Complementary iTRAQ Proteomic and Transcriptomic Analyses of Leaves in Tea Plant (Camellia sinensisL.) with Different Maturity and Regulatory Network of Flavonoid Biosynthesis

Abstract Background: Abscisic acid (ABA) is an important phytohormone responsible for activating drought resistance, but the regulation mechanism of exogenous ABA on tea plants under drought stress was rarely reported. Results: The results showed that the exogenous ABA significantly induced the metabolic pathways of tea leaves under drought stress, including the chlorophyll synthesis, photosynthesis, sucrose and starch metabolism, TCA cycle, glycolysis, lipid metabolism and flavonoids biosynthesis. In which, the exogenous ABA could up-regulated the genes related to lipid metabolism and flavonoid biosynthesis, including LPCAT , ALDH, FLS, CHI, DFR, and down-regulated the genes related to lipid metabolism and flavonoid biosynthesis, including FATB, EKI, DGK , PAL, 4CL . The exogenous ABA could also increase the contents of flavone, anthocyanins, flavonol, isoflavone of tea leaves under drought stress, including delphinidin 3-O-glucosidewere, cyanidin 3-O-rutinoside, kaempferitrin, sakuranetin, prunetin, kaempferol, and decrease the contents of glycerophospholipids, glycerolipids and fatty acids of tea leaves under drought stress, including LysoPE 14:0, LysoPE 16:0, LysoPE 18:0, LysoPE 18:1, LysoPC 15:1 and LysoPC 16:0. And there were strong correlations between the genes and metabolites. Conclusions: The results suggested that the exogenous ABA could alleviate the damages of tea leaves under drought stress through inducing the expressions of the genes and altering the contents of metabolites in response to drought stress. The data also provide a good foundation for further research on the roles of the genes and metabolites in response to ABA.

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In-Silico Identification, Expressional Profile and Regulatory Network Analysis of Mitogen Activated Protein Kinase Kinase Kinase (MAPKKK) Gene Family in Tea (C. Sinensis)

10.21203/rs.3.rs-56865/v1 ◽

2020 ◽

Author(s):

Abhirup Paul ◽

Anurag P. Srivas ◽

Shreya Subrahmanya ◽

Guoxin Shen ◽

NEELAM MISHRA

Keyword(s):

Protein Kinase ◽

Regulatory Network ◽

Tea Plant ◽

Stress Factors ◽

Mitogen Activated Protein Kinase ◽

Functional Study ◽

Functional Domain ◽

A Genome ◽

Mitogen Activated Protein ◽

Protein Kinase Kinase

Abstract Background: Mitogen activated protein kinase kinase kinase (MAPKKK) form the upstream component of MAPK cascade. It is well characterized in several plants such as Arabidopsis and rice however the knowledge about MAPKKKs in tea plant is largely unknown.Result: In the present study, MAPKKK genes of tea were obtained through a genome wide search using Arabidopsis thaliana as the reference genome. Among 59 candidate MAPKKK genes in tea, 17 genes were MEKK-like, 31 genes were Raf-like and 11 genes were ZIK- like. Additionally, phylogenetic relationships were established along with structural analysis which includes gene structure, its location as well as conserved motifs and functional domain signatures that were systematically examined and further, predictions were validated by the results. Also, on the basis of orthologous genes in Arabidopsis, functional interaction was carried out in C. sinensis. The expressional profiles indicated major involvement of MAPKKKK genes from tea in response to various abiotic stress factors.Conclusion: The present study provides the targets for additional inclusive identification, functional study, and also might provide comprehensive knowledge for a better understanding of the MAPKKK cascade regulatory network in C. sinensis.

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