Glycinebetaine Promotes Photosynthesis, Biomass Accumulation, and Lipid Production in Nannochloropsis gaditana under Nitrogen Deprivation

2021 ◽  
Author(s):  
Chunhui Zhang ◽  
Runzhi Li ◽  
Yue Feng ◽  
Litao Zhang ◽  
Chunchao Zhao ◽  
...  
Keyword(s):  
Lipid Production ◽  
Biomass Accumulation ◽  
Nitrogen Deprivation ◽  
Nannochloropsis Gaditana

scholarly journals Lipid Production in Nannochloropsis gaditana during Nitrogen Starvation

Biology ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 5 ◽  
Author(s):  
Jorijn H. Janssen ◽  
René H. Wijffels ◽  
Maria J. Barbosa
Keyword(s):  
Nitrogen Starvation ◽  
De Novo ◽  
Lipid Production ◽  
Omega 3 ◽  
Supply Rate ◽  
Nannochloropsis Gaditana ◽  
Omega 3 Fatty Acid ◽  
Light Regimes ◽  
Lipid Fractions ◽  
Tag Accumulation

The microalga Nannochloropsis gaditana is a natural producer of triacylglycerol (TAG) and the omega-3 fatty acid eicosapentaenoic acid (EPA). TAG accumulation is induced by nitrogen starvation. The biomass-specific photon supply rate used had an effect on EPA and TAG accumulation during nitrogen starvation as well as on the localization of EPA accumulation. Clear differences in TAG yield on light were found for different biomass-specific photon supply rates and light regimes during nitrogen starvation. De novo EPA synthesis or the translocation of EPA between lipid fractions might be limiting for EPA accumulation in TAG. Further studies are needed to fully understand EPA accumulation in TAG during nitrogen starvation. To elucidate the function of EPA in TAG nitrogen recovery, experiments are suggested. The overexpression of genes involved in de novo EPA synthesis and translocation is proposed to elucidate the exact metabolic routes involved in these processes during nitrogen starvation. This work addresses future opportunities to increase EPA accumulation.

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator

2017 ◽  
Vol 35 (7) ◽  
pp. 647-652 ◽  
Author(s):  
Imad Ajjawi ◽  
John Verruto ◽  
Moena Aqui ◽  
Leah B Soriaga ◽  
Jennifer Coppersmith ◽  
...  
Keyword(s):  
Lipid Production ◽  
Transcriptional Regulator ◽  
Nannochloropsis Gaditana

scholarly journals A multi-omic characterization of temperature stress in a halotolerant Scenedesmus strain for algal biotechnology

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Sara Calhoun ◽  
Tisza Ann Szeremy Bell ◽  
Lukas R. Dahlin ◽  
Yuliya Kunde ◽  
Kurt LaButti ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Functional Genomics ◽  
Temperature Stress ◽  
Fatty Acid Biosynthesis ◽  
Lipid Production ◽  
Strain Improvement ◽  
Biomass Accumulation ◽  
Chemical Production ◽  
Algal Biotechnology ◽  
Response To Temperature

AbstractMicroalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus sp. NREL 46B-D3 demonstrating peak growth near 25 °C that reaches 30 g/m2/day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.

scholarly journals Dynamics of Photosynthesis in a Glycogen-DeficientglgCMutant of Synechococcus sp. Strain PCC 7002

2015 ◽  
Vol 81 (18) ◽  
pp. 6210-6222 ◽  
Author(s):  
Simon A. Jackson ◽  
Julian J. Eaton-Rye ◽  
Donald A. Bryant ◽  
Matthew C. Posewitz ◽  
Fiona K. Davies
Keyword(s):  
Carbon Fixation ◽  
Light Harvesting ◽  
Biomass Accumulation ◽  
Photosynthetic Electron Transport ◽  
Growth Conditions ◽  
Nitrogen Deprivation ◽  
Wild Type ◽  
Efficient Energy Transfer ◽  
Content Type ◽  
Complex Relationships

ABSTRACTCyanobacterial glycogen-deficient mutants display impaired degradation of light-harvesting phycobilisomes under nitrogen-limiting growth conditions and secrete a suite of organic acids as a putative reductant-spilling mechanism. This genetic background, therefore, represents an important platform to better understand the complex relationships between light harvesting, photosynthetic electron transport, carbon fixation, and carbon/nitrogen metabolisms. In this study, we conducted a comprehensive analysis of the dynamics of photosynthesis as a function of reductant sink manipulation in a glycogen-deficientglgCmutant ofSynechococcussp. strain PCC 7002. TheglgCmutant showed increased susceptibility to photoinhibition during the initial phase of nitrogen deprivation. However, after extended periods of nitrogen deprivation,glgCmutant cells maintained higher levels of photosynthetic activity than the wild type, supporting continuous organic acid secretion in the absence of biomass accumulation. In contrast to the wild type, theglgCmutant maintained efficient energy transfer from phycobilisomes to photosystem II (PSII) reaction centers, had an elevated PSII/PSI ratio as a result of reduced PSII degradation, and retained a nitrogen-replete-type ultrastructure, including an extensive thylakoid membrane network, after prolonged nitrogen deprivation. Together, these results suggest that multiple global signals for nitrogen deprivation are not activated in theglgCmutant, allowing the maintenance of active photosynthetic complexes under conditions where photosynthesis would normally be abolished.

scholarly journals Proteomic and Transcriptomic Patterns during Lipid Remodeling in Nannochloropsis gaditana

2020 ◽  
Vol 21 (18) ◽  
pp. 6946
Author(s):  
Chris J. Hulatt ◽  
Irina Smolina ◽  
Adam Dowle ◽  
Martina Kopp ◽  
Ghana K. Vasanth ◽  
...  
Keyword(s):  
Lipid Body ◽  
Gene Transcript ◽  
Nitrogen Deprivation ◽  
Oil Accumulation ◽  
Nannochloropsis Gaditana ◽  
Marine Microalga ◽  
Global Correlation ◽  
Tandem Mass Tag ◽  
Phytoplankton Communities ◽  
Associated Proteins

Nutrient limited conditions are common in natural phytoplankton communities and are often used to increase the yield of lipids from industrial microalgae cultivations. Here we studied the effects of bioavailable nitrogen (N) and phosphorus (P) deprivation on the proteome and transcriptome of the oleaginous marine microalga Nannochloropsis gaditana. Turbidostat cultures were used to selectively apply either N or P deprivation, controlling for variables including the light intensity. Global (cell-wide) changes in the proteome were measured using Tandem Mass Tag (TMT) and LC-MS/MS, whilst gene transcript expression of the same samples was quantified by Illumina RNA-sequencing. We detected 3423 proteins, where 1543 and 113 proteins showed significant changes in abundance in N and P treatments, respectively. The analysis includes the global correlation between proteomic and transcriptomic data, the regulation of subcellular proteomes in different compartments, gene/protein functional groups, and metabolic pathways. The results show that triacylglycerol (TAG) accumulation under nitrogen deprivation was associated with substantial downregulation of protein synthesis and photosynthetic activity. Oil accumulation was also accompanied by a diverse set of responses including the upregulation of diacylglycerol acyltransferase (DGAT), lipase, and lipid body associated proteins. Deprivation of phosphorus had comparatively fewer, weaker effects, some of which were linked to the remodeling of respiratory metabolism.

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