Using High-Throughput Screening to Evaluate Perturbations Potentially Linked to Neurobehavioral Outcomes: A Case Study Using Publicly Available Tools on FDA Batch-Certified Synthetic Food Dyes

2021 ◽  
Author(s):  
Nathalie Pham ◽  
Mark D. Miller ◽  
Melanie Marty
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Food Dyes ◽  
Neurobehavioral Outcomes

scholarly journals Action plan for hit identification (APHID): KAT6A as a case study

2020 ◽  
Vol 12 (5) ◽  
pp. 423-437 ◽  
Author(s):  
Xiangyan Yi ◽  
Lian Xue ◽  
Tim Thomas ◽  
Jonathan B Baell
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Action Plan ◽  
Hit Identification

Here, we describe our action plan for hit identification (APHID) that guides the process of hit triage, with elimination of less tractable hits and retention of more tractable hits. We exemplify the process with reference to our high-throughput screening (HTS) campaign against the enzyme, KAT6A, that resulted in successful identification of a tractable hit. We hope that APHID could serve as a useful, concise and digestible guide for those involved in HTS and hit triage, especially those that are relatively new to this exciting and continually evolving technology.

A systematic approach to prioritize drug targets using machine learning, a molecular descriptor-based classification model, and high-throughput screening of plant derived molecules: a case study in oral cancer

2015 ◽  
Vol 11 (12) ◽  
pp. 3362-3377 ◽  
Author(s):  
Vinay Randhawa ◽  
Anil Kumar Singh ◽  
Vishal Acharya
Keyword(s):  
Machine Learning ◽  
Oral Cancer ◽  
High Throughput ◽  
High Throughput Screening ◽  
Drug Targets ◽  
Systematic Approach ◽  
Molecular Descriptor ◽  
Classification Model

Network-based and cheminformatics approaches identify novel lead molecules forCXCR4, a key gene prioritized in oral cancer.

An integrated strategy for rapid screening of multi-target lead compounds for the treatment of Alzheimer's disease from traditional Chinese medicines by UHPLC combined with high-throughput screening: A case study on Salviae Miltiorrhizae Radix et Rhizoma

2021 ◽  
Vol 17 ◽  
Author(s):  
Minmin Zhang ◽  
Siduo Zhou ◽  
Wei Liu ◽  
Huijiao Yan ◽  
Xiao Wang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
High Throughput ◽  
Inhibitory Activity ◽  
High Throughput Screening ◽  
Hplc Analysis ◽  
Salviae Miltiorrhizae ◽  
Salviae Miltiorrhizae Radix ◽  
Ache Inhibitory Activity

Background: Salviae Miltiorrhizae Radix et Rhizoma (Red Sage root) is widely used in traditional Chinese medicine (TCM) for the treatment of Alzheimer’s disease (AD) with demonstrated curative effects, based on the concept of "one drug with multiple therapeutic targets," which appears to be a good strategy for AD treatment. Objective: This study aimed to develop of high-throughput screening (HTS) method for multi-therapeutic target components found in complex TCMs, which are active against AD, using Red Sage root as the case study. Method: Acetylcholinesterase (AChE) inhibitors (AChEIs) from Red Sage root extracts were pre-screened by ultrafiltration-HPLC (UF-HPLC) analysis, in which AChE was added to the extract and then ultrafiltered to remove non-binding compounds. Potential AChEIs were identified by HPLC analysis of compounds bound to AChE. A microplate-based HTS was then used to quantify the AChE inhibitory activity and antioxidant activity of the pre-screened compounds. Results: Pre-screening found ten potential inhibitors, which were identified by ESI-TOF/MS; six of these were purified by semi-preparative HPLC: Oleoyl neocryptotanshinone (1), Dihydrotanshinone Ⅰ (2), Cryptotanshinone (3), Tanshinone Ⅰ (4), Tanshinone ⅡA (5) and Miltirone (6). All six compounds had good AChE inhibitory activity and weak DPPH scavenging capacity. Conclusion: This study provides a platform and technology support for the rapid discovery of multi-target components, potentially active against AD, from complex TCMs and with strong potential for adaptation to the discovery of treatments for other diseases.

High-Throughput Screening of Thin-Film Semiconductor Material Libraries I: System Development and Case Study for TiWO

ChemSusChem ◽  
2015 ◽  
Vol 8 (7) ◽  
pp. 1270-1278 ◽  
Author(s):  
Kirill Sliozberg ◽  
Dominik Schäfer ◽  
Thomas Erichsen ◽  
Robert Meyer ◽  
Chinmay Khare ◽  
...  
Keyword(s):  
Thin Film ◽  
High Throughput ◽  
High Throughput Screening ◽  
System Development ◽  
Semiconductor Material

New high-throughput screening tool for the evaluation of pigmented UV-A curable coatings—A case study using low energy lamps

2006 ◽  
Vol 3 (4) ◽  
pp. 307-312 ◽  
Author(s):  
Stephanie Strazisar ◽  
Margaret Kendi ◽  
Thomas Fäcke ◽  
Leoné Hermans-Blackburn ◽  
Xudong Sharon Feng
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Tool ◽  
Low Energy

High Throughput Screening via Mass Spectrometry: A Case Study Using Acetylcholinesterase

2004 ◽  
Vol 2 (4) ◽  
pp. 373-381 ◽  
Author(s):  
Can C. Özbal ◽  
William A. LaMarr ◽  
John R. Linton ◽  
Donald F. Green ◽  
Arrin Katz ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
High Throughput Screening
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