In Vitro and in Vivo Anti-Hyperglycemic Activities of Taxifolin and Its Derivatives Isolated from Pigmented Rice (Oryzae sativa L. cv. Superhongmi)

2019 ◽  
Vol 68 (3) ◽  
pp. 742-750
Author(s):  
Kee Dong Yoon ◽  
Jung-Yun Lee ◽  
Tae Yang Kim ◽  
Hanna Kang ◽  
Kyoung-Soo Ha ◽  
...  
Keyword(s):  
Pigmented Rice ◽  
Oryzae Sativa

scholarly journals STUDI LITERATUR PEMANFAATAN SINTESIS SENYAWA PIPERIDINIL-P-KUMARAMIDA DARI ASAM-P-KUMARAT YANG DIPEROLEH DARI LIMBAH SEKAM PADI (ORYZAE SATIVA) SEBAGAI OBAT ANTI KANKER LEUKEMIA

2021 ◽  
Vol 8 (2) ◽  
pp. 28-36
Author(s):  
Athalia Theda Tanujaya ◽  
Steven Steven ◽  
Norman Emil Ramadhan
Keyword(s):  
Oryzae Sativa

Pendahuluan: Pada tahun 2018, angka kejadian kanker di Indonesia mencapai 136,2 per 100.000 penduduk serta kanker leukemia menduduki posisi kelima sebagai kanker paling mematikan dan telah merenggut 11.314 jiwa. Tingginya biaya pengobatan leukemia menjadikan penyerapan anggaran pembiayaan dalam program JKN-KIS sebesar Rp3,4 triliun. Melihat masalah tersebut, para peneliti mencoba memanfaatkan bahan alam untuk mengembangkan agen anti-leukemia yang baru. Isi: Salah satu senyawa yang dapat menghambat kanker leukemia adalah piperidinil-p-kumaramida, turunan dari asam-p-kumarat yang dapat ditemukan dan diisolasi dari limbah tanaman padi atau Oryzae sativa. Setiap tahunnya, 79,2 juta ton tanaman padi diproduksi dan menghasilkan 19,8 juta ton limbah sekam. Tujuan dari artikel ini adalah untuk mengoptimalisasi pemanfaatan limbah sekam padi sebagai prekursor piperidinil-p-kumaramida untuk antikanker leukemia. Metode: Metode yang digunakan adalah ekstraksi bertingkat limbah sekam dengan menggunakan larutan air-metanol dan etil asetat. Ekstrak lalu diisolasi dan dipurifikasi KLT. Setelah itu, penetapan kadar dianalisis menggunakan KCKT. Asam-p-kumarat dimodifikasi menjadi piperidinil-p-kumaramida dengan cara mereaksikannya dengan piperidin. Kesimpulan: Aktivitas piperidinil-p-kumaramida sebagai anti kanker leukemia pada sel p388 dapat diuji secara in vitro dan in vivo. Berdasarkan studi literatur yang telah dilakukan, sebanyak 100 gram limbah sekam padi dapat menghasilkan 265.4 ± 2.4 mg asam p-kumarat. Setiap 0,5 gram asam-p-kumarat dapat yang dapat disintesis menjadi piperidinil-p-kumaramida yang mempunyai IC50 sebesar 5,34 µg/mL sehingga dapat dimanfaatkan sebagai agen anti kanker leukemia.  

scholarly journals Impact of Oral Mastication on the in vitro Digestibility of Pigmented and Non-Pigmented Rice Varieties

2021 ◽  
Vol 12 (1) ◽  
pp. 1148-1160
Keyword(s):  
Amylose Content ◽  
Starch Content ◽  
In Vitro Digestion ◽  
In Vitro Digestibility ◽  
Rice Varieties ◽  
Pigmented Rice ◽  
Rice Samples ◽  
Oral Processing

