scholarly journals Lanthanide Europium MOF Nanocomposite as the Theranostic Nanoplatform for Microwave Thermo-Chemotherapy and Fluorescence Imaging

2021 ◽  
Author(s):  
Lirong Zhao ◽  
Wei Zhang ◽  
Qiong Wu ◽  
Changhui Fu ◽  
Xiangling Ren ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Tumor Imaging ◽  
Drug Loading ◽  
Tumor Therapy ◽  
Liver Cancer Cells ◽  
Good Ability ◽  
Metal Organic

Abstract Backgrounds: Microwave sensitization nanoplatform, integrating multiple functional units for improving tumor selectivity, is of great significance for clinical tumor microwave therapy. Lanthanide europium metal organic framework materials (EuMOF) are expected to be a theranostic nanoplatform owing to its specific luminescent properties and microwave sensitization properties. However, it is difficult to be applied to complex biological systems for EuMOF due to its rapid degradation induced by the solvent molecular and ionic environment. In this work, a luminescent EuMOF nanocomposite (EuMOF@ZIF/AP-PEG, named EZAP) was designed, which brought the multifunctional characteristics of microwave sensitization, fluorescence imaging and drug loading. Results Lamellar EuMOF was synthesized by a hydrothermal method. Through the charge adsorption mechanism, the zeolite imidazole framework (ZIF) structure was densely assembled on the surface of EuMOF to realize the protection. Then, through in-situ apatinib drug loading and PEG modification, EZAP nanocomposite was finally obtained. Apatinib (AP) was a kind of chemotherapy drug approved by Food and Drug Administration for clinical use. PEG modification increased long-term circulation of EZAP nanocomposite. The physical and chemical structure and properties of EuMOF@ZIF (EZ) were systematically represented, indicating the successful synthesis of the nanocomposite. The toxic and side effects were negligible at a safe dose. The growth of human liver cancer cells and murine liver cancer cells in vitro was significantly inhibited, and the combined microwave-thermal therapy and chemotherapy in vivo achieved high anti-cancer efficacy. Moreover, EZAP nanocomposite possessed bright red fluorescence, which had good ability for tumor imaging in tumor-bearing mice in vivo. Conclusion Therefore, EZAP nanocomposite showed high microwave sensitization, excellent fluorescence properties and good drug loading capacity, establishing a promising theranostic nanoplatform for tumor therapy and fluorescence imaging. This work proposes a unique strategy to design for the first time a multifunctional nanoplatform with lanthanide metal organic frameworks for tumor treatment and diagnosis in the biological application.

scholarly journals Synergistic Interaction between the HDAC Inhibitor, MPT0E028, and Sorafenib in Liver Cancer Cells In Vitro and In Vivo

2014 ◽  
Vol 20 (5) ◽  
pp. 1274-1287 ◽  
Author(s):  
Chun-Han Chen ◽  
Mei-Chuan Chen ◽  
Jing-Chi Wang ◽  
An-Chi Tsai ◽  
Ching-Shih Chen ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Hdac Inhibitor ◽  
Synergistic Interaction ◽  
Liver Cancer Cells

Growth inhibitory effects of pegylated IFN ?-2b on human liver cancer cells in vitro and in vivo

2006 ◽  
Vol 26 (8) ◽  
pp. 964-975 ◽  
Author(s):  
Hirohisa Yano ◽  
Sachiko Ogasawara ◽  
Seiya Momosaki ◽  
Jun Akiba ◽  
Sakiko Kojiro ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Human Liver ◽  
Inhibitory Effects ◽  
Liver Cancer Cells ◽  
Growth Inhibitory ◽  
Human Liver Cancer ◽  
Human Liver Cancer Cells

The MAP30 protein from bitter gourd (Momordica charantia) seeds promotes apoptosis in liver cancer cells in vitro and in vivo

2012 ◽  
Vol 324 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Evandro Fei Fang ◽  
Chris Zhi Yi Zhang ◽  
Jack Ho Wong ◽  
Jia Yun Shen ◽  
Chuan Hao Li ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Momordica Charantia ◽  
Bitter Gourd ◽  
Liver Cancer Cells

scholarly journals Multi-modal channel cancer chemotherapy by 2D functional gadolinium metal-organic framework

2020 ◽  
Author(s):  
Jiale Xia ◽  
Yumeng Xue ◽  
Bo Lei ◽  
Lingling Xu ◽  
Mingzi Sun ◽  
...  
Keyword(s):  
Cancer Chemotherapy ◽  
Tumor Tissue ◽  
Tumor Imaging ◽  
Metal Organic Framework ◽  
Drug Loading ◽  
High Surface ◽  
Superior Performance ◽  
Metal Organic ◽  
Organic Framework

