The chemotaxis study of adipose derived stem cells (ADSCs) to adenoid cystic carcinoma (ACC) cells.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e17000-e17000
Author(s):  
Li Hua ◽  
Han Zhengxue
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Adenoid Cystic Carcinoma ◽  
Culture Supernatant ◽  
Adipose Derived Stem Cells ◽  
Logarithmic Growth Phase ◽  
Cell Microenvironment ◽  
Adenoid Cystic ◽  
Lower Chamber ◽  
Logarithmic Growth

e17000 Background: Adenoid cystic carcinoma (ACC) has some properties such as neurotropic, angiotropic and early metastasis, recurrence and metastasis is the leading cause of poor prognosis in sACC. The current theory is that the invasion and metastasis were related to tumor stem cells, and the stem cell microenvironment play an important role in this process. According to the stem cell theory, we hypothesized that normal tissue stem cells may also produce chemotactic migration to tumor stem cell microenvironment, therefore this research was to explore the possibility of adipose derived stem cells as a therapy vector targeted to the microenvironment of ACC. Methods: Conventionally culture ACC cells to logarithmic growth phase, then collected them and cultured in serum-free medium. After 48 h, the culture supernatant was collected, filtered and prewarmed, and then added to each well (24-well plate, the lower chamber of the transwell chamber), 600-800 ul for each. Using the same method, the culture supernatant of T293 cells were joined into the lower chamber as a control. Then, the transwell membrane was gently placed into the well, and 100 ~ 150μl suspension of adipose derived stem cells in the logarithmic growth phase (serum-free media) were added into the upper chamber. After cultured under routine conditions for 24 hours, the transwell chamber were removed , fixed and stained. Under bright field microscope, cells invasived and attached to the transwell membrane were observed and counted, results were statistically analyzed by student test. Results: Selecte five high-power microscopic field randomly, counted the number of adipose derived stem cells migrated to transwell membrane of different cell lines. Results showed that migrated cell number of SACC-LM group was 64.3 ± 7.6, the number of SACC-83 group was 52.3 ± 6.1, both higher than the T293 group (31.8 ± 6.3), and the difference between them was statistically significant (p <0.05). Conclusions: Adipose derived stem cells have obvious chemotaxis to adenoid cystic carcinoma cell culture supernatants, so we can propose that adipose derived stem cells has the possibility and feasibiliy of being used as a targeted therapy vectors for ACC.

Application of Nanomaterials in Regulating the Fate of Adipose-derived Stem Cells

2021 ◽  
Vol 16 (1) ◽  
pp. 3-13
Author(s):  
Lang Wang ◽  
Yong Li ◽  
Maorui Zhang ◽  
Kui Huang ◽  
Shuanglin Peng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Research ◽  
Adult Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Proliferation And Differentiation ◽  
Recent Developments ◽  
Good Biocompatibility ◽  
Regulatory Effects ◽  
New Treatment

Adipose-derived stem cells are adult stem cells which are easy to obtain and multi-potent. Stem-cell therapy has become a promising new treatment for many diseases, and plays an increasingly important role in the field of tissue repair, regeneration and reconstruction. The physicochemical properties of the extracellular microenvironment contribute to the regulation of the fate of stem cells. Nanomaterials have stable particle size, large specific surface area and good biocompatibility, which has led them being recognized as having broad application prospects in the field of biomedicine. In this paper, we review recent developments of nanomaterials in adipose-derived stem cell research. Taken together, the current literature indicates that nanomaterials can regulate the proliferation and differentiation of adipose-derived stem cells. However, the properties and regulatory effects of nanomaterials can vary widely depending on their composition. This review aims to provide a comprehensive guide for future stem-cell research on the use of nanomaterials.

