scholarly journals Conditioning Film and Early Biofilm Succession on Plastic Surfaces

2021 ◽  
Author(s):  
Christoph D. Rummel ◽  
Oliver J. Lechtenfeld ◽  
René Kallies ◽  
Annegret Benke ◽  
Peter Herzsprung ◽  
...  
Keyword(s):  
Conditioning Film ◽  
Biofilm Succession ◽  
Plastic Surfaces

scholarly journals Capillary Effect Enhancement in a Plastic Capillary Tube by Nanostructured Surface

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 628
Author(s):  
Kazuma Kurihara ◽  
Ryohei Hokari ◽  
Naoki Takada
Keyword(s):  
Plasma Treatment ◽  
Capillary Tube ◽  
Capillary Effect ◽  
Nanostructured Surface ◽  
Nanostructure Fabrication ◽  
Wetting Properties ◽  
Treatment Processes ◽  
Standard Solution ◽  
The Cost ◽  
Plastic Surfaces

We investigated the enhancement of the capillary effect in a plastic capillary tube using only a nanostructured surface. Since plastic is a hydrophobic material, the capillary effect does not emerge without an additional coating or plasma treatment process. Therefore, capillary effect enhancement by the nanostructure fabrication method is expected to reduce the cost and minimise the contamination produced in the human body. By combining a hydrophilic nylon resin and a nanostructure at the tip of the plastic pipette, we could confirm that the capillary effect was produced solely by the tube fabrication process. The produced capillary effect increased linearly with increasing nanostructure height when a standard solution with a surface tension of 70 mN·m−1 was used. Thus, we can conclude that including the plastic part with nanostructure can be useful for biomedical applications. In addition, we suggest that the proposed method is highly effective in controlling the wetting properties of plastic surfaces, compared to the typical coating or plasma treatment processes.

scholarly journals Bond Strengthening in Oral Bacterial Adhesion to Salivary Conditioning Films

2008 ◽  
Vol 74 (17) ◽  
pp. 5511-5515 ◽  
Author(s):  
Henny C. van der Mei ◽  
Minie Rustema-Abbing ◽  
Joop de Vries ◽  
Henk J. Busscher
Keyword(s):  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Specific Interactions ◽  
Adhesion Forces ◽  
Bacterial Strains ◽  
Bacterial Interactions ◽  
Initial Adhesion ◽  
Conditioning Film ◽  
Interacting Surfaces ◽  
Conditioning Films

ABSTRACT Transition from reversible to irreversible bacterial adhesion is a highly relevant but poorly understood step in initial biofilm formation. We hypothesize that in oral biofilm formation, irreversible adhesion is caused by bond strengthening due to specific bacterial interactions with salivary conditioning films. Here, we compared the initial adhesion of six oral bacterial strains to salivary conditioning films with their adhesion to a bovine serum albumin (BSA) coating and related their adhesion to the strengthening of the binding forces measured with bacteria-coated atomic force microscopy cantilevers. All strains adhered in higher numbers to salivary conditioning films than to BSA coatings, and specific bacterial interactions with salivary conditioning films were accompanied by stronger initial adhesion forces. Bond strengthening occurred on a time scale of several tens of seconds and was slower for actinomyces than for streptococci. Nonspecific interactions between bacteria and BSA coatings strengthened twofold faster than their specific interactions with salivary conditioning films, likely because specific interactions require a closer approach of interacting surfaces with the removal of interfacial water and a more extensive rearrangement of surface structures. After bond strengthening, bacterial adhesion forces with a salivary conditioning film remained stronger than those with BSA coatings.

The fixation of anti-HBsAg on plastic surfaces

1982 ◽  
Vol 48 (2) ◽  
pp. 251-260 ◽  
Author(s):  
Janet Dobbins Place ◽  
Hartmut R. Schroeder
Keyword(s):  
Plastic Surfaces

The microbiome of oral cavity patients with periodontitis, adhesive and biofilm forming properties

2021 ◽  
Vol 66 (1) ◽  
pp. 45-51
Author(s):  
V. M. Chervinets ◽  
Yu. V. Chervinets ◽  
A. V. Leont’eva ◽  
E. A. Kozlova ◽  
N. M. Stulov ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Mucous Membrane ◽  
Healthy People ◽  
Periodontal Pocket ◽  
Adhesive Properties ◽  
Plastic Surface ◽  
Bacillus Spp ◽  
Adhesion Level ◽  
Severity Of The Disease ◽  
Plastic Surfaces

The microbiome of oral cavity in healthy people and patients with periodontitis was analyzed to determine their adhesive properties and the ability to form biofilms. The study involved 2 groups: healthy, 18 people, and an experimental group, 20 patients with chronic generalized periodontitis moderate severity of the disease. The average age of the studied people was 35-45 years. Material - dental plaque, scraping from the mucous membrane of the back of the tongue, the contents of the periodontal groove and periodontal pocket, as well as oral fluid. The main method of diagnostic was bacteriological. The average adhesion index (AAI) was used to determine adhesion level of microorganisms to epithelial cells of oral cavity’s mucous membrane. The microbiota’s ability to form biofilm was tested on glass and plastic surface. The microbiota of oral cavity of patients with periodontitis was characterized by decrease in the frequency of bacteria of the genera: Streptococcus, Peptostreptococcus, Peptococcus, and an increase in Staphylococcus aureus, Veillonella spp., Bacillus spp. The microbiota of the oral cavity of patients with generalized periodontitis has a greater ability to adhere to the cells of the mucous membrane than in healthy people, while their ability to form biofilms and exhibit pathogenic properties is enhanced. The biofilm formation of microorganisms in healthy and sick people differs both on glass and on plastic surfaces.