The extent of starch hydrolysis and glycemic index (GI) of foods depends on how it is orally processed, but many in vitro digestion studies failed to consider the oral phase of digestion. This research aimed to understand the relationship between oral mastication and GI of rice. For this study, different rice varieties (pigmented and non-pigmented) were selected and analyzed for their physicochemical properties. The amylose content for all rice varieties was higher than 25%, with the starch content of 68.68 ± 0.70% - 81.60 ± 1.78%. Temporal dominance of sensation was determined for rice samples to understand the consumers' sensory preferences towards the pigmented rice varieties. In vivo oral mastication studies were also performed for the rice varieties, in which significant differences were observed amongst pigmented and non-pigmented rice samples. The particle size for pigmented rice varieties after in vivo oral mastication was significantly larger (50% particles greater than 2 mm; due to its intact morphology) than non-pigmented rice. The significant impact of oral processing on the GI of rice irrespective of the varieties was also observed in this study. Thus, this research sheds light on the need for oral processing for in vitro digestion studies.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

Ultrastructure of melanocyte-keratinocyte interactions and pigment transfer in vitro

1978 ◽  
Vol 36 (2) ◽  
pp. 650-651
Author(s):  
Raul I. Garcia ◽  
Evelyn A. Flynn ◽  
George Szabo
Keyword(s):  
Direct Injection ◽  
Skin Pigmentation ◽  
Freeze Fracture ◽  
Pigment Granule ◽  
Time Lapse ◽  
Ultrastructural Level ◽  
Lanthanum Tracer ◽  
A Cell

Skin pigmentation in mammals involves the interaction of epidermal melanocytes and keratinocytes in the structural and functional unit known as the Epidermal Melanin Unit. Melanocytes(M) synthesize melanin within specialized membrane-bound organelles, the melanosome or pigment granule. These are subsequently transferred by way of M dendrites to keratinocytes(K) by a mechanism still to be clearly defined. Three different, though not necessarily mutually exclusive, mechanisms of melanosome transfer have been proposed: cytophagocytosis by K of M dendrite tips containing melanosomes, direct injection of melanosomes into the K cytoplasm through a cell-to-cell pore or communicating channel formed by localized fusion of M and K cell membranes, release of melanosomes into the extracellular space(ECS) by exocytosis followed by K uptake using conventional phagocytosis. Variability in methods of transfer has been noted both in vivo and in vitro and there is evidence in support of each transfer mechanism. We Have previously studied M-K interactions in vitro using time-lapse cinemicrography and in vivo at the ultrastructural level using lanthanum tracer and freeze-fracture.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

Probing the ultrastructure of the mitotic and meiotic spindle in eggs: An expeditious approach based on semi-thin (0.25 μm) serial sections

1983 ◽  
Vol 41 ◽  
pp. 552-555
Author(s):  
Conly L. Rieder ◽  
S. Bowser ◽  
R. Nowogrodzki ◽  
K. Ross ◽  
G. Sluder
Keyword(s):  
Small Sample Size ◽  
Small Sample ◽  
Meiotic Spindle ◽  
Serial Sections ◽  
Mitotic Apparatus ◽  
Thin Sections ◽  
Cell Cycles ◽  
Ultrastructural Studies

Eggs have long been a favorite material for studying the mechanism of karyokinesis in-vivo and in-vitro. They can be obtained in great numbers and, when fertilized, divide synchronously over many cell cycles. However, they are not considered to be a practical system for ultrastructural studies on the mitotic apparatus (MA) for several reasons, the most obvious of which is that sectioning them is a formidable task: over 1000 ultra-thin sections need to be cut from a single 80-100 μm diameter egg and of these sections only a small percentage will contain the area or structure of interest. Thus it is difficult and time consuming to obtain reliable ultrastructural data concerning the MA of eggs; and when it is obtained it is necessarily based on a small sample size.We have recently developed a procedure which will facilitate many studies concerned with the ultrastructure of the MA in eggs. It is based on the availability of biological HVEM's and on the observation that 0.25 μm thick serial sections can be screened at high resolution for content (after mounting on slot grids and staining with uranyl and lead) by phase contrast light microscopy (LM; Figs 1-2).

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