Fabrication of Topographically Controlled Electrospun Scaffolds to Mimic the Stem Cell Microenvironment in the Dermal-Epidermal Junction

2021 ◽  
Author(s):  
David H. Ramos-Rodriguez ◽  
Sheila MacNeil ◽  
Frederik Claeyssens ◽  
Ilida Ortega Asencio
Keyword(s):  
Stem Cell ◽  
Cell Microenvironment ◽  
Electrospun Scaffolds

The cancer stem cell microenvironment and anti-cancer therapy

2009 ◽  
Vol 85 (11) ◽  
pp. 955-962 ◽  
Author(s):  
Veerander P. S. Ghotra ◽  
Jordi C. Puigvert ◽  
Erik H. J. Danen
Keyword(s):  
Stem Cell ◽  
Cancer Therapy ◽  
Cancer Stem Cell ◽  
Cell Microenvironment ◽  
Anti Cancer

scholarly journals Organizational metrics of interchromatin speckle factor domains: integrative classifier for stem cell adhesion & lineage signaling

2015 ◽  
Vol 7 (4) ◽  
pp. 435-446 ◽  
Author(s):  
Sebastián L. Vega ◽  
Anandika Dhaliwal ◽  
Varun Arvind ◽  
Parth J. Patel ◽  
Nick R. M. Beijer ◽  
...  
Keyword(s):  
Content Analysis ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Nuclear Protein ◽  
Cell Lineage ◽  
Lineage Commitment ◽  
Cell Microenvironment ◽  
Stem Cell Lineage ◽  
High Content Analysis

Timely classification of stem cell lineage commitment in response to cell–microenvironment interactions using high content analysis of sub-nuclear protein organization.

scholarly journals Comparative characteristics of bone marrow cell composition, stroma, and trabecular bone in allogenic hematopoietic stem cell transplantation in patients with primary myelofibrosis

2021 ◽  
Vol 66 (1) ◽  
pp. 68-78
Author(s):  
D. I. Chebotarev ◽  
A. M. Kovrigina ◽  
A. L. Melikyan ◽  
L. A. Kuzmina
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Trabecular Bone ◽  
Bone Remodelling ◽  
Primary Myelofibrosis ◽  
Trephine Biopsy ◽  
Cell Composition ◽  
Cell Microenvironment ◽  
Haematopoietic Tissue

Introduction. Primary myelofibrosis (PMF) is a clonal disease violating the cell composition, histological topography and stroma in bone marrow (BM). Allogeneic haematopoietic stem cell transplantation (allo-HSCT) is a curative therapy in PMF.Aim — description of change in the haematopoietic tissue cell composition and stroma, as well as in trabecular bone in allo-HSCT patients with fibrotic PMF.Materials and methods. We studies 24 trephine biopsy samples from nine PMF patients with allo-HSCT at the intervals: I — 1 month prior to, II — past 1–3 months and III — past 4–6 months from allo-HSCT. BM trephine biopsy slides were prepared in a standard histological assay with haematoxylin—eosin and additional staining with Gomori’s silver and Masson’s trichrome. Morphological change was evaluated in reticulin and collagen stroma, bone trabeculae, cellularity and topography of haematopoietic tissue.Results. The BM trephine biopsies of interval I were morphologically distinguished in three types by haematopoietic cellularity, stromal and trabecular sclerotic change. Post-transplant intervals II and III (3–6 months after allo-HSCT) did not reveal these types but showed an evident myelofibrosis and osteosclerosis reduction and signs of a restoring bone remodelling cycle. Myelopoietic lineages recovered in stages: the erythroid germ restored in three, granulocytic — in six months, and megakaryocytic cellularity did not fully recover in six months. Myelopoietic cellularity recovery outpaced blood recovery, which may be due to induced myelodysplasia or disruption of stromal niches.Conclusion. Allo-HSCT leads to the disappearance of PMF-pathognomonic BM morphology reflecting a histological remission. The reduction of myelofibrosis and osteosclerosis and normalisation of the trabecular bone remodelling cycle in post-transplant periods indicates an impact of cell microenvironment on PMF pathogenesis and warrants research into the composition and histological topography of cell microenvironment in PMF.

The chemotaxis study of adipose derived stem cells (ADSCs) to adenoid cystic carcinoma (ACC) cells.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e17000-e17000
Author(s):  
Li Hua ◽  
Han Zhengxue
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Adenoid Cystic Carcinoma ◽  
Culture Supernatant ◽  
Adipose Derived Stem Cells ◽  
Logarithmic Growth Phase ◽  
Cell Microenvironment ◽  
Adenoid Cystic ◽  
Lower Chamber ◽  
Logarithmic Growth

e17000 Background: Adenoid cystic carcinoma (ACC) has some properties such as neurotropic, angiotropic and early metastasis, recurrence and metastasis is the leading cause of poor prognosis in sACC. The current theory is that the invasion and metastasis were related to tumor stem cells, and the stem cell microenvironment play an important role in this process. According to the stem cell theory, we hypothesized that normal tissue stem cells may also produce chemotactic migration to tumor stem cell microenvironment, therefore this research was to explore the possibility of adipose derived stem cells as a therapy vector targeted to the microenvironment of ACC. Methods: Conventionally culture ACC cells to logarithmic growth phase, then collected them and cultured in serum-free medium. After 48 h, the culture supernatant was collected, filtered and prewarmed, and then added to each well (24-well plate, the lower chamber of the transwell chamber), 600-800 ul for each. Using the same method, the culture supernatant of T293 cells were joined into the lower chamber as a control. Then, the transwell membrane was gently placed into the well, and 100 ~ 150μl suspension of adipose derived stem cells in the logarithmic growth phase (serum-free media) were added into the upper chamber. After cultured under routine conditions for 24 hours, the transwell chamber were removed , fixed and stained. Under bright field microscope, cells invasived and attached to the transwell membrane were observed and counted, results were statistically analyzed by student test. Results: Selecte five high-power microscopic field randomly, counted the number of adipose derived stem cells migrated to transwell membrane of different cell lines. Results showed that migrated cell number of SACC-LM group was 64.3 ± 7.6, the number of SACC-83 group was 52.3 ± 6.1, both higher than the T293 group (31.8 ± 6.3), and the difference between them was statistically significant (p <0.05). Conclusions: Adipose derived stem cells have obvious chemotaxis to adenoid cystic carcinoma cell culture supernatants, so we can propose that adipose derived stem cells has the possibility and feasibiliy of being used as a targeted therapy vectors for ACC.

scholarly journals Engineering the CNS stem cell microenvironment

2009 ◽  
Vol 4 (6) ◽  
pp. 865-877 ◽  
Author(s):  
Cicely A Williams ◽  
Erin B Lavik
Keyword(s):  
Stem Cell ◽  
Cell Microenvironment

scholarly journals First steps to define murine amniotic fluid stem cell microenvironment

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
E. Bertin ◽  
M. Piccoli ◽  
C. Franzin ◽  
G. Spiro ◽  
S. Donà ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Cell Microenvironment ◽  
Amniotic Fluid Stem Cell
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