SERS-Based Lateral Flow Strip Biosensor for Simultaneous Detection of Listeria monocytogenes and Salmonella enterica Serotype Enteritidis

2017 ◽  
Vol 65 (47) ◽  
pp. 10290-10299 ◽  
Author(s):  
Hai-bin Liu ◽  
Xin-jun Du ◽  
Yu-Xuan Zang ◽  
Ping Li ◽  
Shuo Wang
Keyword(s):  
Listeria Monocytogenes ◽  
Salmonella Enterica ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Salmonella Enterica Serotype Enteritidis

scholarly journals A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens

2021 ◽  
Vol 18 (9) ◽  
pp. 4574
Author(s):  
Kai Chen ◽  
Biao Ma ◽  
Jiali Li ◽  
Erjing Chen ◽  
Ying Xu ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Quantitative Detection ◽  
Bacterial Strains ◽  
Food Borne Pathogens ◽  
Food Borne

Food-borne pathogens have become an important public threat to human health. There are many kinds of pathogenic bacteria in food consumed daily. A rapid and sensitive testing method for multiple food-borne pathogens is essential. Europium nanoparticles (EuNPs) are used as fluorescent probes in lateral flow immunoassays (LFIAs) to improve sensitivity. Here, recombinase polymerase amplification (RPA) combined with fluorescent LFIA was established for the simultaneous and quantitative detection of Listeria monocytogenes, Vibrio parahaemolyticus, and Escherichia coliO157:H7. In this work, the entire experimental process could be completed in 20 min at 37 °C. The limits of detection (LODs) of EuNP-based LFIA–RPA were 9.0 colony-forming units (CFU)/mL for Listeria monocytogenes, 7.0 CFU/mL for Vibrio parahaemolyticus, and 4.0 CFU/mL for Escherichia coliO157:H7. No cross-reaction could be observed in 22 bacterial strains. The fluorescent LFIA–RPA assay exhibits high sensitivity and good specificity. Moreover, the average recovery of the three food-borne pathogens spiked in food samples was 90.9–114.2%. The experiments indicate the accuracy and reliability of the multiple fluorescent test strips. Our developed EuNP-based LFIA–RPA assay is a promising analytical tool for the rapid and simultaneous detection of multiple low concentrations of food-borne pathogens.

scholarly journals Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

RSC Advances ◽  
2018 ◽  
Vol 8 (17) ◽  
pp. 9580-9586 ◽  
Author(s):  
Yaning Sun ◽  
Jifei Yang ◽  
Suzhen Yang ◽  
Qingbo Sang ◽  
Man Teng ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Quantitative Detection ◽  
Lateral Flow Strip ◽  
Immunochromatographic Strip ◽  
Gentamicin Sulfate ◽  
Kanamycin Sulfate ◽  
Neomycin Sulfate

A colloidal gold-based immunochromatographic strip has been developed for the rapid, simultaneous, semi-quantitative and quantitative detection of several aminoglycoside residues in milk, including gentamicin sulfate (GM), neomycin sulfate (NEO) and kanamycin sulfate (KN).

scholarly journals Simultaneous Detection of Multiple β-Adrenergic Agonists with 2-Directional Lateral Flow Strip Platform

2020 ◽  
Vol 36 (6) ◽  
pp. 653-658
Author(s):  
Qian WU ◽  
Qing SONG ◽  
Xinxin WANG ◽  
Li YAO ◽  
Jianguo XU ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Lateral Flow ◽  
Adrenergic Agonists ◽  
Lateral Flow Strip

Ultrasensitive detection of seventeen chemicals simultaneously using paper-based sensors

2018 ◽  
Vol 2 (10) ◽  
pp. 1900-1910 ◽  
Author(s):  
Zhongxing Wang ◽  
Li Sun ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Liguang Xu ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Ultrasensitive Detection ◽  
Lateral Flow Strip

We developed an ultrasensitive gold nanoparticle-based multicomponent lateral-flow strip assay for the simultaneous detection of seventeen hormone drugs from three classes: nandrolone (NR) and its analogues, dexamethasone (DEX) and its analogues, and hexestrol (HES) and its analogues.

scholarly journals Simultaneous detection of food safety hazards (Salmonella enterica and Listeria monocytogenes) in table eggs using multiplex PCR

2020 ◽  
Vol 1 (2) ◽  
pp. 98-103
Author(s):  
C. O. Vinayananda ◽  
Mohamed Nadeem Fairoze ◽  
C. B. Madhavaprasad ◽  
Nagappa S. K.
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Food Safety ◽  
Foodborne Pathogens ◽  
Salmonella Enterica ◽  
Simultaneous Detection ◽  
Safety Hazards ◽  
Safety Standards ◽  
Cultural Methods ◽  
Health Significance

Abstract: Foods are analyzed for food safety hazards in order to comply with food safety standards. Among food-borne pathogens Salmonella enterica and Listeria monocytogenes are recognized as major foodborne pathogens of public health significance worldwide. In the present study, multiplex polymerase chain reaction (mPCR) was used to screen for S. enterica and L. monocytogenes in table eggs collected from different markets (840 eggs). Pooled egg samples categorized as commercial and backyard eggs based on the source of collection. Collected eggs were screened for S. enterica and L. monocytogenes using mPCR assays. Conserved regions viz. invA and prfA genes were targeted for the specific detection of S. enterica and L. monocytogenes, respectively. mPCR and conventional method showed same results, prevalence of S. enterica at 12.5 and 33.3 per cent in commercial and backyard eggs, respectively. While, L. monocytogenes was undetectable in commercial eggs; but, detected only in backyard table egg sample (8.3%). Present study indicated complete concordance between specific pre-enrichment mPCR and conventional cultural methods. Results of the study underscored mPCR as steadfast rapid tool for the screening of table eggs for listed food safety hazards S. enterica and L. monocytogenes in table eggs. Keywords: Eggs; Listeria; mPCR; Public Health; Salmonella

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