Bromphenol Blue

Sodium Lauryl Sulfate in Egg Whites

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/50.4.847 ◽

1967 ◽

Vol 50 (4) ◽

pp. 847-849

Author(s):

John Wiskerchen

Keyword(s):

Chloride Solution ◽

Sodium Lauryl Sulfate ◽

Collaborative Study ◽

Egg White ◽

Chloride Complex ◽

Bromphenol Blue ◽

Lauryl Sulfate ◽

Benzethonium Chloride ◽

Alkyl Sulfates

Abstract A method is given for the quantitative determination of sodium lauryl sulfate in liquid, frozen, powdered, or flake-dried egg white. The egg white is dissolved in water and the protein is precipitated with ethanol and filtered off. The filtrate is evaporated, the residue is dissolved in water, and the pH is adjusted to 5.0. Total alkyl sulfates are titrated with standard benzethonium chloride solution in the presence of chloroform with bromphenol blue indicator. Results are calculated as sodium lauryl sulfate. The formation of the bromphenol bluebenzethonium chloride complex, when excess benzethonium chloride is present, is taken as the end point. The blue-green complex is soluble in the chloroform. Overall recoveries of sodium lauryl sulfate from egg whites ranged from 94 to 100%. Collaborative study of the method is recommended.

Dichromatism of Bromphenol Blue, with an Improvement in the Mercuric Bromphenol Blue Technic for Protein

Stain Technology ◽

10.3109/10520297509117027 ◽

1975 ◽

Vol 50 (1) ◽

pp. 25-30 ◽

Cited By ~ 16

Author(s):

D. M. Chapman

Keyword(s):

Bromphenol Blue

Use of Bromphenol Blue Printing Method for Detecting Sweat on the Palm

Journal of Hand Surgery (European Volume) ◽

10.1016/0266-7681_86_90034-3 ◽

1986 ◽

Vol 11 (1) ◽

pp. 125-130

Author(s):

M. SAKURAI

Keyword(s):

Sensory Nerve ◽

Sweat Glands ◽

Bromphenol Blue ◽

Nerve Function ◽

Nerve Fibres ◽

Recovery Stage ◽

Sympathetic Nerve Fibres ◽

The Many ◽

The One ◽

Printing Method

Sympathetic nerve fibres innervating the sweat glands in the skin are known to accompany sensory nerve fibres closely. Examination of sudorific function, therefore, is a useful aid in making a diagnosis of severed peripheral nerve and also provides valuable information on nerve function in the recovery stage following injury and surgery such as neurorrhaphy. Among the many methods which have been used clinically, the one employing bromphenol blue is thought to be the most simple and accurate.

Some of the Variables Involved in the Fractionation of Serum Proteins by Paper Electrophoresis

Clinical Chemistry ◽

10.1093/clinchem/3.1.49 ◽

1957 ◽

Vol 3 (1) ◽

pp. 49-64 ◽

Cited By ~ 30

Author(s):

Richard J Henry ◽

Orville J Golub ◽

Charles Sobel

Keyword(s):

Serum Proteins ◽

Normal Values ◽

Paper Electrophoresis ◽

Bromphenol Blue

Abstract Investigation of various steps involved in separation of serum proteins by paper electrophoresis and their quantitation by dyeing with bromphenol blue has revealed that the results obtained are quite dependent on a number of variables. It is essential, therefore, that rigid control of these variables be exercised and that normal values be obtained by the specific technic adopted. Normal values have been given for the technic employed and data on stability of sera at room and refrigerator temperatures have been presented.

Dependence of high serum aldolase on local circulation of injured muscle

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1962.202.6.1059 ◽

1962 ◽

Vol 202 (6) ◽

pp. 1059-1064 ◽

Cited By ~ 3

Author(s):

Amara Kitiyakara ◽

Imants Murmanis

Keyword(s):

General Circulation ◽

High Serum ◽

Bromphenol Blue ◽

Local Circulation ◽

Contralateral Limb ◽

Secondary Damage ◽

Prolonged Retention ◽

Aldolase Activity ◽

Temporary Decrease ◽

The Relationship

Studies were made on the relationship between local circulatory conditions of skeletal muscles subjected to a tourniquet ischemia and serum aldolase level. It was shown that during ischemia there was a rapid loss of aldolase activity of the injured muscles without a corresponding rise of the enzyme in the serum. Removal of the tourniquet after 4 hr of ischemia was followed by a partial resumption of local circulation evidenced by the staining of the damaged muscles by bromphenol blue introduced into the general circulation. A rapid rise of serum aldolase and further loss of aldolase activity of the injured muscles were closely associated with the partial re-establishment of the local circulation. In addition, uninjured muscles of the contralateral limb underwent a secondary damage demonstrated by a temporary decrease of aldolase activity and a prolonged retention of the dye. If the ischemia was maintained for 24 hr, removal of the tourniquet failed to re-establish the local circulation, and, consequently, there was no rise in serum aldolase nor secondary damage to the uninjured muscles.

