scholarly journals Tracking the Emergence of High Affinity Aptamers for rhVEGF165 During Capillary Electrophoresis-Systematic Evolution of Ligands by Exponential Enrichment Using High Throughput Sequencing

2013 ◽  
Vol 85 (22) ◽  
pp. 10761-10770 ◽  
Author(s):  
Meng Jing ◽  
Michael T. Bowser
Keyword(s):  
Capillary Electrophoresis ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
High Affinity ◽  
Systematic Evolution ◽  
Exponential Enrichment

scholarly journals Implementation of High-Throughput Sequencing (HTS) in Aptamer Selection Technology

2020 ◽  
Vol 21 (22) ◽  
pp. 8774
Author(s):  
Natalia Komarova ◽  
Daria Barkova ◽  
Alexander Kuznetsov
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Combinatorial Libraries ◽  
Structure And Function ◽  
Huge Number ◽  
Systematic Evolution ◽  
And Function ◽  
Exponential Enrichment

Aptamers are nucleic acid ligands that bind specifically to a target of interest. Aptamers have gained in popularity due to their high potential for different applications in analysis, diagnostics, and therapeutics. The procedure called systematic evolution of ligands by exponential enrichment (SELEX) is used for aptamer isolation from large nucleic acid combinatorial libraries. The huge number of unique sequences implemented in the in vitro evolution in the SELEX process imposes the necessity of performing extensive sequencing of the selected nucleic acid pools. High-throughput sequencing (HTS) meets this demand of SELEX. Analysis of the data obtained from sequencing of the libraries produced during and after aptamer isolation provides an informative basis for precise aptamer identification and for examining the structure and function of nucleic acid ligands. This review discusses the technical aspects and the potential of the integration of HTS with SELEX.

scholarly journals Identifying high-affinity aptamer ligands with defined cross-reactivity using high-throughput guided systematic evolution of ligands by exponential enrichment

2015 ◽  
Vol 43 (12) ◽  
pp. e82-e82 ◽  
Author(s):  
Agata Levay ◽  
Randall Brenneman ◽  
Jan Hoinka ◽  
David Sant ◽  
Marco Cardone ◽  
...  
Keyword(s):  
High Throughput ◽  
Cross Reactivity ◽  
High Affinity ◽  
Systematic Evolution ◽  
Exponential Enrichment

scholarly journals Aptamer-iRNAs as Therapeutics for Cancer Treatment

2018 ◽  
Vol 11 (4) ◽  
pp. 108 ◽  
Author(s):  
Mario Soldevilla ◽  
Daniel Meraviglia-Crivelli de Caso ◽  
Ashwathi Menon ◽  
Fernando Pastor
Keyword(s):  
Cancer Therapy ◽  
Cancer Treatment ◽  
Antisense Oligonucleotides ◽  
Tertiary Structure ◽  
Potential Treatment ◽  
High Affinity ◽  
Different Types ◽  
Systematic Evolution ◽  
Exponential Enrichment ◽  
Selection Of

Aptamers are single-stranded oligonucleotides (ssDNA or ssRNA) that bind and recognize their targets with high affinity and specificity due to their complex tertiary structure. Aptamers are selected by a method called SELEX (Systematic Evolution of Ligands by EXponential enrichment). This method has allowed the selection of aptamers to different types of molecules. Since then, many aptamers have been described for the potential treatment of several diseases including cancer. It has been described over the last few years that aptamers represent a very useful tool as therapeutics, especially for cancer therapy. Aptamers, thanks to their intrinsic oligonucleotide nature, present inherent advantages over other molecules, such as cell-based products. Owing to their higher tissue penetrability, safer profile, and targeting capacity, aptamers are likely to become a novel platform for the delivery of many different types of therapeutic cargos. Here we focus the review on interfering RNAs (iRNAs) as aptamer-based targeting delivered agents. We have gathered the most reliable information on aptamers as targeting and carrier agents for the specific delivery of siRNAs, shRNA, microRNAs, and antisense oligonucleotides (ASOs) published in the last few years in the context of cancer therapy.

scholarly journals Bioapplications of Cell-SELEX-Generated Aptamers in Cancer Diagnostics, Therapeutics, Theranostics and Biomarker Discovery: A Comprehensive Review

