Characterization of Calcium Phosphate Nanoparticles Based on a PEGylated Chelator for Gene Delivery

2017 ◽  
Vol 9 (12) ◽  
pp. 10435-10445 ◽  
Author(s):  
Xiangang Huang ◽  
Diana Andina ◽  
Jingshui Ge ◽  
Anne Labarre ◽  
Jean-Christophe Leroux ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Gene Delivery ◽  
Calcium Phosphate Nanoparticles

Preparation and Characterization of Calcium Phosphate Nanoparticles

2003 ◽  
Vol 254-256 ◽  
pp. 903-906 ◽  
Author(s):  
C.M. Manuel ◽  
M. Foster ◽  
F.J. Monteiro ◽  
M.P. Ferraz ◽  
Robert H. Doremus ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Nanoparticles

PREPARATION OF CALCIUM PHOSPHATE NANOPARTICLES AS NON-VIRAL VECTORS FOR GENE DELIVERY SYSTEM

2017 ◽  
Vol 29 (04) ◽  
pp. 1750027 ◽  
Author(s):  
Ko-Chung Yen ◽  
I-Hua Chen ◽  
Feng-Huei Lin
Keyword(s):  
Gene Therapy ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Delivery System ◽  
Viral Vectors ◽  
Fluorescent Protein ◽  
Molar Ratio ◽  
Gene Delivery System ◽  
Calcium Phosphate Nanoparticles

A major aim of gene therapy is the efficient and specific delivery of therapeutic gene into the desired target tissues. Development of reliable vectors is a major challenge in gene therapy. The aim of this study is to develop calcium phosphate nanoparticles as novel non-viral vectors for the gene delivery system. Calcium phosphate nanoparticles were prepared by water-in-oil microemulsion method with a water to surfactant molar ratio, Wo [Formula: see text] 2–10. This paper studies the design and synthesis of ultra-low size, highly monodispersed DNA doped calcium phosphate nanoparticles of size around 100[Formula: see text]nm in diameter. The structure of DNA-calcium phosphate nanocomplex observed by TEM was displayed as a shell-like structure. This study used pEGFP as a reporter gene. The encapsulating efficiency to encapsulate DNA inside the nanoparticles was greater than 80%. In the MTT test, both calcium phosphate nanoparticles and DNA-calcium phosphate nanocomplex have no negative effect for 293T cells. By gel electrophoresis of free and entrapped pEGFP DNA, the DNA encapsulated inside the nanoparticles was protected from the external DNaseI environment. In vitro transfection studies in 293T cell-line, the DNA-calcium phosphate nanocomplex could be used safely to transfer the encapsulated DNA into the 293T cells and expression green fluorescent protein. The characteristic of DNA-calcium phosphate nanocomplex to deliver DNA belongs to slow release. The property of DNA-calcium phosphate nanocomplex was fit in the requirement of non-viral vectors for the gene delivery system.

scholarly journals SYNTHESIS AND CHARACTERIZATION OF CALCIUM PHOSPHATE NANOPARTICLES AND EFFECT OF THE AGITATION TYPE ON PARTICLES MORPHOLOGY

2019 ◽  
Vol 19 (1) ◽  
pp. 284-298
Author(s):  
E.A. Flores-Hernández ◽  
◽  
R.H. Lira-Saldívar ◽  
R. Acosta-Ortiz ◽  
B. Méndez-Arguello ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Synthesis And Characterization ◽  
Calcium Phosphate Nanoparticles

Calcium phosphate nanoparticles prepared from infusion fluids for stem cell transfection: process optimization and cytotoxicity analysis

2017 ◽  
Vol 5 (5) ◽  
pp. 972-981 ◽  
Author(s):  
Quazi T. H. Shubhra ◽  
Ayako Oyane ◽  
Hiroko Araki ◽  
Maki Nakamura ◽  
Hideo Tsurushima
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Process Optimization ◽  
Cell Transfection ◽  
Calcium Phosphate Nanoparticles ◽  
Infusion Fluids

The preparation of calcium phosphate nanoparticles from infusion fluids for gene delivery to stem cells and CHO-K1 cells is reported.

scholarly journals Preparation of optimized lipid-coated calcium phosphate nanoparticles for enhanced in vitro gene delivery to breast cancer cells

2015 ◽  
Vol 3 (33) ◽  
pp. 6805-6812 ◽  
Author(s):  
Jie Tang ◽  
Li Li ◽  
Christopher B. Howard ◽  
Stephen M. Mahler ◽  
Leaf Huang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Calcium Phosphate ◽  
Cell Growth ◽  
Gene Delivery ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cancer Cell Growth ◽  
Calcium Phosphate Nanoparticles ◽  
Transfection Agent

The optimized lipid coated calcium phosphate nanoparticles more efficiently deliver functional siRNA and inhibit the cancer cell growth, in comparison with the commercial transfection agent OligofactamineTM.

pDNA loaded calcium phosphate nanoparticles: highly efficient non-viral vector for gene delivery

2005 ◽  
Vol 288 (1) ◽  
pp. 157-168 ◽  
Author(s):  
Savita Bisht ◽  
Gajadhar Bhakta ◽  
Susmita Mitra ◽  
Amarnath Maitra
Keyword(s):  
Calcium Phosphate ◽  
Gene Delivery ◽  
Viral Vector ◽  
Highly Efficient ◽  
Calcium Phosphate Nanoparticles

scholarly journals Photophysical characterization of Dye-Encapsulated Calcium Phosphate Nanoparticles

2009 ◽  
Vol 96 (3) ◽  
pp. 400a
Author(s):  
Hari S. Muddana ◽  
Thomas T. Morgan ◽  
Tristan Tabouillot ◽  
Erhan I. Altinoglu ◽  
James H. Adair ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Nanoparticles

Gene delivery with bisphosphonate-stabilized calcium phosphate nanoparticles

2011 ◽  
Vol 150 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Elisabeth V. Giger ◽  
Josep Puigmartí-Luis ◽  
Rahel Schlatter ◽  
Bastien Castagner ◽  
Petra S. Dittrich ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Gene Delivery ◽  
Calcium Phosphate Nanoparticles
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