Promotion of Neuronal Guidance Growth by Aminated Graphene Oxide via Netrin-1/Deleted in Colorectal Cancer Signaling

Central focus in modern anticancer nanosystems is given to certain types of nanomaterials such as graphene oxide (GO). Its functionalization with polyethylene glycol (PEG) demonstrates high delivery efficiency and controllable release of proteins, bioimaging agents, chemotherapeutics and anticancer drugs. GO–PEG has a good biological safety profile, exhibits high NIR absorbance and capacity in photothermal treatment. To investigate the bioactivity of PEGylated GO NPs in combination with NIR irradiation on colorectal cancer cells we conducted experiments that aim to reveal the molecular mechanisms of action of this nanocarrier, combined with near-infrared light (NIR) on the high invasive Colon26 and the low invasive HT29 colon cancer cell lines. During reaching cancer cells the phototoxicity of GO–PEG is modulated by NIR laser irradiation. We observed that PEGylation of GO nanoparticles has well-pronounced biocompatibility toward colorectal carcinoma cells, besides their different malignant potential and treatment times. This biocompatibility is potentiated when GO–PEG treatment is combined with NIR irradiation, especially for cells cultured and treated for 24 h. The tested bioactivity of GO–PEG in combination with NIR irradiation induced little to no damages in DNA and did not influence the mitochondrial activity. Our findings demonstrate the potential of GO–PEG-based photoactivity as a nanosystem for colorectal cancer treatment.

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Increased Expression of Netrin-1 and Its Deleted in Colorectal Cancer Receptor in Human Diseased Lumbar Intervertebral Disc Compared With Autopsy Control

Spine ◽

10.1097/brs.0b013e31825d4ebc ◽

2012 ◽

Vol 37 (25) ◽

pp. 2074-2081 ◽

Cited By ~ 7

Author(s):

Guoyun Bu ◽

Shuxun Hou ◽

Dongfeng Ren ◽

Ye Wu ◽

Weilin Shang ◽

...

Keyword(s):

Colorectal Cancer ◽

Intervertebral Disc ◽

Lumbar Intervertebral Disc ◽

Deleted In Colorectal Cancer

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The Netrin-1/DCC guidance system: dopamine pathway maturation and psychiatric disorders emerging in adolescence

Molecular Psychiatry ◽

10.1038/s41380-019-0561-7 ◽

2019 ◽

Vol 25 (2) ◽

pp. 297-307 ◽

Cited By ~ 10

Author(s):

Daniel E. Vosberg ◽

Marco Leyton ◽

Cecilia Flores

Keyword(s):

Colorectal Cancer ◽

Substance Use ◽

Nervous System ◽

Axon Guidance ◽

Psychiatric Disorders ◽

Genetic Variants ◽

Guidance System ◽

Laboratory Animals ◽

Compelling Evidence ◽

Deleted In Colorectal Cancer

Abstract Axon guidance molecules direct growing axons toward their targets, assembling the intricate wiring of the nervous system. One of these molecules, Netrin-1, and its receptor, DCC (deleted in colorectal cancer), has profound effects, in laboratory animals, on the adolescent expansion of mesocorticolimbic pathways, particularly dopamine. Now, a rapidly growing literature suggests that (1) these same alterations could occur in humans, and (2) genetic variants in Netrin-1 and DCC are associated with depression, schizophrenia, and substance use. Together, these findings provide compelling evidence that Netrin-1 and DCC influence mesocorticolimbic-related psychopathological states that emerge during adolescence.

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