An enzymic creatinine assay and a direct ammonia assay in coated thin films.

Clinical Chemistry ◽

10.1093/clinchem/29.4.645 ◽

1983 ◽

Vol 29 (4) ◽

pp. 645-649 ◽

Cited By ~ 13

Author(s):

M W Sundberg ◽

R W Becker ◽

T W Esders ◽

J Figueras ◽

C T Goodhue

Keyword(s):

Thin Film ◽

Thin Films ◽

Linear Regression ◽

Accurate Determination ◽

Bromphenol Blue ◽

Keto Acids ◽

Liquid Chromatographic ◽

Enzymic Assay ◽

Paired Analysis

Abstract We developed a thin-film enzymic assay for creatinine that makes use of creatinine iminohydrolase (EC 3.5.4.21) to convert creatinine to N-methylhydantoin and ammonia. The ammonia diffuses through a semipermeable layer and is quantitated by reaction with bromphenol blue. A paired analysis of the sample on a separate coating without the enzymic reaction measures endogenous ammonia and, for samples with normal concentrations of ammonia, allows accurate determination of serum creatinine to 150 mg/L without dilution. Results of this assay (y) compare well with those by a liquid-chromatographic comparison assay (x) by linear regression (slope = 0.935, intercept = 1.13 mg/L, r2 = 0.995). It is insensitive to many substances, such as ketones and keto acids, that interfere with conventional assays. Results of the ammonia assay (y) correlate well with those by a semi-automated enzymic assay (x) based on glutamate dehydrogenase (slope = 1.068, intercept = 17.3 mumol/L, r2 = 0.985).

ACS Reagent Chemicals ◽

10.1021/acsreagents.4063.20160601 ◽

2017 ◽

Keyword(s):

Bromphenol Blue

Quantitative Electrophoresis of Serum Proteins on Paper

Clinical Chemistry ◽

10.1093/clinchem/2.5.303 ◽

1956 ◽

Vol 2 (5) ◽

pp. 303-319 ◽

Cited By ~ 12

Author(s):

Moses Wurm ◽

Frederick H Epstein

Keyword(s):

Protein Concentration ◽

Moving Boundary ◽

Serum Proteins ◽

Paper Electrophoresis ◽

Bromphenol Blue ◽

Good Resolution ◽

Confidence Limits ◽

Protein Patterns ◽

Electrophoretic Patterns ◽

Normal Human

Abstract 1. A procedure for paper electrophoresis has been described which gives highly reproducible protein patterns with good resolution and freedom from distortions. 2. Densitometry of protein bands on paper stained with bromphenol blue or Amidoschwarz 10B reveals that the logarithm of protein concentration is proportional to optical density and that Beer's law does not apply. Electrophoretic patterns of normal human serum evaluated in this manner give values in close agreement with those obtained by moving-boundary electrophoresis. 3. Confidence limits were determined for both methods.

Prolonged Retention of 77Br labelled bromphenol blue in tumor tissue

International Journal of Nuclear Medicine and Biology ◽

10.1016/0047-0740(84)90034-2 ◽

1984 ◽

Vol 11 (1) ◽

pp. 60-63 ◽

Cited By ~ 2

Author(s):

R. Senekowitsch ◽

S. Möllenstädt

Keyword(s):

Tumor Tissue ◽

Bromphenol Blue ◽

Prolonged Retention

THE USE OF BROMPHENOL BLUE IN THE ASSAY OF RHEOMACRODEX EFFECTS ON FLAP VIABILITY

Plastic & Reconstructive Surgery ◽

10.1097/00006534-197302000-00030 ◽

1973 ◽

Vol 51 (2) ◽

pp. 206

Author(s):

Dicran Goulian

Keyword(s):

Bromphenol Blue ◽

Flap Viability