Fabrication of a Silk Sericin Hydrogel System Delivering Human Lactoferrin Using Genetically Engineered Silk with Improved Bioavailability to Alleviate Chemotherapy-Induced Immunosuppression

2021 ◽  
Author(s):  
Sheng Xu ◽  
Huanhuan Tan ◽  
Qianqian Yang ◽  
Riyuan Wang ◽  
Chi Tian ◽  
...  
Keyword(s):  
Genetically Engineered ◽  
Human Lactoferrin ◽  
Silk Sericin ◽  
Hydrogel System

SELECTION WORK WITH STOCK OF TRANSGENIC GOATS IN THE REPUBLIC OF BELARUS

2021 ◽  
pp. 16-19
Author(s):  
A.N. RUDAK ◽  
Keyword(s):  
Genetically Engineered ◽  
Comparative Assessment ◽  
Human Lactoferrin ◽  
Transgenic Goats ◽  
The Republic ◽  
Selection Work

Genealogical structure of the livestock of goats producing the bio-analogue of human lactoferrin has been studied for the fi rst time in the Republic of Belarus. Successors of lines and families have been determined, and a comparative assessment of using various selection profi les for obtaining goats and dams of the stock of genetically engineered animals has been carried out.

226 HUMAN LACTOFERRIN EXPRESSED IN COW MILK CAN EXTEND THE SHELF LIFE OF MILK

2014 ◽  
Vol 26 (1) ◽  
pp. 226
Author(s):  
S. N. Lotti ◽  
A. Cooper ◽  
J. D. Murray
Keyword(s):  
Shelf Life ◽  
Repeated Measures ◽  
Room Temperature ◽  
Antimicrobial Agents ◽  
Genetically Engineered ◽  
Cow Milk ◽  
Human Lactoferrin ◽  
Antimicrobial Protein ◽  
Control Milk ◽  
Growth Of Bacteria

Lactoferrin (LF) is an antimicrobial protein that is produced at high levels in human secretions, including milk, but is present at very low levels in the milk of ruminants. Lactoferrin is an iron-binding protein, and because iron is required by some bacteria to grow, lactoferrin can reduce the growth of certain bacteria. Lactoferrin can inhibit the growth of gram-negative bacteria, gram-positive bacteria, yeast, and some antibiotic-resistant pathogens. Lactoferrin also has bacteriocidal activity, killing the organism by inducing membrane perturbations often in conjunction with other antimicrobial agents such as lysozyme. Due to these properties, lactoferrin has the potential to increase the health of lactating animals as well as extend the shelf life of the milk. A herd of cows were genetically engineered to produce milk containing recombinant human lactoferrin (rhLF-milk) at approximately the same concentration found in human milk. In this study, growth of bacteria in raw milk samples from 2 control cows was compared with that from 2 cows that produce rhLF-milk. The experiment examined the shelf life of milk at room temperature over a 72-h period and the shelf life of milk left at 4°C over 3 weeks by plating aliquots on agar plates and counting the number of bacterial colonies that grew on plates after 24 and 48 h. The milk left at room temperature was plated at 0, 24, 48, and 72 h. The milk left in at 4°C was measured at 0, 4, 7, 11, 14, 18, and 21 days. Statistical evaluations were conducted using SAS statistical software, version 9.3 (SAS Institute Inc., Cary, NC, USA). Bacterial data were compared using the repeated-measures function in SAS and the Greenhouse-Geisser epsilon test to determine differences. For all tests, P-values <0.05 were considered statistically significant. The rhLF-milk showed less bacteria growth than control milk over the 4 time periods when left at room temperature (P = 0.0029), but did not inhibit the growth of bacteria when held for longer periods at 4°C (P = 0.6031). This shows that rhLF-milk may have an extended shelf life at room temperature and thus may be a means to increase food availability in developing countries. The next step in this research will be examining the populations of bacteria in the milk to determine how rhLF alters the populations of bacteria growing in milk over time, the different types of bacteria populations between the rhLF and control milk and what their effects are.

Reproductive health issues in patients with psoriasis (literature review)

2019 ◽  
Vol 1 (7) ◽  
pp. 5-8
Author(s):  
L. S. Kruglova ◽  
A. A. Osina ◽  
A. A. Khotko
Keyword(s):  
Drug Therapy ◽  
Reproductive Health ◽  
Literature Review ◽  
Pregnancy Outcomes ◽  
Adverse Pregnancy Outcome ◽  
Certolizumab Pegol ◽  
Genetically Engineered ◽  
Health Issues ◽  
Degree Of Confidence ◽  
Adverse Pregnancy

Among patients with psoriasis, approximately 50% are women and almost 75 % of them are under the age of 40 years. Thus, most women with psoriasis have childbearing potential. When pregnancy occurs in 22 % of patients, the activity of psoriasis persists, characteristic of the course before pregnancy, in 23 % of women, the course of the disease worsens. The article provides up-to-date data on the management of pregnant patients with psoriasis. To improve pregnancy outcomes in patients with psoriasis, it is important to prevent exacerbation of the disease. The choice of drug therapy in this case is based on an assessment of the ratio of the risk of undesirable effects of the drugs on the developing fetus and the risk of the development of exacerbation of psoriasis, which can cause an adverse pregnancy outcome. Despite the fact that the available clinical experience of using genetically engineered drugs is still limited, with a certain degree of confidence we can say that there is no increase in the risk of adverse pregnancy outcomes associated with therapy with certolizumab pegol.

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

SELECTION AND SWITCHING OF GENETICALLY ENGINEERED BIOLOGICAL AGENTS IN TREATMENT OF JUVENILE ARTHRITIS

2018 ◽  
Vol 97 (3) ◽  
pp. 52-61
Author(s):  
E.S. Zholobova ◽  
◽  
A.K. Ignatova ◽  
N.G. Seylanova ◽  
A.P. Golubeva ◽  
...  
Keyword(s):  
Biological Agents ◽  
Genetically Engineered ◽  
Juvenile Arthritis

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

2018 ◽  
Author(s):  
Suying Xu ◽  
Adam Sedgwick ◽  
Souad Elfecky ◽  
Wenbo Chen ◽  
Ashley Jones ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Boronic Acid ◽  
Binding Constants ◽  
Strong Fluorescence ◽  
Fluorescence Response ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Hydrogel System

<p>A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection<i>. </i>In solution, the fluorescent probe<b> </b>displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M). <b><u></u></b></p>

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