Cancers ◽  
2018 ◽  
Vol 10 (2) ◽  
pp. 47 ◽  
Author(s):  
Xuehui Pang ◽  
Cheng Cui ◽  
Shuo Wan ◽  
Ying Jiang ◽  
Liangliang Zhang ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
Drug Carriers ◽  
Cancer Diagnostics ◽  
High Affinity ◽  
Protein Receptors ◽  
Target Molecules ◽  
Systematic Evolution ◽  
Multifunctional Molecules ◽  
Exponential Enrichment ◽  
Single Stranded Oligonucleotide

Currently, functional single-stranded oligonucleotide probes, termed aptamers, generated by an iterative technology, Systematic Evolution of Ligands by Exponential Enrichment (SELEX), are utilized to selectively target molecules or cells with high affinity. Aptamers hold considerable promise as multifunctional molecules or conjugates for challenging nanotechnologies or bioapplications now and in the future. In this review, we first describe recent endeavors to select aptamers towards live cancer cells via cell-SELEX. We then introduce several characteristic applications of selected aptamers, especially in imaging, drug delivery and therapy. In part, these advances have been made possible via synthesis of aptamer-based nanomaterials, which, by their sizes, shapes, and physicochemical properties, allow such aptamer-nanomaterial complexes to function as signal reporters or drug carriers. We also describe how these aptamer-based molecular tools contribute to cancer biomarker discovery through high-affinity recognition of membrane protein receptors.

scholarly journals Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Rui-Yun Tian ◽  
Chao Lin ◽  
Shi-Yu Yu ◽  
Sheng Gong ◽  
Pan Hu ◽  
...  
Keyword(s):  
Research Direction ◽  
High Affinity ◽  
Complex Processes ◽  
Ssdna Aptamer ◽  
Coefficient Corrélation ◽  
Recognition Probe ◽  
Detection Probe ◽  
Systematic Evolution ◽  
Alternative Detection ◽  
Exponential Enrichment

The existing assays for detecting brevetoxin (BTX) depend on expensive equipment with a professional operator or on an antibody with limited stability, which requires complex processes, a high cost, and a considerable amount of time. The development of an alternative detection probe is another promising research direction. This paper reports the use of aptamers binding to BTX-2 in an analytical assay using the systematic evolution of ligands by exponential enrichment (SELEX). After 12 rounds of selection, the secondary structures of 25 sequences were predicted. Compared to other aptamers, Bap5 has relatively high affinity with the lowest dissociation constant of 4.83 μM, and IC50is 73.81 ng mL−1. A good linear regression formula ofy=30.688x-7.329with a coefficient correlation ofR2= 0.9798 was obtained using a biotin-avidin ELISA. Moreover, there is no cross-reaction with the detected marine toxins, except for BTX-2. Thus, Bap5 has potential to detect BTX-2 in shellfish in the future as a substitute for the recognition probe.

scholarly journals Specific inhibition of FGF5-induced cell proliferation by RNA aptamers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ryo Amano ◽  
Masato Namekata ◽  
Masataka Horiuchi ◽  
Minami Saso ◽  
Takuya Yanagisawa ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Surface Plasmon Resonance ◽  
Growth Factor ◽  
Hair Growth ◽  
Rna Aptamers ◽  
Specific Inhibition ◽  
Fibroblast Growth ◽  
High Affinity ◽  
Systematic Evolution ◽  
Exponential Enrichment

AbstractFibroblast growth factor 5 (FGF5) is a crucial regulator of hair growth and an oncogenic factor in several human cancers. To generate FGF5 inhibitors, we performed Systematic Evolution of Ligands by EXponential enrichment and obtained novel RNA aptamers that have high affinity to human FGF5. These aptamers inhibited FGF5-induced cell proliferation, but did not inhibit FGF2-induced cell proliferation. Surface plasmon resonance demonstrated that one of the aptamers, F5f1, binds to FGF5 tightly (Kd = 0.7 ± 0.2 nM), but did not fully to FGF1, FGF2, FGF4, FGF6, or FGFR1. Based on sequence and secondary structure similarities of the aptamers, we generated the truncated aptamer, F5f1_56, which has higher affinity (Kd = 0.118 ± 0.003 nM) than the original F5f1. Since the aptamers have high affinity and specificity to FGF5 and inhibit FGF5-induced cell proliferation, they may be candidates for therapeutic use with FGF5-related diseases or hair disorders.

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