Elimination of Reperfusion-Induced Microcirculatory Alterations In Vivo by Adipose-Derived Stem Cell Supernatant without Adipose-Derived Stem Cells

2015 ◽  
Vol 135 (4) ◽  
pp. 1056-1064 ◽  
Author(s):  
Wei Z. Wang ◽  
Xin-Hua Fang ◽  
Shelley J. Williams ◽  
Linda L. Stephenson ◽  
Richard C. Baynosa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Adipose Derived Stem Cells ◽  
Adipose Derived Stem Cell ◽  
Cell Supernatant

scholarly journals Multi-modal imaging probe for assessing the efficiency of stem cell delivery to orthotopic breast tumours

Nanoscale ◽  
2020 ◽  
Vol 12 (31) ◽  
pp. 16570-16585 ◽  
Author(s):  
May Zaw Thin ◽  
Helen Allan ◽  
Robin Bofinger ◽  
Tomas D. Kostelec ◽  
Simon Guillaume ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Delivery ◽  
Adipose Derived Stem Cells ◽  
Breast Tumours ◽  
Imaging Probe ◽  
Stem Cell Delivery

Illustration of adipose-derived stem cells with tri-modal imaging capabilities for evaluating the efficiency of cell delivery to tumours.

scholarly journals Use of adipose-derived stem cells in lymphatic tissue engineering and regeneration

2021 ◽  
Vol 48 (5) ◽  
pp. 559-567
Author(s):  
Antonio Jorge Forte ◽  
Daniel Boczar ◽  
Rachel Sarabia-Estrada ◽  
Maria T. Huayllani ◽  
Francisco R. Avila ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Systematic Review ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Factor ◽  
Cochrane Central Register ◽  
Adipose Derived Stem Cells ◽  
Lymphatic Tissue ◽  
Factor C

The potential to differentiate into different cell lines, added to the easy and cost-effective method of extraction, makes adipose-derived stem cells (ADSCs) an object of interest in lymphedema treatment. Our study’s goal was to conduct a comprehensive systematic review of the use of ADSCs in lymphatic tissue engineering and regeneration. On July 23, 2019, using PubMed/MEDLINE, Cochrane Clinical Answers, Cochrane Central Register of Controlled Trials, and Embase databases, we conducted a systematic review of published literature on the use of ADSCs in lymphatic tissue engineering and regeneration. There were no language or time frame limitations, and the following search strategy was applied: ((Adipose stem cell) OR Adipose-derived stem cell)) AND ((Lymphedema) OR Breast Cancer Lymphedema). Only original research manuscripts were included. Fourteen studies fulfilled the inclusion criteria. Eleven studies were experimental (in vitro or in vivo in animals), and only three were clinical. Publications on the topic demonstrated that ADSCs promote lymphangiogenesis, and its effect could be enhanced by modulation of vascular endothelial growth factor-C, interleukin-7, prospero homeobox protein 1, and transforming growth factor-β1. Pilot clinical studies included 11 patients with breast cancer-related lymphedema, and no significant side effects were present at 12-month follow-up. Literature on the use of ADSCs in lymphatic tissue engineering and regeneration demonstrated promising data. Clinical evidence is still in its infancy, but the scientific community agrees that ADSCs can be useful in regenerative lymphangiogenesis. Data collected in this review indicate that unprecedented advances in lymphedema treatment can be anticipated in the upcoming years.

scholarly journals A and alpha supernatant pretreatment of Saccharomyces cerevisiae cells affects both the kinetics and efficiency of mating.

1982 ◽  
Vol 2 (8) ◽  
pp. 897-903 ◽  
Author(s):  
E P Sena
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Fusion ◽  
Large Scale ◽  
Culture Supernatant ◽  
Treatment Procedure ◽  
Logarithmic Growth Phase ◽  
Future Studies ◽  
Mating Efficiency ◽  
Potential Applications ◽  
Logarithmic Growth

The effects of culture supernatant treatment on subsequent matings between pretreated a and alpha Saccharomyces cerevisiae cells were studied. For each experiment, pairs of a and alpha [rho+] or [rho- rho0] cells in the logarithmic growth phase in defined minimal medium were pretreated for a total of 15 min (by exchanging their cell-free supernatants or by mixing samples of a and alpha cell cultures) and then mated in defined minimal (YNB) or enriched (YEP) liquid medium. All pretreated cells, regardless of treatment procedure, initiated cell fusion 15 to 35 min faster than did their nontreated counterparts. In all cases, pretreated cells mated 8 to 20% more efficiently than did nonpretreated ones. Regardless of the strains, the hierarchy of mating efficiency was always treated YEP greater than untreated YEP greater than treated YNB greater than untreated YNB. The cell fusion kinetics in alpha [rho+] X a [rho-] crosses were most affected by pretreatment (delta 30 to 35 min), whereas [rho+] X [rho+] crosses were least affected (delta 15 min). These results are discussed in relation to the functions known for a and alpha pheromones. The successful pretreatment regimes were used to design new rapid and efficient techniques for mating YNB-grown log-phase cells in either YNB or YEP liquid media. These techniques can be used for small- or large-scale mating, and because of their inherent media flexibility, they have many potential applications to future studies on mating-specific or intrazygotic phenomena.

