Universal and Programmable Rolling Circle Amplification-CRISPR/Cas12a-Mediated Immobilization-Free Electrochemical Biosensor

2021 ◽  
Author(s):  
Min Qing ◽  
Sheng Liang Chen ◽  
Zhe Sun ◽  
Yi Fan ◽  
Hong Qun Luo ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Rolling Circle

scholarly journals Rapid electrochemical detection of coronavirus SARS-CoV-2

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Thanyarat Chaibun ◽  
Jiratchaya Puenpa ◽  
Tatchanun Ngamdee ◽  
Nimaradee Boonapatcharoen ◽  
Pornpat Athamanolap ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Sandwich Hybridization ◽  
Redox Active ◽  
One Step ◽  
The One

AbstractCoronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

Highly Sensitive Electrochemical Biosensor for Circulating Tumor Cells Detection via Dual-Aptamer Capture and Rolling Circle Amplification Strategy

2019 ◽  
Vol 15 (7) ◽  
pp. 1568-1577 ◽  
Author(s):  
Yang Wang ◽  
Kai Chang ◽  
Cheng Yang ◽  
Shujing Li ◽  
Lixin Wang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Metastasis ◽  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
K562 Cells ◽  
Detection Sensitivity ◽  
Experimental Conditions ◽  
Simple Method ◽  
Rolling Circle

A fast and simple strategy for early detection of circulating tumor cells (CTCs) is urgently required because of cancer metastasis. In this work, we assembled an electrochemical biosensor by two aptamers that could form hairpin and specifically recognize K562 cells. The thiolated capture aptamer was fixed on the gold electrode surface. The detection aptamer was linked with a primer at 3 end which could trigger rolling circle amplification to prolong the sequence of aptamer. The dual-aptamer model was fabricated to improve the capture specificity and efficiency for K562 cells. The rolling circle amplification improved the detection sensitivity by inhibiting electron transfer of [Fe(CN)6]3–/4– which could be measured by differential pulse voltammetry. The detection limit of 25 cells mL–1 and linear ranges of 1 × 10 2 to 1 × 105 cells mL–1 were obtained under optimal experimental conditions. Our work exhibited a label-free and simple method for detecting CTCs using cell-specific aptasensor, showing an expected possibility for further CTCs-related study and clinical applications of this novel method.

Rolling circle amplification in electrochemical biosensor with biomedical applications

2016 ◽  
Vol 781 ◽  
pp. 223-232 ◽  
Author(s):  
Chang Feng ◽  
Xiaoxia Mao ◽  
Yucai Yang ◽  
Xiaoli Zhu ◽  
Yongmei Yin ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Rolling Circle

A ratiometric electrochemical biosensor for ultrasensitive and highly selective detection of the K-ras gene via exonuclease III-assisted target recycling and rolling circle amplification strategies

2019 ◽  
Vol 11 (32) ◽  
pp. 4146-4156 ◽  
Author(s):  
Qi Xiao ◽  
Jinrong Feng ◽  
Jiawen Li ◽  
Yi Liu ◽  
Dan Wang ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Ras Gene ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Rolling Circle ◽  
Exo Iii

A ratiometric electrochemical biosensor for ultrasensitive and highly selective detection of the K-ras gene via Exo III-assisted target recycling and RCA strategies.

An ultrasensitive electrochemical biosensor for Pseudomonas aeruginosa assay based on a rolling circle amplification-assisted multipedal DNA walker

2020 ◽  
Vol 56 (46) ◽  
pp. 6273-6276 ◽  
Author(s):  
Huang Zhou ◽  
Shaoyun Duan ◽  
Ji Huang ◽  
Fengjiao He
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Disease Diagnosis ◽  
Rrna Gene ◽  
Rolling Circle ◽  
Dna Walker ◽  
Application Potential

An ultrasensitive electrochemical biosensor was developed based on RCA and multipedal DNA walking strategy for the assay of 16S rRNA gene, and it has great application potential in food safety, environmental monitoring, and disease diagnosis.

scholarly journals Rapid Electrochemical Detection of Coronavirus SARS-CoV-2.

2020 ◽  
Author(s):  
Thanyarat Chaibun ◽  
Jiratchaya Puenpa ◽  
Tatchanun Ngamdee ◽  
Nimaradee Boonapatcharoen ◽  
Pornpat Athamanolap ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Sandwich Hybridization ◽  
Redox Active ◽  
One Step ◽  
The One

Abstract COVID-19 is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/mL of N and S genes, in less than 2 hours. Sensor evaluation with 105 clinical samples, including 40 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

scholarly journals A sensitive electrochemical assay for T4 polynucleotide kinase activity based on titanium dioxide nanotubes and a rolling circle amplification strategy

RSC Advances ◽  
2018 ◽  
Vol 8 (67) ◽  
pp. 38436-38444 ◽  
Author(s):  
Yanli Zhang ◽  
Xiang Fang ◽  
Zhenyu Zhu ◽  
Yanqiong Lai ◽  
Chunli Xu ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Kinase Activity ◽  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Electrochemical Assay ◽  
Titanium Dioxide Nanotubes ◽  
Rolling Circle ◽  
Polynucleotide Kinase ◽  
Amplification Strategy ◽  
T4 Polynucleotide Kinase

An ultrasensitive electrochemical biosensor was developed for detection of T4 polynucleotide kinase activity based on titanium dioxide nanotubes and a rolling circle amplification strategy.

Sign in / Sign up

Export Citation Format

Share Document