A Multimodal Ca(II) Responsive Near IR-MR Contrast Agent Exhibiting High Cellular Uptake

2020 ◽  
Vol 15 (2) ◽  
pp. 334-341
Author(s):  
Casey J. Adams ◽  
Ruby Krueger ◽  
Thomas J. Meade
Keyword(s):  
Contrast Agent ◽  
Cellular Uptake ◽  
Near Ir

scholarly journals Investigation of Specific Targeting of Triptorelin-Conjugated Dextran-Coated Magnetite Nanoparticles as a Targeted Probe in GnRH+ Cancer Cells in MRI

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Milad Yousefvand ◽  
Zahra Mohammadi ◽  
Farzaneh Ghorbani ◽  
Rasoul Irajirad ◽  
Hormoz Abedi ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Tumor Targeting ◽  
Tumor Imaging ◽  
Superparamagnetic Iron Oxide ◽  
Gnrh Receptors ◽  
Imaging Probes ◽  
Magnetic Resonance Imaging Mri ◽  
Targeted Contrast Agent

In recent years, the conjugation of superparamagnetic iron oxide nanoparticles (SPIONs), as tumor-imaging probes for magnetic resonance imaging (MRI), with tumor targeting peptides possesses promising advantages for specific delivery of MRI agents. The objective of the current study was to design a targeted contrast agent for MRI based on Fe3O4 nanoparticles conjugated triptorelin (SPION@triptorelin), which has a great affinity to the GnRH receptors. The SPIONs-coated carboxymethyl dextran (SPION@CMD) conjugated triptorelin (SPION@CMD@triptorelin) were synthesized using coprecipitation method and characterized by DLS, TEM, XRD, FTIR, Zeta, and VSM techniques. The relaxivities of synthetized formulations were then calculated using a 1.5 Tesla clinical magnetic field. MRI, quantitative cellular uptake, and cytotoxicity level of them were estimated. The characterization results confirmed that the formation of SPION@CMD@triptorelin has been conjugated with a suitable size. Our results demonstrated the lack of cellular cytotoxicity of SPION@CMD@triptorelin, and it could increase the cellular uptake of SPIONs to MDA-MB-231 cancer cells 6.50-fold greater than to SPION@CMD at the concentration of 75 μM. The relaxivity calculations for SPION@CMD@triptorelin showed a suitable r2 and r2/r1 with values of 31.75 mM−1·s−1 and 10.26, respectively. Our findings confirm that triptorelin-targeted SPIONs could provide a T2-weighted probe contrast agent that has the great potential for the diagnosis of GnRH-positive cancer in MRI.

scholarly journals Triazole-based osmium(ii) complexes displaying red/near-IR luminescence: antimicrobial activity and super-resolution imaging

2020 ◽  
Vol 11 (33) ◽  
pp. 8928-8935
Author(s):  
Kirsty L. Smitten ◽  
Paul A. Scattergood ◽  
Charlotte Kiker ◽  
Jim A. Thomas ◽  
Paul I. P. Elliott
Keyword(s):  
Antimicrobial Activity ◽  
Cellular Uptake ◽  
Super Resolution ◽  
Methicillin Resistant ◽  
Luminescence Imaging ◽  
Near Ir ◽  
Resolution Imaging

Cellular uptake, luminescence imaging and antimicrobial activity of facial and meridional isomers of Os(ii) triazole-based complexes against methicillin-resistant S. aureus, MRSA.

scholarly journals Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging

2015 ◽  
Vol 137 (28) ◽  
pp. 9108-9116 ◽  
Author(s):  
Victoria S. R. Harrison ◽  
Christiane E. Carney ◽  
Keith W. MacRenaris ◽  
Emily A. Waters ◽  
Thomas J. Meade
Keyword(s):  
Contrast Agent ◽  
In Vivo Imaging ◽  
Near Ir

scholarly journals Cellular uptake and cytotoxicity of a near-IR fluorescent corrole–TiO2 nanoconjugate

