DNA Probes for Implementation of Multiple Molecular Computations Using a Lateral Flow Strip Biosensor as the Sensing Platform

2018 ◽  
Vol 90 (17) ◽  
pp. 10311-10317 ◽  
Author(s):  
Junhua Chen ◽  
Shu Chen ◽  
Fengling Li
Keyword(s):  
Dna Probes ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Sensing Platform ◽  
Molecular Computations

scholarly journals Paper-Based Competitive Immunochromatography Coupled with an Enzyme-Modified Electrode to Enable the Wireless Monitoring and Electrochemical Sensing of Cotinine in Urine

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1659
Author(s):  
Nutcha Larpant ◽  
Pramod K. Kalambate ◽  
Tautgirdas Ruzgas ◽  
Wanida Laiwattanapaisal
Keyword(s):  
Glucose Oxidase ◽  
Modified Electrode ◽  
Near Field ◽  
Electrochemical Measurement ◽  
Electrochemical Sensing ◽  
Urine Samples ◽  
Lateral Flow Strip ◽  
Wireless Monitoring ◽  
Sensing Platform ◽  
Combined Strategy

This paper proposes a combined strategy of using paper-based competitive immunochromatography and a near field communication (NFC) tag for wireless cotinine determination. The glucose oxidase labeled cotinine antibody specifically binds free cotinine in a sample, whereas the unoccupied antibody attached to BSA-cotinine at the test line on a lateral flow strip. The glucose oxidase on the strip and an assistant pad in the presence of glucose generated H2O2 and imposed the Ag oxidation on the modified electrode. This enabled monitoring of immunoreaction by either electrochemical measurement or wireless detection. Wireless sensing was realized for cotinine in the range of 100–1000 ng/mL (R2 = 0.96) in PBS medium. Undiluted urine samples from non-smokers exhibited an Ag-oxidation rate three times higher than the smoker’s urine samples. For 1:8 diluted urine samples (smokers), the proposed paper-based competitive immunochromatography coupled with an enzyme-modified electrode differentiated positive and negative samples and exhibited cotinine discrimination at levels higher than 12 ng/mL. This novel sensing platform can potentially be combined with a smartphone as a reader unit.

scholarly journals Rational Programming of Cas12a for Early-Stage Detection of COVID-19 by Lateral Flow Assay and Portable Real-Time Fluorescence Readout Facilities

Biosensors ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 11
Author(s):  
Zhijian Yi ◽  
Jean de Dieu Habimana ◽  
Omar Mukama ◽  
Zhiyuan Li ◽  
Nelson Odiwuor ◽  
...  
Keyword(s):  
Real Time ◽  
Early Stage ◽  
Limited Resource ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Capture Probe ◽  
Fluorescence Detector ◽  
Lateral Flow Strip ◽  
Global Pandemic ◽  
Novel Approach

Coronavirus disease 2019 (COVID-19) caused by the SARS-CoV-2 virus has led to a global pandemic with a high spread rate and pathogenicity. Thus, with limited testing solutions, it is imperative to develop early-stage diagnostics for rapid and accurate detection of SARS-CoV-2 to contain the rapid transmission of the ongoing COVID-19 pandemic. In this regard, there remains little knowledge about the integration of the CRISPR collateral cleavage mechanism in the lateral flow assay and fluorophotometer. In the current study, we demonstrate a CRISPR/Cas12a-based collateral cleavage method for COVID-19 diagnosis using the Cas12a/crRNA complex for target recognition, reverse transcription loop-mediated isothermal amplification (RT-LAMP) for sensitivity enhancement, and a novel DNA capture probe-based lateral flow strip (LFS) or real-time fluorescence detector as the parallel system readout facility, termed CRICOLAP. Our novel approach uses a customized reporter that hybridizes an optimized complementary capture probe fixed at the test line for naked-eye result readout. The CRICOLAP system achieved ultra-sensitivity of 1 copy/µL in ~32 min by portable real-time fluorescence detection and ~60 min by LFS. Furthermore, CRICOLAP validation using 60 clinical nasopharyngeal samples previously verified with a commercial RT-PCR kit showed 97.5% and 100% sensitivity for S and N genes, respectively, and 100% specificity for both genes of SARS-CoV-2. CRICOLAP advances the CRISPR/Cas12a collateral cleavage result readout in the lateral flow assay and fluorophotometer, and it can be an alternative method for the decentralized field-deployable diagnosis of COVID-19 in remote and limited-resource locations.

scholarly journals Development and Performance verification of colloidal gold labeled SARS-CoV-2 antigen detection method for routine popular screening of COVID-19 with clinical samples in Poland and China

2021 ◽  
Author(s):  
Chuanxiang Guo ◽  
Li Yao ◽  
Fengling Chen ◽  
Chao Zhang ◽  
Wei Chen
Keyword(s):  
Predictive Value ◽  
Colloidal Gold ◽  
Detection Method ◽  
Age Distribution ◽  
Antigen Detection ◽  
Lateral Flow ◽  
Rapid Screening ◽  
Clinical Samples ◽  
Lateral Flow Strip ◽  
And Performance

In this research, we have constructed and optimized the colloidal gold labeled lateral flow strip (LFS) for rapid detection of antigen of SARS-CoV-2 and rapid screening of COVID-19. Based on the constructed and optimized colloidal gold lateral flow strip, the parameters of the LFS have been well evaluated with the clinical samples in the professional labs. The screening performance have also been evaluated from the aspects including the CT values, age distribution and onset of symptoms. Finally, based on the detection results of 420 clinical samples, the LFS can achieve the screening of COVID-19 with the positive percentage agreement (PPA, sensitivity), negative percent agreement (NPA, specificity), the positive predictive value (PPV) and the negative predictive value (NPV) of 96.8%, 100%, 100% and 96.6%, respectively, indicating the powerful potential for practical screening applications in pandemic control. Of great significance, this developed SARS-CoV-2 antigen detection method has also been successfully utilized for screening of delta-variant of SARS-CoV-2.

Potential of cross-priming amplification and DNA-based lateral-flow strip biosensor for rapid on-site GMO screening

2014 ◽  
Vol 406 (17) ◽  
pp. 4243-4249 ◽  
Author(s):  
Xin Huang ◽  
Congcong Zhai ◽  
Qimin You ◽  
Hongjun Chen
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Strip

Development of a dry-reagent-based nucleic acid-sensing platform by coupling thermostabilised LATE-PCR assay to an oligonucleotide-modified lateral flow biosensor

2015 ◽  
Vol 118 ◽  
pp. 99-105 ◽  
Author(s):  
Geik Yong Ang ◽  
Choo Yee Yu ◽  
Kok Gan Chan ◽  
Kirnpal Kaur Banga Singh ◽  
Yean Chan Yean
Keyword(s):  
Nucleic Acid ◽  
Lateral Flow ◽  
Pcr Assay ◽  
Sensing Platform
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