3D Bioprinting of Bone Marrow Mesenchymal Stem Cell-Laden Silk Fibroin Double Network Scaffolds for Cartilage Tissue Repair

2020 ◽  
Vol 31 (8) ◽  
pp. 1938-1947 ◽  
Author(s):  
Tianyu Ni ◽  
Min Liu ◽  
Yajie Zhang ◽  
Yi Cao ◽  
Renjun Pei
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Silk Fibroin ◽  
Tissue Repair ◽  
Cartilage Tissue ◽  
3D Bioprinting ◽  
Double Network

scholarly journals Human Umbilical Cord Mesenchymal Stem Cell Derived Exosomes Delivered Using Silk Fibroin and Sericin Composite Hydrogel Promote Wound Healing

2021 ◽  
Vol 8 ◽  
Author(s):  
Chaoshan Han ◽  
Feng Liu ◽  
Yu Zhang ◽  
Wenjie Chen ◽  
Wei Luo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
Silk Fibroin ◽  
Extraction Method ◽  
Tissue Repair ◽  
Composite Hydrogel ◽  
Human Umbilical Cord ◽  
Beneficial Effects

Recent studies have shown that the hydrogels formed by composite biomaterials are better choice than hydrogels formed by single biomaterial for tissue repair. We explored the feasibility of the composite hydrogel formed by silk fibroin (SF) and silk sericin (SS) in tissue repair for the excellent mechanical properties of SF, and cell adhesion and biocompatible properties of SS. In our study, the SF SS hydrogel was formed by SF and SS protein with separate extraction method (LiBr dissolution for SF and hot alkaline water dissolution for SS), while SF-SS hydrogel was formed by SF and SS protein using simultaneous extraction method (LiBr dissolution for SF and SS protein). The effects of the two composite hydrogels on the release of inflammatory cytokines from macrophages and the wound were analyzed. Moreover, two hydrogels were used to encapsulate and deliver human umbilical cord mesenchymal stem cell derived exosomes (UMSC-Exo). Both SF SS and SF-SS hydrogels promoted wound healing, angiogenesis, and reduced inflammation and TNF-α secretion by macrophages. These beneficial effects were more significant in the experimental group treated by UMSC-Exo encapsulated in SF-SS hydrogel. Our study found that SF-SS hydrogel could be used as an excellent alternative to deliver exosomes for tissue repair.

Feasibility of Autologous Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Matrix Scaffold for Cartilage Tissue Engineering

2013 ◽  
Vol 37 (12) ◽  
pp. E179-E190 ◽  
Author(s):  
Cheng Tang ◽  
Yan Xu ◽  
Chengzhe Jin ◽  
Byoung-Hyun Min ◽  
Zhiyong Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cartilage Tissue Engineering ◽  
Autologous Bone Marrow ◽  
Cartilage Tissue ◽  
Autologous Bone ◽  
Extracellular Matrix Scaffold

3D bioprinting mesenchymal stem cell-laden construct with core–shell nanospheres for cartilage tissue engineering

2018 ◽  
Vol 29 (18) ◽  
pp. 185101 ◽  
Author(s):  
Wei Zhu ◽  
Haitao Cui ◽  
Benchaa Boualam ◽  
Fahed Masood ◽  
Erin Flynn ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Core Shell ◽  
3D Bioprinting

Mesenchymal Stem Cell‐Derived Exosomes for Effective Cartilage Tissue Repair and Treatment of Osteoarthritis

2020 ◽  
Vol 15 (12) ◽  
pp. 2000082 ◽  
Author(s):  
Young Guk Kim ◽  
Jonghoon Choi ◽  
Kyobum Kim
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Tissue Repair ◽  
Cartilage Tissue

Polo Like Kinase 4 (PLK4) impairs human bone marrow mesenchymal stem cell (BMSC) viability and osteogenic differentiation

2021 ◽  
Vol 549 ◽  
pp. 221-228
Author(s):  
Bin Zhou ◽  
Kun Peng ◽  
Guoqiang Wang ◽  
Weihua Chen ◽  
Yijun Kang
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Human Bone ◽  
Human Bone Marrow

In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment

2021 ◽  
pp. 039139882110255
Author(s):  
Sara Anajafi ◽  
Azam Ranjbar ◽  
Monireh Torabi-Rahvar ◽  
Naser Ahmadbeigi
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cultured Cells ◽  
Morphological Evidence ◽  
Cell Aggregates ◽  
Plla Scaffold ◽  
Significant Difference

Background: Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods. Materials and methods: Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice. Result: Immunohistochemistry results indicated that there was a significant difference ( p < 0.050) in CD31+ cells between PLLA scaffolds contained cultured BM-MSC; PLLA scaffolds contained BM-aggregates and empty PLLA. According to morphological evidence, obvious connections with recipient vasculature and acceptable integration with surroundings were established in MSC and aggregate-seeded scaffolds. Conclusion: Our findings revealed cultured BM-MSC and BM-aggregates, capacity in order to develop numerous connections between PLLA scaffold and recipient’s vasculature which is crucial to the survival of tissues, and considerable tendency to develop constructs containing CD31+ endothelial cells which can contribute in vessel’s tube formation.

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