction and separation of nickel and cadmium in olive oil by thermal ultrasound-assisted dispersive multiphasic microextraction (TUSA-DMPμE). By proposed method, a mixture containing of hydrophilic TSILs (α- Cyano-4-hydroxycinnamic acid diethylamine; [CHCA] [DEA] and 1-(2-Hydroxyethyl)-3-methylimidazolium tetrafluoroborate; [HEMIM][BF4]) as a complexing and extracting solvent, acetone as a dispersant of TSILs was added to diluted olive oil with n-hexane containing Cd (II) and Ni (II) that was already complexed by TSILs in 60OC at pH 6.0-7.5. After optimized conditions, the enrichment factor (EF), Linear range (LR) and limit of detection (LOD) were obtained (19.3; 19.6), (5.0- 415 μg L-1; 2.7- 92 μg L-1) and (1.3 μg L-1;  0.6 μg L-1) with [CHCA] [DEA] and (13.7; 14.2), (7.5- 600 μg L-1; 3.6- 128 μg L-1) and (2.2 ng L-1; 0.9 μg L-1) with [HEMIM][BF4] for Ni and Cd ions in olive  samples respectively.

scholarly journals Identification of Lymphatic and Hematogenous Routes of Rapidly Labeled Radioactive and Fluorescent Exosomes through Highly Sensitive Multimodal Imaging

2020 ◽  
Vol 21 (21) ◽  
pp. 7850
Author(s):  
Kyung Oh Jung ◽  
Young-Hwa Kim ◽  
Seock-Jin Chung ◽  
Chul-Hee Lee ◽  
Siyeon Rhee ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Optical Imaging ◽  
Ex Vivo ◽  
Hematogenous Metastasis ◽  
Positron Emission ◽  
Highly Sensitive ◽  
Mouse Breast Cancer ◽  
Rapid Labeling ◽  
Labeling Method

There has been considerable interest in the clinical use of exosomes as delivery vehicles for treatments as well as for promising diagnostic biomarkers, but the physiological distribution of exosomes must be further elucidated to validate their efficacy and safety. Here, we aimed to develop novel methods to monitor exosome biodistribution in vivo using positron emission tomography (PET) and optical imaging. Exosomes were isolated from cultured mouse breast cancer cells and labeled for PET and optical imaging. In mice, radiolabeled and fluorescently labeled exosomes were injected both via lymphatic and hematogenous metastatic routes. PET and fluorescence images were obtained and quantified. Radioactivity and fluorescence intensity of ex vivo organs were measured. PET signals from exosomes in the lymphatic metastatic route were observed in the draining sentinel lymph nodes. Immunohistochemistry revealed greater exosome uptake in brachial and axillary versus inguinal lymph nodes. Following administration through the hematogenous metastasis pathway, accumulation of exosomes was clearly observed in the lungs, liver, and spleen. Exosomes from tumor cells were successfully labeled with 64Cu (or 68Ga) and fluorescence and were visualized via PET and optical imaging, suggesting that this simultaneous and rapid labeling method could provide valuable information for further exosome translational research and clinical applications.

scholarly journals Modification of EDC method for increased labeling efficiency and characterization of low-content protein in gum acacia using asymmetrical flow field-flow fractionation coupled with multiple detectors

2021 ◽  
Author(s):  
Meiyu Zhang ◽  
Lars Nilsson ◽  
Seungho Lee ◽  
Jaeyeong Choi
Keyword(s):  
Flow Field ◽  
Fluorescence Spectroscopy ◽  
Molar Mass ◽  
Arabinogalactan Protein ◽  
Fluorescence Labeling ◽  
Field Flow Fractionation ◽  
Gum Acacia ◽  
Highly Sensitive ◽  
Flow Fractionation ◽  
Labeling Method

Abstract1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) is widely used as a crosslinker for fluorescence labeling of protein in the fields of biochemistry and food analysis. Many natural polysaccharides often contain some proteins or peptides that are very low in content but play a vital role in their biological function as well as technical applications. Determination of these low-content proteinaceous matters requires a highly sensitive and selective method. In this study, a methodological approach for investigations of the presence of proteinaceous material over the molar mass distribution (MD) of polysaccharides was developed using gum acacia (GA) as a model polysaccharide. EDC fluorescence-labeling method was modified by changing the pH (7, 9, and 11) of the solution for the analysis of low-content protein in food materials. Fluorescence spectroscopy and asymmetrical flow field-flow fractionation (AF4) were employed for characterizing the labeling efficiency and physiochemical properties of unlabeled and fluorescence-labeled GA. AF4 provided molar mass (M) and the radius of gyration (rG) of arabinogalactan (AG) and arabinogalactan protein complex (AGP) and determined the presence of proteinaceous matter over the MD. The labeling efficiencies of GA at pH 7, 9, and 11 determined by fluorescence spectroscopy were 56.5, 68.4, and 72.0%, respectively, with an increment of 15.5% when pH was increased from 7 to 11. The modified EDC fluorescence-labeling method allows highly sensitive and selective analysis of low-content proteinaceous matters and their distribution in natural polysaccharides. Graphical abstract

Ovarian Dysfunction in Fetally Irradiated Beagles

1977 ◽  
Vol 35 ◽  
pp. 658-659
Author(s):  
T. M. Seed ◽  
M. H. Sanderson ◽  
D. L. Gutzeit ◽  
T. E. Fritz ◽  
D. V. Tolle ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Low Dose ◽  
Long Term Effects ◽  
Ovarian Dysfunction ◽  
Dose Rates ◽  
Highly Sensitive ◽  
Microscopic Evaluation ◽  
Ovarian Pathology ◽  
Normal Offspring

The developing mammalian fetus is thought to be highly sensitive to ionizing radiation. However, dose, dose-rate relationships are not well established, especially the long term effects of protracted, low-dose exposure. A previous report (1) has indicated that bred beagle bitches exposed to daily doses of 5 to 35 R 60Co gamma rays throughout gestation can produce viable, seemingly normal offspring. Puppies irradiated in utero are distinguishable from controls only by their smaller size, dental abnormalities, and, in adulthood, by their inability to bear young.We report here our preliminary microscopic evaluation of ovarian pathology in young pups continuously irradiated throughout gestation at daily (22 h/day) dose rates of either 0.4, 1.0, 2.5, or 5.0 R/day of gamma rays from an attenuated 60Co source. Pups from non-irradiated bitches served as controls. Experimental animals were evaluated clinically and hematologically (control + 5.0 R/day pups) at regular intervals.

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