Adipose-derived stem cells enhance human breast cancer growth and cancer stem cell-like properties through adipsin

Oncogene ◽  
2018 ◽  
Vol 38 (6) ◽  
pp. 767-779 ◽  
Author(s):  
Hideaki Goto ◽  
Yohei Shimono ◽  
Yohei Funakoshi ◽  
Yoshinori Imamura ◽  
Masanori Toyoda ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Human Breast Cancer ◽  
Cancer Growth ◽  
Adipose Derived Stem Cells ◽  
Human Breast ◽  
Breast Cancer Growth

A and alpha supernatant pretreatment of Saccharomyces cerevisiae cells affects both the kinetics and efficiency of mating

1982 ◽  
Vol 2 (8) ◽  
pp. 897-903
Author(s):  
E P Sena
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Fusion ◽  
Large Scale ◽  
Culture Supernatant ◽  
Treatment Procedure ◽  
Logarithmic Growth Phase ◽  
Future Studies ◽  
Mating Efficiency ◽  
Potential Applications ◽  
Logarithmic Growth

The effects of culture supernatant treatment on subsequent matings between pretreated a and alpha Saccharomyces cerevisiae cells were studied. For each experiment, pairs of a and alpha [rho+] or [rho- rho0] cells in the logarithmic growth phase in defined minimal medium were pretreated for a total of 15 min (by exchanging their cell-free supernatants or by mixing samples of a and alpha cell cultures) and then mated in defined minimal (YNB) or enriched (YEP) liquid medium. All pretreated cells, regardless of treatment procedure, initiated cell fusion 15 to 35 min faster than did their nontreated counterparts. In all cases, pretreated cells mated 8 to 20% more efficiently than did nonpretreated ones. Regardless of the strains, the hierarchy of mating efficiency was always treated YEP greater than untreated YEP greater than treated YNB greater than untreated YNB. The cell fusion kinetics in alpha [rho+] X a [rho-] crosses were most affected by pretreatment (delta 30 to 35 min), whereas [rho+] X [rho+] crosses were least affected (delta 15 min). These results are discussed in relation to the functions known for a and alpha pheromones. The successful pretreatment regimes were used to design new rapid and efficient techniques for mating YNB-grown log-phase cells in either YNB or YEP liquid media. These techniques can be used for small- or large-scale mating, and because of their inherent media flexibility, they have many potential applications to future studies on mating-specific or intrazygotic phenomena.

scholarly journals Improvement of systemic lupus erythematosus in dogs with canine adipose-derived stem cells

2019 ◽  
Vol 64 (No. 10) ◽  
pp. 462-466
Author(s):  
M Ko ◽  
TH Kim ◽  
Y Kim ◽  
D Kim ◽  
JO Ahn ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Stem Cells ◽  
Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Lupus Erythematosus ◽  
Adipose Derived Stem Cells ◽  
Systemic Lupus ◽  
Hind Limbs ◽  
Life Threatening

A 6-year-old, intact female, Maltese presented with limited movement of the hind limbs and intermittent pruritus for three months. The patient was diagnosed with systemic lupus erythematosus. Conventional immunosuppressive therapy was attempted for 70 days; however, the patient still suffered from life-threatening pancreatitis and hepatopathy. Therefore, we tried canine adipose-derived mesenchymal stem cells for immunomodulation and liver protection. After 6-months of the stem cell therapy, the patient’s walking and hepatopathy improved. These findings indicate that stem cell therapy may be another option for systemic lupus erythematosus in dogs.

