Characterization of Mechanisms of Glutathione Conjugation with Halobenzoquinones in Solution and HepG2 Cells

As a novel natural compound delivery system, liposomes are capable of incorporating lipophilic bioactive compounds with enhanced compound solubility, stability and bioavailability, and have been successfully translated into real-time clinical applications. To construct the soy phosphatidylcholine (SPC)–cholesterol (Chol) liposome system, the optimal formulation was investigated as 3:1 of SPC to Chol, 10% mannosylerythritol lipid-A (MEL-A) and 1% betulinic acid. Results show that liposomes with or without betulinic acid or MEL-A are able to inhibit the proliferation of HepG2 cells with a dose-effect relation remarkably. In addition, the modification of MEL-A in liposomes can significantly promote cell apoptosis and strengthen the destruction of mitochondrial membrane potential in HepG2 cells. Liposomes containing MEL-A and betulinic acid have exhibited excellent anticancer activity, which provide factual basis for the development of MEL-A in the anti-cancer applications. These results provide a design thought to develop delivery liposome systems carrying betulinic acid with enhanced functional and pharmaceutical attributes.

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Characterization of hepcidin response to holotransferrin in novel recombinant TfR1 HepG2 cells

Blood Cells Molecules and Diseases ◽

10.1016/j.bcmd.2016.06.008 ◽

2016 ◽

Vol 61 ◽

pp. 37-45 ◽

Cited By ~ 5

Author(s):

Kosha Mehta ◽

Mark Busbridge ◽

Derek Renshaw ◽

Robert W. Evans ◽

Sebastien Farnaud ◽

...

Keyword(s):

Hepg2 Cells

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The Rat Liver GlutathioneS-Transferase Ya Subunit Gene: Characterization of the Binding Properties of a Nuclear Protein from HepG2 Cells That Has High Affinity for the Antioxidant Response Element

Biochemistry ◽

10.1021/bi960572p ◽

1996 ◽

Vol 35 (35) ◽

pp. 11517-11521 ◽

Cited By ~ 34

Author(s):

Suxing Liu ◽

Cecil B. Pickett

Keyword(s):

Rat Liver ◽

Hepg2 Cells ◽

Nuclear Protein ◽

Antioxidant Response ◽

Antioxidant Response Element ◽

Subunit Gene ◽

Binding Properties ◽

Gene Characterization ◽

High Affinity

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Specific binding of free apolipoprotein A-I to a high-affinity binding site on HepG2 Cells: characterization of two high-density lipoprotein sites

Biochemistry ◽

10.1021/bi00174a047 ◽

1994 ◽

Vol 33 (8) ◽

pp. 2335-2340 ◽

Cited By ~ 25

Author(s):

Ronald Barbaras ◽

Xavier Collet ◽

Hugues Chap ◽

Bertrand Perret

Keyword(s):

High Density Lipoprotein ◽

Hepg2 Cells ◽

Specific Binding ◽

Density Lipoprotein ◽

Affinity Binding ◽

High Affinity Binding ◽

High Affinity ◽

Apolipoprotein A ◽

High Affinity Binding Site

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Study on structure characterization of pectin from the steamed ginseng and the inhibition activity of lipid accumulation in oleic acid-induced HepG2 cells

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2020.04.167 ◽

2020 ◽

Vol 159 ◽

pp. 57-65

Author(s):

Lili Jiao ◽

Hui Li ◽

Junming Li ◽

Li Bo ◽

Xiaoyu Zhang ◽

...

Keyword(s):

Oleic Acid ◽

Lipid Accumulation ◽

Hepg2 Cells ◽

Structure Characterization ◽

Inhibition Activity

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Characterization of glutathione conjugation in humans: Stereoselectivity in plasma elimination pharmacokinetics and urinary excretion of (R)- and (S)-2-bromoisovalerylurea in healthy volunteers

Clinical Pharmacology & Therapeutics ◽

10.1038/clpt.1993.8 ◽

1993 ◽

Vol 53 (1) ◽

pp. 49-58 ◽

Cited By ~ 6

Author(s):

Titia M T Mulders ◽

Vicki Venizelos ◽

Rik Schoemaker ◽

Adam F Cohen ◽

Douwe D Breimer ◽

...

Keyword(s):

Urinary Excretion ◽

Healthy Volunteers ◽

Glutathione Conjugation

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Chemical and Glutathione Conjugation-Related Degradation of Fotemustine: Formation and Characterization of a Glutathione Conjugate of Diethyl (1-Isocyanatoethyl)phosphonate, a Reactive Metabolite of Fotemustine

Chemical Research in Toxicology ◽

10.1021/tx00039a016 ◽

1994 ◽

Vol 7 (3) ◽

pp. 380-389 ◽

Cited By ~ 4

Author(s):

J. P. G. Brakenhoff ◽

J. N. M. Commandeur ◽

F. J. J. de Kanter ◽

B. L. M. van Baar ◽

W. C. M. M. Luijten ◽

...

Keyword(s):

Reactive Metabolite ◽

Glutathione Conjugate ◽

Glutathione Conjugation

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Characterization of Purified Red Cabbage Anthocyanins: Improvement in HPLC Separation and Protective Effect against H2O2-Induced Oxidative Stress in HepG2 Cells

Molecules ◽

10.3390/molecules24010124 ◽

2018 ◽

Vol 24 (1) ◽

pp. 124 ◽

Cited By ~ 6

Author(s):

Sheng Fang ◽

Fubin Lin ◽

Daofeng Qu ◽

Xianrui Liang ◽

Liping Wang

Keyword(s):

Oxidative Stress ◽

Hepg2 Cells ◽

Antioxidant Activities ◽

Hplc Separation ◽

Column Temperature ◽

Red Cabbage ◽

Chemical Profiles ◽

Derivatives Of ◽

Intracellular Oxidative Stress

In this study, the chemical profiles and antioxidant activities of red cabbage anthocyanin (RCA)-enriched extract are evaluated. The effects of column temperature on the HPLC resolution of the RCAs are studied. The HPLC resolutions became better as the column temperature increased from 20 °C–45 °C. An optimized HPLC condition was achieved at 45 °C and used for the quantification and qualification of the RCAs. The anthocyanins in the enriched powder are all derivatives of cyanidin (268 ± 2 μg/mg), mainly with 19% nonacylated, 51% monoacylated, and 31% diacylated structures with ferulic, sinapic, p-coumaric, and caffeic acids characterized by HPLC-MS. The RCA extracts markedly reduced intracellular oxidative stress production by H2O2 on HepG2 cells and consequently ameliorated cell apoptosis and improved viability. The analytical method and cellular antioxidant activity demonstration of the RCAs will greatly facilitate their functional applications.

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