scholarly journals Triple PLGA/PCL Scaffold Modification Including Silver Impregnation, Collagen Coating, and Electrospinning Significantly Improve Biocompatibility, Antimicrobial, and Osteogenic Properties for Orofacial Tissue Regeneration

2019 ◽  
Vol 11 (41) ◽  
pp. 37381-37396 ◽  
Author(s):  
Yunzhu Qian ◽  
Xuefeng Zhou ◽  
Feimin Zhang ◽  
Thomas G. H. Diekwisch ◽  
Xianghong Luan ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Silver Impregnation ◽  
Collagen Coating

A rapid silver-impregnation technique on ultra-thin sections for Electron Microscopy

1993 ◽  
Vol 51 ◽  
pp. 242-243
Author(s):  
K. Chien ◽  
R.C. Heusser ◽  
M.L. Jones ◽  
R.L. Van de Velde
Keyword(s):  
Electron Microscopy ◽  
Silver Nitrate ◽  
Silver Impregnation ◽  
Sodium Borate ◽  
Renal Diseases ◽  
Distilled Water ◽  
Thin Sections ◽  
Impregnation Technique ◽  
Centrifuge Tube ◽  
Simplified Procedure

Silver impregnation techniques have been used for the demonstration of the complex carbohydrates in electron microscopy. However, the silver stains were believed to be technically sensitive and time consumming to perform. Currently, due to the need to more specifically evaluate immune complex for localization in certain renal diseases, a simplified procedure in conjunction with the use of the microwave has been developed and applied to renal and other biopsies. The procedure is as follows:Preparation of silver methenamine solution:1. 15ml graduated, clear polystyrene centrifuge tube (Falcon, No. 2099) was rinsed once with distilled water.2. 3% hexamethylene tetramine (methenamine) was added into the centrifuge tube to the 6ml mark.3. 3% silver nitrate was added slowly to the methenamine to the 7ml mark while agitating. (Solution will instantly turn milky in color and then clear rapidly by mixing. No precipitate should be formed).4. 2% sodium borate was added to the solution to the 8ml mark, mixed and centrifuged before use.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

192: Tubularized Urethral Replacement: What is the Maximum Distance for Normal Tissue Regeneration?

2004 ◽  
Vol 171 (4S) ◽  
pp. 51-51
Author(s):  
Roger E. De Filippo ◽  
Hans G. Pohl ◽  
James J. Yoo ◽  
Anthony Atala
Keyword(s):  
Tissue Regeneration ◽  
Normal Tissue ◽  
Maximum Distance

Metalloproteinases as mediators of tissue regeneration in the peripheral nerve: an important role for metalloproteinase-2

2007 ◽  
Vol 34 (S 2) ◽  
Author(s):  
A Köhne ◽  
HC Lehmann ◽  
O Kiehl ◽  
G Meyer zu Hörste ◽  
HP Hartung ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Tissue Regeneration

EZH2 regulates responses of endothelial cells under hypoxia and during tissue regeneration following limb ischemia

2014 ◽  
Author(s):  
Tijana Mitic ◽  
Micol Marchetti ◽  
Marco Meloni ◽  
Andrea Caporali ◽  
Costanza Emanueli
Keyword(s):  
Endothelial Cells ◽  
Tissue Regeneration ◽  
Limb Ischemia

STRATEGY OF TREATMENT OF PATIENTS WITH ENDODONT-PARODONTAL DISEASES WITH APPLICATION OF DIRECTIONAL TISSUE REGENERATION

2017 ◽  
Vol 19 (8) ◽  
pp. 27-31
Author(s):  
P.V. Moroz ◽  
◽  
S.V. Ataeva ◽  
Y.A. Birkina ◽  
◽  
...  
Keyword(s):  
Tissue Regeneration
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