Near-Field Plasmonic Probe with Super Resolution and High Throughput and Signal-to-Noise Ratio

Nano Letters ◽  
2018 ◽  
Vol 18 (2) ◽  
pp. 881-885 ◽  
Author(s):  
Ruei-Han Jiang ◽  
Chi Chen ◽  
Ding-Zheng Lin ◽  
He-Chun Chou ◽  
Jen-You Chu ◽  
...  
Keyword(s):  
High Throughput ◽  
Signal To Noise Ratio ◽  
Near Field ◽  
Super Resolution ◽  
Signal To Noise ◽  
Noise Ratio

scholarly journals Near-field sound source localization using principal component analysis–multi-output support vector regression

2020 ◽  
Vol 16 (4) ◽  
pp. 155014772091640
Author(s):  
Lanmei Wang ◽  
Yao Wang ◽  
Guibao Wang ◽  
Jianke Jia
Keyword(s):  
Principal Component Analysis ◽  
Support Vector Regression ◽  
Covariance Matrix ◽  
Signal To Noise Ratio ◽  
Near Field ◽  
Principal Component ◽  
Component Analysis ◽  
Support Vector ◽  
Signal To Noise ◽  
Noise Ratio

In this article, principal component analysis method, which is applied to image compression and feature extraction, is introduced into the dimension reduction of input characteristic variable of support vector regression, and a method of joint estimation of near-field angle and range based on principal component analysis dimension reduction is proposed. Signal-to-noise ratio and calculation amount are the decisive factors affecting the performance of the algorithm. Principal component analysis is used to fuse the main characteristics of training data and discard redundant information, the signal-to-noise ratio is improved, and the calculation amount is reduced accordingly. Similarly, support vector regression is used to model the signal, and the upper triangular elements of the signal covariance matrix are usually used as input features. Since the covariance matrix has more upper triangular elements, training it as a feature input will affect the training speed to some extent. Principal component analysis is used to reduce the dimensionality of the upper triangular element of the covariance matrix of the known signal, and it is used as the input feature of the multi-output support vector regression machine to construct the near-field parameter estimation model, and the parameter estimation of unknown signal is herein obtained. Simulation results show that this method has high estimation accuracy and training speed, and has strong adaptability at low signal-to-noise ratio, and the performance is better than that of the back-propagation neural network algorithm and the two-step multiple signal classification algorithm.

scholarly journals Evaluation of sted super-resolution image quality by image correlation spectroscopy (QuICS)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elena Cerutti ◽  
Morgana D’Amico ◽  
Isotta Cainero ◽  
Gaetano Ivan Dellino ◽  
Mario Faretta ◽  
...  
Keyword(s):  
Autocorrelation Function ◽  
Signal To Noise Ratio ◽  
Super Resolution ◽  
Image Contrast ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Signal To Noise ◽  
Image Correlation Spectroscopy ◽  
Noise Ratio

AbstractQuantifying the imaging performances in an unbiased way is of outmost importance in super-resolution microscopy. Here, we describe an algorithm based on image correlation spectroscopy (ICS) that can be used to assess the quality of super-resolution images. The algorithm is based on the calculation of an autocorrelation function and provides three different parameters: the width of the autocorrelation function, related to the spatial resolution; the brightness, related to the image contrast; the relative noise variance, related to the signal-to-noise ratio of the image. We use this algorithm to evaluate the quality of stimulated emission depletion (STED) images of DNA replication foci in U937 cells acquired under different imaging conditions. Increasing the STED depletion power improves the resolution but may reduce the image contrast. Increasing the number of line averages improves the signal-to-noise ratio but facilitates the onset of photobleaching and subsequent reduction of the image contrast. Finally, we evaluate the performances of two different separation of photons by lifetime tuning (SPLIT) approaches: the method of tunable STED depletion power and the commercially available Leica Tau-STED. We find that SPLIT provides an efficient way to improve the resolution and contrast in STED microscopy.

scholarly journals Super-Resolution Reconstruction of Cell Pseudo-Color Image Based on Raman Technology

Sensors ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 4076
Author(s):  
Yang ◽  
Zhu ◽  
Wang ◽  
Yang ◽  
Wu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Raman Spectrum ◽  
Background Noise ◽  
Color Image ◽  
Signal To Noise Ratio ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
Signal To Noise ◽  
Noise Ratio ◽  
Pseudo Color

Raman spectroscopy visualization is a challenging task due to the interference of complex background noise and the number of selected measurement points. In this paper, a super-resolution image reconstruction algorithm for Raman spectroscopy is studied to convert raw Raman data into pseudo-color super-resolution imaging. Firstly, the Raman spectrum data of a single measurement point is measured multiple times to calculate the mean value to remove the random background noise, and innovatively introduce the Retinex algorithm and the median filtering algorithm which improve the signal-to-noise ratio. The novel method of using deep neural network performs a super-resolution reconstruction operation on the gray image. An adaptive guided filter that automatically adjusts the filter radius and penalty factor is proposed to highlight the contour of the cell, and the super-resolution reconstruction of the pseudo-color image of the Raman spectrum is realized. The average signal-to-noise ratio of the reconstructed pseudo-color image sub-band reaches 14.29 db, and the average value of information entropy reaches 4.30 db. The results show that the Raman-based cell pseudo-color image super-resolution reconstruction algorithm is an effective tool to effectively remove noise and high-resolution visualization. The contrast experiments show that the pseudo-color image Kullback–Leiber (KL) entropy of the color image obtained by the method is small, the boundary is obvious, and the noise is small, which provide technical support for the development of sophisticated single-cell imaging Raman spectroscopy instruments.

Fast Super-Resolution Fluorescence Microscopy Imaging with Low Signal-to-Noise Ratio Based on Deep Learning

2020 ◽  
Vol 47 (10) ◽  
pp. 1007002
Author(s):  
肖康 Xiao Kang ◽  
田立君 Tian Lijun ◽  
王中阳 Wang Zhongyang
Keyword(s):  
Deep Learning ◽  
Fluorescence Microscopy ◽  
Signal To Noise Ratio ◽  
Super Resolution ◽  
Signal To Noise ◽  
Microscopy Imaging ◽  
Noise Ratio
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