Efficacy of disinfectants against Cronobacter biofilm on plastic surfaces

2015 ◽  
Vol 1 (-1) ◽  
pp. 1-5
Author(s):  
E. Torlak ◽  
M. Gökmen ◽  
S. Aydemir
Keyword(s):  
Plastic Surfaces

scholarly journals Electrical microcurrent to prevent conditioning film and bacterial adhesion to urological stents

2010 ◽  
Vol 39 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Michael Gabi ◽  
Lukas Hefermehl ◽  
Danijela Lukic ◽  
Raphael Zahn ◽  
Janos Vörös ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Conditioning Film

Understanding urinary conditioning film components on ureteral stents: profiling protein components and evaluating their role in bacterial colonization

Biofouling ◽  
2013 ◽  
Vol 29 (9) ◽  
pp. 1115-1122 ◽  
Author(s):  
Chelsea N. Elwood ◽  
Joey Lo ◽  
Emily Chou ◽  
Adam Crowe ◽  
Olga Arsovska ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
Ureteral Stents ◽  
Conditioning Film ◽  
Protein Components

scholarly journals Pseudomonas aeruginosa-Mediated Damage Requires Distinct Receptors at the Apical and Basolateral Surfaces of the Polarized Epithelium

2009 ◽  
Vol 78 (3) ◽  
pp. 939-953 ◽  
Author(s):  
Iwona Bucior ◽  
Keith Mostov ◽  
Joanne N. Engel
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mdck Cells ◽  
Three Dimensional ◽  
Opportunistic Pathogen ◽  
Necessary And Sufficient ◽  
Plastic Surfaces ◽  
Pharmacologic Inhibition ◽  
Increased Susceptibility ◽  
Polarized Epithelium

ABSTRACT Pseudomonas aeruginosa, an important opportunistic pathogen of humans, exploits epithelial damage to establish infection. We have rigorously explored the role of N-glycoproteins and heparan sulfate proteoglycans (HSPGs) in P. aeruginosa-mediated attachment and subsequent downstream events at the apical (AP) and basolateral (BL) surfaces of polarized epithelium. We demonstrate that the N-glycan chains at the AP surface are necessary and sufficient for binding, invasion, and cytotoxicity to kidney (MDCK) and airway (Calu-3) cells grown at various states of polarization on Transwell filters. Upregulation of N-glycosylation enhanced binding, whereas pharmacologic inhibition of N-glycosylation or infection of MDCK cells defective in N-glycosylation resulted in decreased binding. In contrast, at the BL surface, the HS moiety of HSPGs mediated P. aeruginosa binding, cytotoxicity, and invasion. In incompletely polarized epithelium, HSPG abundance was increased at the AP surface, explaining its increased susceptibility to P. aeruginosa colonization and damage. Using MDCK cells grown as three-dimensional cysts as a model for epithelial organs, we show that P. aeruginosa specifically colocalized with HS-rich areas at the BL membrane but with complex N-glycans at the AP surface. Finally, P. aeruginosa bound to HS chains and N-glycans coated on plastic surfaces, showing the highest binding affinity toward isolated HS chains. Together, these findings demonstrate that P. aeruginosa recognizes distinct receptors on the AP and BL surfaces of polarized epithelium. Changes in the composition of N-glycan chains and/or in the distribution of HSPGs may explain the enhanced susceptibility of damaged epithelium to P. aeruginosa.

scholarly journals In vitro adherence of antibiotic-resistant Escherichia coli to biomaterial surfaces: Effect of conditioning film

2009 ◽  
Vol 11 (1) ◽  
Author(s):  
YB Ngwai ◽  
JA Onaolapo ◽  
JO Ehinmidu ◽  
K Ibrahim
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistant ◽  
Biomaterial Surfaces ◽  
Conditioning Film

scholarly journals Effects of Extracellular Matrix on the Morphology and Behaviour of Rabbit Auricular Chondrocytes in Culture

2005 ◽  
Vol 2005 (4) ◽  
pp. 364-373 ◽  
Author(s):  
Vega Villar-Suárez ◽  
B. Colaço ◽  
I. Calles-Venal ◽  
I. G. Bravo ◽  
J. G. Fernández-Álvarez ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Type Ii Collagen ◽  
Cell Phenotype ◽  
Nodule Formation ◽  
Type Ii ◽  
Protein Extract ◽  
Positive Effects ◽  
Reticular Pattern ◽  
Chondrocyte Cultures ◽  
Plastic Surfaces

Isolated chondrocytes dedifferentiate to a fibroblast-like shape on plastic substrata and proliferate extensively, but rarely form nodules. However, when dissociation is not complete and some cartilage remnants are included in the culture, proliferation decreases and cells grow in a reticular pattern with numerous nodules, which occasionally form small cartilage-like fragments. In an attempt to reproduce this stable chondrogenic state, we added a cartilage protein extract, a sugar extract, and hyaluronan to the medium of previously dedifferentiated chondrocytes. When protein extract was added, many cartilaginous nodules appeared. Hyaluronan produced changes in cell phenotype and behaviour, but not nodule formation. Protein extract has positive effects on the differentiation of previously proliferated chondrocytes and permits nodule formation and the extensive production of type-II collagen. A comparison with incompletely dissociated chondrocyte cultures suggests that the presence of some living cells anchored to their natural extracellular matrix provides some important additional factors for the phenotypical stability of chondrocytes on plastic surfaces. In order to elucidate if it is possible that the incidence of apoptosis is related to the results, we also characterized the molecular traits of apoptosis.

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