Multiple protein-binding domains and functional cis-elements in the 5'-flanking region of the human pyruvate dehydrogenase .alpha.-subunit gene

Biochemistry ◽  
1993 ◽  
Vol 32 (16) ◽  
pp. 4263-4269 ◽  
Author(s):  
Mei Chang ◽  
Sharon Naik ◽  
Gary L. Johanning ◽  
Lap Ho ◽  
Mulchand S. Patel
Keyword(s):  
Protein Binding ◽  
Pyruvate Dehydrogenase ◽  
Alpha Subunit ◽  
Cis Elements ◽  
Subunit Gene ◽  
Binding Domains ◽  
Multiple Protein ◽  
Flanking Region

scholarly journals A 5'-flanking region of the chicken acetylcholine receptor alpha-subunit gene confers tissue specificity and developmental control of expression in transfected cells.

1987 ◽  
Vol 7 (2) ◽  
pp. 951-955 ◽  
Author(s):  
A Klarsfeld ◽  
P Daubas ◽  
B Bourachot ◽  
J P Changeux
Keyword(s):  
Acetylcholine Receptor ◽  
Chloramphenicol Acetyltransferase ◽  
Alpha Subunit ◽  
Upstream Sequence ◽  
Subunit Gene ◽  
Base Pairs ◽  
Chicken Muscle ◽  
Flanking Region ◽  
Chloramphenicol Acetyltransferase Gene ◽  
Control Of Expression

The 5' end and promoter region of the alpha-subunit gene of chicken muscle acetylcholine receptor was mapped and sequenced. It includes a TATA and a CAAT box and a potential Sp1-binding site. When inserted in front of the chloramphenicol acetyltransferase gene, this promoter (including 850 base pairs of upstream sequence) directed high transient chloramphenicol acetyltransferase expression in transfected mouse C2.7 myotubes but not in C2.7 myoblasts or nonmyogenic 3T6 cells.

A 5'-flanking region of the chicken acetylcholine receptor alpha-subunit gene confers tissue specificity and developmental control of expression in transfected cells

1987 ◽  
Vol 7 (2) ◽  
pp. 951-955
Author(s):  
A Klarsfeld ◽  
P Daubas ◽  
B Bourachot ◽  
J P Changeux
Keyword(s):  
Acetylcholine Receptor ◽  
Chloramphenicol Acetyltransferase ◽  
Alpha Subunit ◽  
Upstream Sequence ◽  
Subunit Gene ◽  
Base Pairs ◽  
Chicken Muscle ◽  
Flanking Region ◽  
Chloramphenicol Acetyltransferase Gene ◽  
Control Of Expression

The 5' end and promoter region of the alpha-subunit gene of chicken muscle acetylcholine receptor was mapped and sequenced. It includes a TATA and a CAAT box and a potential Sp1-binding site. When inserted in front of the chloramphenicol acetyltransferase gene, this promoter (including 850 base pairs of upstream sequence) directed high transient chloramphenicol acetyltransferase expression in transfected mouse C2.7 myotubes but not in C2.7 myoblasts or nonmyogenic 3T6 cells.

scholarly journals The gonadotropin alpha-gene contains multiple protein binding domains that interact to modulate basal and cAMP-responsive transcription.

1988 ◽  
Vol 263 (20) ◽  
pp. 9879-9886
Author(s):  
J L Jameson ◽  
R C Jaffe ◽  
P J Deutsch ◽  
C Albanese ◽  
J F Habener
Keyword(s):  
Protein Binding ◽  
Binding Domains ◽  
Multiple Protein ◽  
Alpha Gene

Transient Prealbumin- Associated Hyperthyroxinemia in TSH-Producing Pituitary Adenoma

1990 ◽  
Vol 29 (01) ◽  
pp. 40-43 ◽  
Author(s):  
W. Langsteger ◽  
P. Költringer ◽  
P. Wakonig ◽  
B. Eber ◽  
M. Mokry ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Case Report ◽  
Pituitary Adenoma ◽  
Thyroid Hormones ◽  
Protein Binding ◽  
Alpha Subunit ◽  
Normal Range ◽  
Thyroxine Binding Globulin ◽  
Free Thyroid Hormones ◽  
Transmission Computed Tomography

This case report describes a 38-year-old male who was hospitalized for further clarification of clinically mild hyperthyroidism. His increased total hormone levels, the elevated free thyroid hormones and the elevated basal TSH with blunted response to TRH strongly suggested a pituitary adenoma with inappropriate TSH incretion. Transmission computed tomography showed an intrasellar expansion, 16 mm in diameter. The neoplastic TSH production was confirmed by an elevated alpha-subunit and a raised molar alpha-sub/ATSH ratio. However, T4 distribution on prealbumin (PA, TTR), albumin (A) and thyroxine binding globulin (TBG) showed a clearly increased binding to PA (39%), indicating additional prealbumin-associated hyperthyroxinemia. The absolute values of PA, A and TBG were within the normal range. After removal of the TSH-producing adenoma, basal TSH, the free thyroid hormones and T4 binding to prealbumin returned to normal. Therefore, the prealbumin-associated hyperthyroxinemia had to be interpreted as a transitory phenomenon related to secondary hyperthyroidism (T4 shift from thyroxine binding globulin to prealbumin) rather than a genetically conditioned anomaly of protein binding.

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