Abstract
Backgrounds:
Microwave sensitization nanoplatform, integrating multiple functional units for improving tumor selectivity, is of great significance for clinical tumor microwave therapy. Lanthanide europium metal organic framework materials (EuMOF) are expected to be a theranostic nanoplatform owing to its specific luminescent properties and microwave sensitization properties. However, it is difficult to be applied to complex biological systems for EuMOF due to its rapid degradation induced by the solvent molecular and ionic environment. In this work, a luminescent EuMOF nanocomposite (EuMOF@ZIF/AP-PEG, named EZAP) was designed, which brought the multifunctional characteristics of microwave sensitization, fluorescence imaging and drug loading.
Results
Lamellar EuMOF was synthesized by a hydrothermal method. Through the charge adsorption mechanism, the zeolite imidazole framework (ZIF) structure was densely assembled on the surface of EuMOF to realize the protection. Then, through in-situ apatinib drug loading and PEG modification, EZAP nanocomposite was finally obtained. Apatinib (AP) was a kind of chemotherapy drug approved by Food and Drug Administration for clinical use. PEG modification increased long-term circulation of EZAP nanocomposite. The physical and chemical structure and properties of EuMOF@ZIF (EZ) were systematically represented, indicating the successful synthesis of the nanocomposite. The toxic and side effects were negligible at a safe dose. The growth of human liver cancer cells and murine liver cancer cells in vitro was significantly inhibited, and the combined microwave-thermal therapy and chemotherapy in vivo achieved high anti-cancer efficacy. Moreover, EZAP nanocomposite possessed bright red fluorescence, which had good ability for tumor imaging in tumor-bearing mice in vivo.
Conclusion
Therefore, EZAP nanocomposite showed high microwave sensitization, excellent fluorescence properties and good drug loading capacity, establishing a promising theranostic nanoplatform for tumor therapy and fluorescence imaging. This work proposes a unique strategy to design for the first time a multifunctional nanoplatform with lanthanide metal organic frameworks for tumor treatment and diagnosis in the biological application.
Phenylboronic-acid-based Functional Chemical Materials for Fluorescence Imaging and Tumor Therapy