Abstract Two-dimensional (2D) nanomaterials generally exhibit enhanced physiochemical and biological functions in biomedical applications due to their high surface-to-volume ratio and surface charge. Conventional cancer chemotherapy based on nanomaterials has been hindered by their low drug loading and poor penetration in tumor tissue. To overcome these difficulties, the novel materials systems are urgently needed. Hereby, the lanthanide-based porphyrin metal organic framework (MOF) nanosheets with promising cancer imaging/chemotherapy capacities are fabricated, which displays superior performance in the drug loading and tumor tissue penetration. The biodegradable PPF-Gd nanosheets deliver an ultrahigh drug loading (>1500%) and demonstrate the stable and highly sensitive stimuli-responsive degradation/release for multimodal tumor imaging and cancer chemotherapy. Meanwhile, PPF-Gd NSs also exhibits excellent fluorescence and magnetic resonance imaging capability in vitro and in vivo. Compared to the traditional DOX chemotherapy, the in vivo results confirm the evident suppression of the tumor growth by the PPF-Gd/DOX drug delivery system with negligible side effects. This work further supports the potential of lanthanide-based MOF nanomaterials as biodegradable systems to promote the cancer theranostics technology development in the future.

scholarly journals Composite fatty acid ether amides suppress growth of liver cancer cells in vitro and in an in vivo allograft mouse model

2013 ◽  
Vol 36 (3) ◽  
pp. 247-257 ◽  
Author(s):  
Mengde Cao ◽  
Victor Prima ◽  
David Nelson ◽  
Stanislav Svetlov
Keyword(s):  
Fatty Acid ◽  
Liver Cancer ◽  
Mouse Model ◽  
Cancer Cells ◽  
Liver Cancer Cells

Carbidopa is an activator of aryl hydrocarbon receptor with potential for cancer therapy

2017 ◽  
Vol 474 (20) ◽  
pp. 3391-3402 ◽  
Author(s):  
Jiro Ogura ◽  
Seiji Miyauchi ◽  
Kazumi Shimono ◽  
Shengping Yang ◽  
Sathisha Gonchigar ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Liver Cancer ◽  
Aryl Hydrocarbon Receptor ◽  
Cancer Cells ◽  
Inhibitory Effect ◽  
Anticancer Effect ◽  
Liver Cancer Cells ◽  
Aryl Hydrocarbon

Carbidopa is used with l-DOPA (l-3,4-dihydroxyphenylalanine) to treat Parkinson's disease (PD). PD patients exhibit lower incidence of most cancers including pancreatic cancer, but with the notable exception of melanoma. The decreased cancer incidence is not due to l-DOPA; however, the relevance of Carbidopa to this phenomenon has not been investigated. Here, we tested the hypothesis that Carbidopa, independent of l-DOPA, might elicit an anticancer effect. Carbidopa inhibited pancreatic cancer cell proliferation both in vitro and in vivo. Based on structural similarity with phenylhydrazine, an inhibitor of indoleamine-2,3-dioxygenase-1 (IDO1), we predicted that Carbidopa might also inhibit IDO1, thus providing a molecular basis for its anticancer effect. The inhibitory effect was confirmed using human recombinant IDO1. To demonstrate the inhibition in intact cells, AhR (aryl hydrocarbon receptor) activity was monitored as readout for IDO1-mediated generation of the endogenous AhR agonist kynurenine in pancreatic and liver cancer cells. Surprisingly, Carbidopa did not inhibit but instead potentiated AhR signaling, evident from increased CYP1A1 (cytochrome P450 family 1 subfamily A member 1), CYP1A2, and CYP1B1 expression. In pancreatic and liver cancer cells, Carbidopa promoted AhR nuclear localization. AhR antagonists blocked Carbidopa-dependent activation of AhR signaling. The inhibitory effect on pancreatic cancer cells in vitro and in vivo and the activation of AhR occurred at therapeutic concentrations of Carbidopa. Chromatin immunoprecipitation assay further confirmed that Carbidopa promoted AhR binding to its target gene CYP1A1 leading to its induction. We conclude that Carbidopa is an AhR agonist and suppresses pancreatic cancer. Hence, Carbidopa could potentially be re-purposed to treat pancreatic cancer and possibly other cancers as well.

scholarly journals Knockdown of lncRNA LINC01234 Suppresses The Tumorigenesis of Liver Cancer via Sponging miR-513a-5p

2020 ◽  
Author(s):  
Wen Xu ◽  
Kesang Li ◽  
Changfeng Song ◽  
Xiaotong Wang ◽  
Yueqi Li ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Bioinformatics Analysis ◽  
Tumor Model ◽  
Downstream Target ◽  
Normal Tissues ◽  
Liver Cancer Cells ◽  
Gene And Protein Expression ◽  
Cancer Tissues

