Method for Sorting and Pairwise Selection of Nanobodies for the Development of Highly Sensitive Sandwich Immunoassays

Sahelian soils are recognized for their low N content, but their mineral N concentrations can often be high enough to inhibit N2 fixation in cultivated legumes. However, the quest for symbiotic plant genotypes tolerant of mineral N is poorly documented for nitrogen-fixing trees. The behaviour of N2 fixing symbioses with regard to nitrogen fertilization was observed in different Faidherbia albida (Del) A. Chev. genotypes. Twelve distinct lineages of the species, obtained from four regions of western Africa, were cultivated for 75 days in the presence of 06 mol·m3 N provided as NH4NO3. Plants were separately inoculated with two Bradyrhizobium strains, and cultivated on sandy soil in a nursery. Aerial and root parts lengths and masses, collar diameters, and ratios of height to length (H/P) of taproot, and aerial to root (MA/MR) parts masses increased with increased N level. Plants showed significant growth differences linked with seed origin, and with lineage for a same origin. Genotypical variations were observed for concentrations as well as C and N total uptakes, C/N ratio measured in aerial parts, and N2 fixation. Nodule number and mass were greatly reduced in the presence of 2 mol·m3 N. The extent of this reduction was dependent, to a lesser degree, on seed origin and Bradyrhizobium strain. Data show that F. albida Bradyrhizobium symbiosis is highly sensitive to mineral N. It is suggested that selection of symbiotic partners for tolerance to mineral N might greatly improve the agronomic behaviour of F. albida.Key words: Faidherbia albida, lineages, Bradyrhizobium, symbiosis, nodulation, growth, ammonium nitrate.

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Selection of DNA aptamers and establishment of an effective aptasensor for highly sensitive detection of cefquinome residues in milk

The Analyst ◽

10.1039/c8an00709h ◽

2018 ◽

Vol 143 (13) ◽

pp. 3202-3208 ◽

Cited By ~ 7

Author(s):

Lihui Wang ◽

Chuchu Wang ◽

Hao Li

Keyword(s):

Respiratory Infection ◽

Bovine Mastitis ◽

Generation Cephalosporin ◽

Sensitive Detection ◽

Fourth Generation ◽

Dna Aptamers ◽

Highly Sensitive ◽

Highly Sensitive Detection ◽

Selection Of

Cefquinome (CFQ), which is a fourth-generation cephalosporin approved for veterinary use only, has been widely used for treating porcine or bovine respiratory infection, bovine mastitis and other diseases.

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Dwarf Galaxies and Cluster Environments

Proceedings of the International Astronomical Union ◽

10.1017/s1743921318006890 ◽

2018 ◽

Vol 14 (S344) ◽

pp. 373-376

Author(s):

Yasuhiro Hashimoto ◽

J. Patrick Henry ◽

Hans Böhringer

Keyword(s):

Dwarf Galaxies ◽

Dwarf Galaxy ◽

Detection Algorithm ◽

Diffuse Light ◽

X Ray ◽

Optical Images ◽

Highly Sensitive ◽

The Galaxy ◽

Galaxy Populations ◽

Selection Of

AbstractWe report an investigation of the properties of dwarf galaxies (Mr < -15) inside 26 clusters at z = 0.15 – 0.25, using the X-ray data from the Chandra archive, and optical images taken with Subaru Suprime-Cam. Our results include: 1. Investigation of the dwarf galaxy density distribution is sensitive to the background galaxies and the choice of colour selection of galaxies. 2. Cluster-centric dwarf-to-giant ratio is highly sensitive to the level of subtracted background galaxies. 3. A certain fraction of faint galaxies always remain undetected by the detection algorithm near the center of clusters, even after carefully treating the halo or extra diffuse light created by bright galaxies. The number of ‘undetected’ faint galaxies varies significantly from cluster to cluster, and even from pointing to pointing. 4. Dwarf galaxies extend up to 2 Mpc from the center in most clusters. Meanwhile, the distribution of blue dwarf galaxies extends more to the outside. 5. For a given colour, the spatial distributions of dwarf galaxies and giant galaxies become similar. Namely, the most of the radial distribution comes from the colour, rather than the size, of galaxies. 6. Relative to the NFW profile, all of the galaxy populations are showing a deficit near the cluster core (r < 0.3 Mpc). 7. The dwarf-to-giant ratio shows no variation against cluster measures such as the richness and X-ray luminosity, as well as various cluster X-ray characteristics related to possible dynamical status of clusters.