2014 ◽  
Vol 140 ◽  
pp. 39-44 ◽  
Author(s):  
Carl M. Blumenfeld ◽  
Bryce F. Sadtler ◽  
G. Esteban Fernandez ◽  
Lily Dara ◽  
Cathie Nguyen ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Near Ir

scholarly journals Polymer-Peptide Modified Gold Nanorods to Improve Cell Conjugation and Cell labelling for Stem Cells Photoacoustic Imaging

Diagnostics ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1196
Author(s):  
Dina Salah ◽  
Farahat S. Moghanm ◽  
Muhammad Arshad ◽  
Abdulaziz A. Alanazi ◽  
Salman latif ◽  
...  
Keyword(s):  
Stem Cells ◽  
Contrast Agent ◽  
Surface Plasmon ◽  
Cellular Uptake ◽  
Gold Nanorods ◽  
Cell Tracking ◽  
Photoacoustic Imaging ◽  
Optical Measurements ◽  
Peptide Sequence ◽  
Ammonium Bromide

The use of gold nanorods (GNRs) as a contrast agent in bioimaging and cell tracking has numerous advantages, primarily due to the unique optical properties of gold nanorods which allow for the use of infrared regions when imaging. Owing to their unique geometry, Au NRs exhibit surface plasmon modes in the near-infrared wavelength range, which is ideal for carrying out optical measurements in biological fluids and tissue. Gold nanorod functionalization is essential, since the Cetyltrimethyl ammonium bromide CTAB gold nanorods are toxic, and for further in vitro and in vivo experiments the nanorods should be functionalized to become optically stable and biocompatible. In the present study, gold nanorods with an longitudinal surface plasmon resonance (LSPR) position around 800 nm were synthesized in order to be used for photoacoustic imaging applications for stem cell tracking. The gold nanorods were functionalized using both thiolated poly (ethylene glycol) (PEG) to stabilize the gold nanorods surface and a CALNN–TAT peptide sequence. Both ligands were attached to the gold nanorods through an Au–sulfur bond. CALNN–TAT is known as a cell penetrating peptide which ensures endocytosis of the gold nanorods inside the mesenchymal stem cells of mice (MSCD1). Surface modifications of gold nanorods were achieved using optical spectroscopy (UV–VIS), electron microscopy (TEM), zeta-potential, and FTIR. Gold nanorods were incubated in MSCD1 in order to achieve a cellular uptake that was characterized by a transmission electron microscope (TEM). For photoacoustic imaging, Multi-Spectral Optoacoustic Tomography (MSOT) was used. The results demonstrated good cellular uptake for PEG–CALNN–TAT GNRs and the successful use of modified gold nanorods as both a contrast agent in photoacoustic imaging and as a novel tracking bioimaging technique.

Near-IR/Visible-Emitting Thiophenyl-Based Ru(II) Complexes: Efficient Photodynamic Therapy, Cellular Uptake, and DNA Binding

2019 ◽  
Vol 58 (20) ◽  
pp. 14244-14259 ◽  
Author(s):  
Si-Qi Zhang ◽  
Ting-Ting Meng ◽  
Jia Li ◽  
Fan Hong ◽  
Jin Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Dna Binding ◽  
Cellular Uptake ◽  
Near Ir

Manganese(II) Texaphyrin: A Paramagnetic Photoacoustic Contrast Agent Activated by Near-IR Light

2020 ◽  
Vol 142 (38) ◽  
pp. 16156-16160
Author(s):  
Yaguang Ren ◽  
Adam C. Sedgwick ◽  
Jingqin Chen ◽  
Gregory Thiabaud ◽  
Calvin V. Chau ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Near Ir ◽  
Ir Light

MR contrast agent composed of cholesterol and peptide nucleic acids: Design, synthesis and cellular uptake

2010 ◽  
Vol 20 (7) ◽  
pp. 2238-2241 ◽  
Author(s):  
Rajendra Joshi ◽  
Ritu Mishra ◽  
Rolf Pohmann ◽  
Jörn Engelmann
Keyword(s):  
Nucleic Acids ◽  
Contrast Agent ◽  
Cellular Uptake ◽  
Peptide Nucleic Acids ◽  
Design Synthesis
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