Abstract Background: Liver cancer is a frequent malignancy with poor prognosis. It has been reported that many lncRNAs could regulate the progression of liver cancer. To identify potential therapeutic targets for liver cancer, we conducted bioinformatics analysis of lncRNAs in tumor tissues and adjacent normal tissues. Methods: The differential expression of lncRNAs between liver cancer tissues and adjacent normal tissues were examined by bioinformatics analysis. Cell proliferation was tested by CCK-8. Cell apoptosis in liver cancer was detected by flow cytometry. Gene and protein expression in liver cancer cells were measured by q-PCR and Western-blot, respectively. Xenograft tumor model was established to verify the function of LINC01234 on liver cancer in vivo.Results: LINC01234 was found to be notably upregulated in liver cancer tissues. In addition, knockdown of LINC01234 significantly inhibited the proliferation, invasion and induced the apoptosis of liver cancer cells. Meanwhile, miR-513a-5p was a downstream target of LINC01234 and USP4 was a direct target of miR-513a-5p. Moreover, downregulation of LINC01234 inhibited the tumorigenesis of liver cancer via inactivating TGF-β signaling.Conclusion: Downregulation of LINC01234 could inhibit the progression of liver cancer. Thus, LINC01234 may serve as a potential novel target for treatment of liver cancer.

scholarly journals Somatostatin Octapeptide Inhibits Cell Invasion and Metastasis in Hepatocellular Carcinoma Through PEBP1

2018 ◽  
Vol 47 (6) ◽  
pp. 2340-2349 ◽  
Author(s):  
Chuan-Zhong Huang ◽  
Ai-Min Huang ◽  
Jing-Feng Liu ◽  
Bin Wang ◽  
Ke-Can Lin ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Treatment Group ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Pulmonary Metastasis ◽  
Cell Invasion ◽  
Invasion And Metastasis ◽  
Liver Cancer Cells ◽  
Octreotide Treatment

Background/Aims: Hepatocellular carcinoma (HCC) is a major threat to human health. The condition carries a high risk of death; 45% of new cases occur in China. Surgical resection is the first choice for treatment of HCC, but 30.9% of patients experience recurrence within 6 months after the operation. To improve patient survival, we must determine how to reduce the probability of recurrence and metastasis and elucidate the underlying mechanism of disease. We therefore studied the effect of somatostatin octapeptide (octreotide) on the invasion and metastasis of HCC. Methods: The migration and invasion cytological tests were used to detect the effect of octreotide on liver cancer cells (SK-Hep-1 and HepG2). PEBP1 RNAi was used to knockdown expression. Invasion and metastasis were measured with transwell migration and wound-healing assays. Western blotting was used to detect changes in levels of PEBP1 and invasion pathway proteins after octreotide treatment. The effect of octreotide was studied in vivo by establishing a pulmonary metastasis model using SK-Hep-1 cells in nude mice. In-vivo bioluminescence imaging and hematoxylin and eosin staining of lung tissue were used to verify the results. Results: Increasing concentrations of octreotide were progressively more effective in halting the invasion and metastasis of liver cancer cells. Octreotide may upregulate PEBP1, TIMP-2, and E-cadherin while downregulating MMP-2 and Twist to inhibit cell invasion and metastasis. And downregulation of PEBP1 would also change the expression of MMP-2, TIMP-2 and Twist. The in-vivo experiments showed no cancer cell metastasis in 4 of the 6 mice in the octreotide-treatment group, while all of the mice in the control group displayed pulmonary metastasis of human HCC cells. And the survival period of the mice in the octreotide-treatment group was significantly prolonged. Conclusions: Octreotide may weaken invasion and metastasis through the upregulation of PEBP1. Octreotide may reduce the risk of recurrence and metastasis after surgery for liver cancer.

scholarly journals Ascorbic Acid Inhibits Liver Cancer Growth and Metastasis in vitro and in vivo, Independent of Stemness Gene Regulation

2021 ◽  
Vol 12 ◽  
Author(s):  
Jingjing Wan ◽  
Juan Zhou ◽  
Lu Fu ◽  
Yubin Li ◽  
Huawu Zeng ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Gene Regulation ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Cancer Metastasis ◽  
Liver Cancer Cells ◽  
Induced Apoptosis ◽  
Xenotransplantation Model

Experimental and clinical evidence has indicated that the natural product ascorbic acid (AA) is effective in preventing and treating various types of cancers. However, the effect of AA on liver cancer metastasis has not yet been reported. Cancer stem cells (CSCs) play pivotal roles in cancer metastasis. Here, we demonstrated that AA selectively inhibited the viability of both liver cancer cells and CSCs, reduced the formation of cancer cell colonies and CSC spheres, and inhibited tumor growth in vivo. Additionally, AA prevented liver cancer metastasis in a xenotransplantation model without suppressing stemness gene expression in liver CSCs. Further study indicated that AA increased the concentration of H2O2 and induced apoptosis in liver CSCs. Catalase attenuated the inhibitory effects of AA on liver CSC viability. In conclusion, AA inhibited the viability of liver CSCs and the growth and metastasis of liver cancer cells in vitro and in vivo by increasing the production of H2O2 and inducing apoptosis. Our findings provide evidence that AA exerts its anti-liver cancer efficacy in vitro and in vivo, in a manner that is independent of stemness gene regulation.

Sign in / Sign up

Export Citation Format

Share Document