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A methodological concept for material selection of highly sensitive components based on multiple criteria decision analysis

Expert Systems with Applications ◽

10.1016/j.eswa.2007.11.052 ◽

2009 ◽

Vol 36 (2) ◽

pp. 1362-1370 ◽

Cited By ~ 62

Author(s):

A. Shanian ◽

O. Savadogo

Keyword(s):

Decision Analysis ◽

Material Selection ◽

Multiple Criteria ◽

Multiple Criteria Decision Analysis ◽

Highly Sensitive ◽

Selection Of

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PEIS: a novel approach of tumor purity estimation by identifying information sites through integrating signal based on DNA methylation data

BMC Bioinformatics ◽

10.1186/s12859-019-3227-1 ◽

2019 ◽

Vol 20 (S22) ◽

Author(s):

Shudong Wang ◽

Lihua Wang ◽

Yuanyuan Zhang ◽

Shanchen Pang ◽

Xinzeng Wang

Keyword(s):

Dna Methylation ◽

Strong Correlation ◽

Estimation Methods ◽

Methylation Data ◽

Tumor Purity ◽

Novel Approach ◽

Highly Sensitive ◽

Number Variation ◽

High Consistency ◽

Selection Of

Abstract Background Tumor purity plays an important role in understanding the pathogenic mechanism of tumors. The purity of tumor samples is highly sensitive to tumor heterogeneity. Due to Intratumoral heterogeneity of genetic and epigenetic data, it is suitable to study the purity of tumors. Among them, there are many purity estimation methods based on copy number variation, gene expression and other data, while few use DNA methylation data and often based on selected information sites. Consequently, how to choose methylation sites as information sites has an important influence on the purity estimation results. At present, the selection of information sites was often based on the differentially methylated sites that only consider the mean signal, without considering other possible signals and the strong correlation among adjacent sites. Results Considering integrating multi-signals and strong correlation among adjacent sites, we propose an approach, PEIS, to estimate the purity of tumor samples by selecting informative differential methylation sites. Application to 12 publicly available tumor datasets, it is shown that PEIS provides accurate results in the estimation of tumor purity which has a high consistency with other existing methods. Also, through comparing the results of different information sites selection methods in the evaluation of tumor purity, it shows the PEIS is superior to other methods. Conclusions A new method to estimate the purity of tumor samples is proposed. This approach integrates multi-signals of the CpG sites and the correlation between the sites. Experimental analysis shows that this method is in good agreement with other existing methods for estimating tumor purity.

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Sensitivity to herbicides of two spring forage pea cultivars

Agricultural Science and Technology ◽

10.15547/ast.2019.03.040 ◽

2019 ◽

Vol 11 (3) ◽

pp. 232-235

Author(s):

S. Ivanov ◽

M. Dimitrova

Keyword(s):

Seed Mass ◽

Double Dose ◽

Average Value ◽

Highly Sensitive ◽

New Varieties ◽

Average Plant ◽

Factor C ◽

The Village ◽

Parcel Method ◽

Selection Of

Abstract. An important element of spring pea growing technology is weed control as the crop is highly sensitive to them, especially in the early stages of its development. The use of various herbicide preparations with a diverse mechanism and spectrum of action, changes in weed associations under the influence of various factors, as well as the selection of new varieties necessitates a constant study of the problem of the efficacy of herbicide preparations and the sensitivity of varieties to them. The aim of the present study was to investigate the sensitivity of two cultivars of spring forage pea to different herbicide preparations. During the period 2006-2008 in the village of Lyubenova mahala, Stara Zagora region, a three-factor field experiment was conducted by the fractional parcel method in four replications and size of the harvest plot 10m2. Factor A includes cultivars of spring peas, factor B – herbicide variants, factor C – doses of herbicides. It has been proven that the plants of both varieties, Pickardi and Amitie, are killed by 2.4 D, both in single and double dose. The highest average plant height was recorded in the Amitie cultivar treated with Basagran 600 SL herbicide at the double dose (3.0 L/ha). The highest were the values of the parameter number of beans per plant obtained in the Amitie cultivar treated with Basagran 600 SL and Pivot 100 SL – 0.8 L/ha, in the soil. A statistically proven highest average value of the parameter seed mass per plant was obtained in the Amitie variety treated with the Basagran 600 SL herbicide (12.667g) and the lowest – in Pickardi with the herbicide Pivot (10.330g).

