scholarly journals High Lateral Resolution vs Molecular Preservation in near-IR fs-Laser Desorption Postionization Mass Spectrometry

2014 ◽  
Vol 87 (1) ◽  
pp. 367-371 ◽  
Author(s):  
Yang Cui ◽  
Igor V. Veryovkin ◽  
Michael W. Majeski ◽  
Daniel R. Cavazos ◽  
Luke Hanley
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Lateral Resolution ◽  
Near Ir ◽  
High Lateral Resolution ◽  
Fs Laser

Mass spectrometry imaging of thin-layer chromatography plates using laser desorption/low-temperature plasma ionisation

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3885-3891
Author(s):  
Nancy Shyrley García-Rojas ◽  
Abigail Moreno-Pedraza ◽  
Ignacio Rosas-Román ◽  
Enrique Ramírez-Chávez ◽  
Jorge Molina-Torres ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Low Temperature ◽  
Mass Spectrometry Imaging ◽  
Laser Desorption ◽  
Low Temperature Plasma ◽  
Temperature Plasma ◽  
High Lateral Resolution ◽  
Open Development ◽  
Tlc Plates ◽  
Layer Chromatography

An open development kit for ambient ionisation enables the fast scanning and visualisation of TLC plates with high lateral resolution.

Venom Gland Mass Spectrometry Imaging of Saw-Scaled Viper, Echis carinatus sochureki, at High Lateral Resolution

2021 ◽  
Author(s):  
Parviz Ghezellou ◽  
Sven Heiles ◽  
Patrik Kadesch ◽  
Alireza Ghassempour ◽  
Bernhard Spengler
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Venom Gland ◽  
Lateral Resolution ◽  
High Lateral Resolution ◽  
Echis Carinatus

scholarly journals Multifunctional Reactive MALDI Matrix Enabling High-Lateral Resolution Dual Polarity MSI and Lipid C=C Position-Resolved MS2I

2020 ◽  
Author(s):  
Fabian Wäldchen ◽  
Franziska Mohr ◽  
Andreas H. Wagner ◽  
Sven Heiles
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Negative Ion ◽  
Lateral Resolution ◽  
Maldi Matrix ◽  
Spatially Resolved ◽  
High Lateral Resolution ◽  
Dual Polarity ◽  
Negative Ion Mode

Local lipid variations in tissues are readily revealed with mass spectrometry imaging (MSI) methods and resulting lipid distributions serve as bioanalytical signatures to reveal cell- or tissue-specific lipids. Comprehensive MSI lipid mapping requires measurements in both ion polarities. Additionally, structural lipid characterization is necessary to link lipid structure to lipid function. Whereas some structural elements of lipids are readily derived from high-resolution mass spectrometry (MS) and tandem-MS (MSn), the localization of C=C double bonds (DBs) requires specialized fragmentation and/or functionalization methods. In this work, we identify a multifunctional matrix-assisted laser desorption/ionization (MALDI) matrix for spatially-resolved lipidomics investigations that reacts with lipids in Paternò-Büchi (PB) reactions during laser irradiation facilitating DB position assignment and allows dual polarity high-resolution MALDI-MSI and MALDI MS2I studies. By screening twelve compounds for improved ionization efficiency in positive/negative ion mode and PB functionalization yield compared to the previously introduced reactive MALDI matrix benzophenone, benzoylpyridine (BzPy) is identified as the best candidate. The multifunctional character of the new matrix enables DB localization of authentic standards belonging to twelve lipid classes and helps to assign 506/365 lipid features in positive/negative ion mode from mouse cerebellum tissue. The analytical capabilities of BzBy as a multifunctional MALDI-MSI matrix are demonstrated by imaging endogenous and PB-functionalized lipids in mouse kidney sections with 7 µm lateral resolution in both ion modes. Tracking diagnostic lipid DB position fragment ions in mouse pancreas tissue with down to 10 µm pixel size allows to identify islets of Langerhans associated lipid isomer upregulation or depletion. <br>

Multifunctional Reactive MALDI Matrix Enabling High-Lateral Resolution Dual Polarity MSI and Lipid C=C Position-Resolved MS2I

2020 ◽  
Author(s):  
Fabian Wäldchen ◽  
Franziska Mohr ◽  
Andreas H. Wagner ◽  
Sven Heiles
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Negative Ion ◽  
Lateral Resolution ◽  
Maldi Matrix ◽  
Spatially Resolved ◽  
High Lateral Resolution ◽  
Dual Polarity ◽  
Negative Ion Mode

Local lipid variations in tissues are readily revealed with mass spectrometry imaging (MSI) methods and resulting lipid distributions serve as bioanalytical signatures to reveal cell- or tissue-specific lipids. Comprehensive MSI lipid mapping requires measurements in both ion polarities. Additionally, structural lipid characterization is necessary to link lipid structure to lipid function. Whereas some structural elements of lipids are readily derived from high-resolution mass spectrometry (MS) and tandem-MS (MSn), the localization of C=C double bonds (DBs) requires specialized fragmentation and/or functionalization methods. In this work, we identify a multifunctional matrix-assisted laser desorption/ionization (MALDI) matrix for spatially-resolved lipidomics investigations that reacts with lipids in Paternò-Büchi (PB) reactions during laser irradiation facilitating DB position assignment and allows dual polarity high-resolution MALDI-MSI and MALDI MS2I studies. By screening twelve compounds for improved ionization efficiency in positive/negative ion mode and PB functionalization yield compared to the previously introduced reactive MALDI matrix benzophenone, benzoylpyridine (BzPy) is identified as the best candidate. The multifunctional character of the new matrix enables DB localization of authentic standards belonging to twelve lipid classes and helps to assign 506/365 lipid features in positive/negative ion mode from mouse cerebellum tissue. The analytical capabilities of BzBy as a multifunctional MALDI-MSI matrix are demonstrated by imaging endogenous and PB-functionalized lipids in mouse kidney sections with 7 µm lateral resolution in both ion modes. Tracking diagnostic lipid DB position fragment ions in mouse pancreas tissue with down to 10 µm pixel size allows to identify islets of Langerhans associated lipid isomer upregulation or depletion. <br>

scholarly journals Supported Membrane Composition Analysis by Secondary Ion Mass Spectrometry with High Lateral Resolution

2005 ◽  
Vol 88 (4) ◽  
pp. 2965-2975 ◽  
Author(s):  
Carine Galli Marxer ◽  
Mary L. Kraft ◽  
Peter K. Weber ◽  
Ian D. Hutcheon ◽  
Steven G. Boxer
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Lateral Resolution ◽  
Composition Analysis ◽  
Membrane Composition ◽  
Supported Membrane ◽  
High Lateral Resolution ◽  
Ion Mass Spectrometry ◽  
Secondary Ion
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