No Enhancement of Nuclear Entry by Direct Conjugation of a Nuclear Localization Signal Peptide to Linearized DNA

2003 ◽  
Vol 14 (6) ◽  
pp. 1197-1202 ◽  
Author(s):  
Mitsuhide Tanimoto ◽  
Hiroyuki Kamiya ◽  
Noriaki Minakawa ◽  
Akira Matsuda ◽  
Hideyoshi Harashima
Keyword(s):  
Nuclear Localization ◽  
Signal Peptide ◽  
Nuclear Localization Signal ◽  
Nuclear Entry ◽  
Localization Signal

Gene Delivery by PAMAM Dendrimer Conjugated with the Nuclear Localization Signal Peptide Derived from Influenza B Virus Nucleoprotein

2019 ◽  
Vol 27 (4) ◽  
pp. 360-368 ◽  
Author(s):  
Jeil Lee ◽  
Seulgi Lee ◽  
Yong-Eun Kwon ◽  
Youn-Joong Kim ◽  
Joon Sig Choi
Keyword(s):  
Gene Delivery ◽  
Nuclear Localization ◽  
Signal Peptide ◽  
Nuclear Localization Signal ◽  
Pamam Dendrimer ◽  
Influenza B Virus ◽  
Influenza B ◽  
B Virus ◽  
Localization Signal

One pot synthesis of highly luminescent polyethylene glycol anchored carbon dots functionalized with a nuclear localization signal peptide for cell nucleus imaging

Nanoscale ◽  
2015 ◽  
Vol 7 (14) ◽  
pp. 6104-6113 ◽  
Author(s):  
Lei Yang ◽  
Weihua Jiang ◽  
Lipeng Qiu ◽  
Xuewei Jiang ◽  
Daiying Zuo ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Nuclear Localization ◽  
Signal Peptide ◽  
Cell Nucleus ◽  
Nuclear Localization Signal ◽  
Carbon Dots ◽  
One Pot ◽  
One Pot Synthesis ◽  
Localization Signal

scholarly journals Gene delivery: A single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus

1999 ◽  
Vol 96 (1) ◽  
pp. 91-96 ◽  
Author(s):  
M. A. Zanta ◽  
P. Belguise-Valladier ◽  
J.-P. Behr
Keyword(s):  
Gene Delivery ◽  
Nuclear Localization ◽  
Signal Peptide ◽  
Cell Nucleus ◽  
Nuclear Localization Signal ◽  
Localization Signal

Priming of immune responses to hepatitis B surface antigen with minimal DNA expression constructs modified with a nuclear localization signal peptide

2001 ◽  
Vol 79 (5-6) ◽  
pp. 343-350 ◽  
Author(s):  
Reinhold Schirmbeck ◽  
Sven A. König-Merediz ◽  
Petra Riedl ◽  
Marcin Kwissa ◽  
Florian Sack ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Immune Responses ◽  
Nuclear Localization ◽  
Surface Antigen ◽  
Signal Peptide ◽  
Nuclear Localization Signal ◽  
Hepatitis B Surface Antigen ◽  
Localization Signal

scholarly journals The C-Terminal Region of PTHrP, in Addition to the Nuclear Localization Signal, Is Essential for the Intracrine Stimulation of Proliferation in Vascular Smooth Muscle Cells

Endocrinology ◽  
2001 ◽  
Vol 142 (9) ◽  
pp. 4096-4105 ◽  
Author(s):  
F. de Miguel ◽  
N. Fiaschi-Taesch ◽  
J. C. López-Talavera ◽  
K. K. Takane ◽  
T. Massfelder ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Muscle Cells ◽  
Nuclear Entry ◽  
C Terminus ◽  
Localization Signal

Abstract PTHrP is secreted by most cell types. In addition to a paracrine/autocrine role, PTHrP has “intracrine” actions, entering the nuclear compartment under the direction of a classic bipartite nuclear localization signal. In vascular smooth muscle cells, nuclear entry stimulates mitogenesis. In the current study, we sought to more precisely define the regions of PTHrP required for the activation of mitogenesis in vascular smooth muscle cells. PTHrP deletion mutants missing large regions [i.e. the signal peptide, N terminus (1–36), mid region (38–86), nuclear localization signal, C terminus (108–139), or combinations of the above] were expressed in A-10 vascular smooth muscle cells. The consequences on nuclear localization and proliferation were examined. Deletion of the nuclear localization signal prevented nuclear entry and slowed proliferation. Deletion of the highly conserved N terminus or mid region had no impact on nuclear localization or on proliferation. Deletion of the C terminus had no deleterious effect on nuclear localization but dramatically reduced proliferation. Thus, the nuclear localization signal is both necessary and sufficient for nuclear localization of PTHrP. In contrast, activation of proliferation in vascular smooth muscle cells requires both an intact nuclear localization signal and an intact C terminus. Whereas the nuclear localization signal is required for nuclear entry, the C terminus may serve a trans-activating function to stimulate mitogenesis once inside the nucleus of vascular smooth muscle cells.

Transcription Inhibition of OncogenicKRASby a Mutation-Selective Peptide Nucleic Acid Conjugated to the PKKKRKV Nuclear Localization Signal Peptide†

Biochemistry ◽  
2005 ◽  
Vol 44 (31) ◽  
pp. 10510-10519 ◽  
Author(s):  
S. Cogoi ◽  
A. Codognotto ◽  
V. Rapozzi ◽  
N. Meeuwenoord ◽  
G. van der Marel ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nuclear Localization ◽  
Signal Peptide ◽  
Nuclear Localization Signal ◽  
Peptide Nucleic Acid ◽  
Transcription Inhibition ◽  
Localization Signal
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