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Prospektív vizsgálat a sigmoideoscopia diagnosztikai érzékenységének meghatározására vastagbelet infiltráló endometriosisban

Orvosi Hetilap ◽

10.1556/650.2017.30663 ◽

2017 ◽

Vol 158 (7) ◽

pp. 264-269

Author(s):

Péter Lukovich ◽

Noémi Csibi ◽

Réka Brubel ◽

Krisztina Tari ◽

Szilvia Csuka ◽

...

Keyword(s):

Laparoscopic Resection ◽

Correct Selection ◽

Gynaecological Surgery ◽

Sigmoidoscopic Examination ◽

Bowel Endometriosis ◽

Colorectal Endometriosis ◽

Highly Sensitive ◽

Secondary Signs ◽

Selection Of

Abstract: Introduction and aim: In the treatment of colorectal endometriosis a multidisciplinary laparoscopic resection is suggested, for this reason the correct selection of bowel infiltration is essential before surgery. Patients and method: Between 2009 and 2015, 383 sigmoidoscopies were performed in patients with endometriosis. Where mucosal invasion was absent secondary signs (wall rigidity, impression, kinking, pain during the examination, suffusion) were analysed. In endoscopically confirmed cases multidisciplinary surgery was performed, the remaining patients were operated by a gynecologic team only. Results: Endometriosis was endoscopically confirmed in 224 patients (58.49%), 108 of them underwent multidisciplinary operation, the negative 135 cases received gynaecological surgery. Bowel endometriosis was confirmed in 103 out of 108 cases intraoperatively, while in 8 cases of the sigmoidoscopically negative patients bowel infiltration was diagnosed intraoperatively by the gynaecological team. Complete sigmoidoscopy was performed in 43.47% of the cases. Intraluminal endometriosis was found in 4.91%, secondary signs as rigidity in 38.39%, impression in 45.54%, kinking in 57.14%, pain (in cases of examination without narcosis) in 26.06% and suffusion in 3.82% of the cases was found during sigmoidoscopy. Sigmoidoscopic examination has a 92.8% specificity and 96.2% sensitivity in cases of bowel endometriosis. Conclusion: Sigmoidoscopy performed by an experienced gastroenterologist is a highly sensitive examination for the diagnosis of bowel endometriosis. Orv. Hetil., 2017, 158(7), 264–269.

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Quantification of mtDNA in single oocytes, polar bodies and subcellular components by real-time rapid cycle fluorescence monitored PCR

Zygote ◽

10.1017/s0967199400001003 ◽

2000 ◽

Vol 8 (3) ◽

pp. 209-215 ◽

Cited By ~ 118

Author(s):

Nury Steuerwald ◽

Jason A. Barritt ◽

Rick Adler ◽

Henry Malter ◽

Timothy Schimmel ◽

...

Keyword(s):

Dna Amplification ◽

Human Oocyte ◽

Mtdna Copy Number ◽

Clinical Tool ◽

Physiological Processes ◽

Polar Bodies ◽

Highly Sensitive ◽

Mature Mouse ◽

Selection Of

Oocytes, in general, are greatly enriched in mitochondria to support higher rates of macromolecular synthesis and critical physiological processes characteristic of early development. An inability of these organelles to amplify and/or to accumulate ATP has been linked to developmental abnormality or arrest. The number of mitochondrial genomes present in mature mouse and human metaphase II oocytes was estimated by fluorescent rapid cycle DNA amplification, which is a highly sensitive technique ideally suited to quantitative mitochondrial DNA (mtDNA) analysis in individual cells. A considerable degree of variability was observed between individual samples. An overall average of 1.59 × 105 and 3.14 × 105 mtDNA molecules were detected per mouse and human oocyte, respectively. Furthermore, the mtDNA copy number was examined in polar bodies and contrasted with the concentration in their corresponding oocytes. In addition, the density of mtDNA in a cytoplasmic sample was estimated in an attempt to determine the approximate number of mitochondria transferred during clinical cytoplasmic donation procedures as well as to develop a clinical tool for the assessment and selection of oocytes during in vitro fertilisation procedures. However, no correlation was identified between the mtDNA concentration in either polar bodies or cytoplasmic samples and their corresponding oocyte.

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