200 TRANSPLANTATION AND IN VIVO DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS IN SWINE

2006 ◽  
Vol 18 (2) ◽  
pp. 208 ◽  
Author(s):  
A. S. Lima ◽  
S. A. Malusky ◽  
M. R. B. Mello ◽  
S. J. Lane ◽  
J. R. Rivera ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Stem Cell ◽  
Cellular Response ◽  
Agricultural Research ◽  
Primary Concern ◽  
Adipose Derived Stem Cells ◽  
Subcutaneous Adipose

A primary concern in stem cell biology is that observations made in vitro may be an artifact of the in vitro culture environment. In vitro derived stem cells can be implanted into the environment from which they are derived so that their response to physiological conditions may be observed. Several important cellular characteristics need to be examined following the cell's reintroduction to the in vivo environment, including the potential for differentiation, proliferative ability, and life span. Studying implanted stem cells will assist in determining the potential for stem cell use in clinical therapies and provide further understanding of the role adult stem cells have in the adult body. Currently, the scientific literature is lacking a detailed description of the cellular response of adipose-derived stem cells (ADSCs) reintroduced to their exact tissue of origin. Thus, the aim of this study was to evaluate porcine ADSC growth in vivo and to analyze cell differentiation in vivo following injection of undifferentiated ADSCs into subcutaneous fat. Subcutaneous adipose tissue was isolated from the back fat of male pigs (11 months of age) and digested with 0.075% collagenase at 37�C for 90 min. The digested tissue was centrifuged at 200g for 10 min to obtain a cell pellet. The pellet was re-suspended with DMEM and the ADSCs were plated onto 75 cm2 flasks (5000-10 000 cells per cm2) and cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% gentamicin. Passage 3 ADSCs were labeled with fluorescent dye (PKH26; Sigma, St. Louis, MO, USA) and sorted by flow cytometry. After sorting, positive cells were washed and re-suspended in culture medium. For transplantation, 100 �L of cell suspension in DMEM containing one of four cell concentrations (0 (control); 30 000; 300 000; and 900 000 cells) were placed in a 1-mL syringe and injected into the subcutaneous back fat of recipient pigs (n = 2). Each pig had previously been tattooed with 12 13 � 13 squares to mark injection sites. The treatments were replicated three times within each animal. Two and three weeks after transplantation, animals were euthanized, the back fat containing the transplantation site was harvested, and the cells were disaggregated as described above. The buoyant adipocytes and pelleted ADSCs cells were then analyzed by flow cytometry. The results indicated that there were dose- and time-dependent increases in labeled ADSCs and labeled adipocytes in the fat samples with increasing cell number (from 0 to 300 000 cells). There was, however, a decrease in labeled ADSCs at the 900 000-cell dose, which is likely due to excess cells being transplanted or an immune reaction. Both of these aspects are currently being evaluated. In conclusion, undifferentiated ADSCs from swine can be isolated from and returned to the subcutaneous adipose layer and differentiate into mature adipocytes. This work was supported by the Council for Food and Agricultural Research (C-FAR) Sentinel Program, University of Illinois.

scholarly journals Tuning Adipogenic Differentiation in ADSCs by Metformin and Vitamin D: Involvement of miRNAs

2020 ◽  
Vol 21 (17) ◽  
pp. 6181
Author(s):  
Sara Cruciani ◽  
Giuseppe Garroni ◽  
Francesca Balzano ◽  
Renzo Pala ◽  
Emanuela Bellu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vitamin D ◽  
Stem Cell ◽  
Cell Fate ◽  
Adipogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Vitamin D Metabolism ◽  
Stem Cell Fate ◽  
Cellular Phenotypes

Fat tissue represents an important source of adipose-derived stem cells (ADSCs), which can differentiate towards several phenotypes under certain stimuli. Definite molecules as vitamin D are able to influence stem cell fate, acting on the expression of specific genes. In addition, miRNAs are important modulating factors in obesity and numerous diseases. We previously identified specific conditioned media able to commit stem cells towards defined cellular phenotypes. In the present paper, we aimed at evaluating the role of metformin on ADSCs differentiation. In particular, ADSCs were cultured in a specific adipogenic conditioned medium (MD), in the presence of metformin, alone or in combination with vitamin D. Our results showed that the combination of the two compounds is able to counteract the appearance of an adipogenic phenotype, indicating a feedforward regulation on vitamin D metabolism by metformin, acting on CYP27B1 and CYP3A4. We then evaluated the role of specific epigenetic modulating genes and miRNAs in controlling stem cell adipogenesis. The combination of the two molecules was able to influence stem cell fate, by modulating the adipogenic phenotype, suggesting their possible application in clinical practice in counteracting uncontrolled lipogenesis and obesity-related diseases.

Sign in / Sign up

Export Citation Format

Share Document