Molecular Reaction Mechanisms of Proteins Monitored by Time-Resolved FTIR and FT-Raman Spectroscopy

The near-infrared Fourier transform (NIR FT) Raman technique permits the measurement of Raman spectra without interference by fluorescence. Absorption by molecules containing X-H bonds in the NIR range requires a 180° scattering geometry. In this way, Raman spectroscopy of samples on surfaces is possible, both the detecting of small spots and the mapping of the sample distribution over larger areas. The spatial resolution extends into the micrometer range. Mapping of the inorganic pigment distribution of an initial letter of a mediaeval manuscript is demonstrated. For time-resolved measurements, the step-scan technique, previously developed for infrared spectroscopy, may be used in NIR FT-Raman spectroscopy as well. It allows the study of photochemical and photophysical processes, the application of modulation techniques, and the investigation of “noisy” samples. Photo-isomerization of the dye merocyanine 540 has been observed with the step-scan technique upon periodic excitation with a flash lamp.

Download Full-text

On-Line Monitoring of Airborne Chemistry in Levitated Nanodroplets: In Situ Synthesis and Application of SERS-Active Ag−Sols for Trace Analysis by FT-Raman Spectroscopy

Analytical Chemistry ◽

10.1021/ac026308l ◽

2003 ◽

Vol 75 (9) ◽

pp. 2166-2171 ◽

Cited By ~ 48

Author(s):

Nicolae Leopold ◽

Michael Haberkorn ◽

Thomas Laurell ◽

Johan Nilsson ◽

Josefa R. Baena ◽

...

Keyword(s):

Raman Spectroscopy ◽

Trace Analysis ◽

In Situ Synthesis ◽

Ft Raman Spectroscopy ◽

On Line ◽

Ft Raman

Download Full-text

Application of FT-Raman spectroscopy to the study of selected organometallic complexes and proteins

Canadian Journal of Chemistry ◽

10.1139/v90-185 ◽

1990 ◽

Vol 68 (7) ◽

pp. 1196-1200 ◽

Cited By ~ 7

Author(s):

Steven M. Barnett ◽

François Dicaire ◽

Ashraf A. Ismail

Keyword(s):

Raman Spectroscopy ◽

Near Infrared ◽

Metal Carbonyl ◽

Protein A ◽

Organometallic Complexes ◽

Laser Source ◽

Chromium Tricarbonyl ◽

Ft Raman Spectroscopy ◽

Arene Chromium Tricarbonyl ◽

Ft Raman

The study of colored organometallic complexes by dispersive Raman spectroscopy has been limited due to fluorescence or photodecomposition caused by the visible laser used as the excitation source. As a solution to this problem, FT-Raman spectroscopy with a near-infrared laser source has been useful in lowering fluorescence or photolysis in these samples. To investigate the utility of this technique, we have obtained and assigned the FT-Raman spectra of a series of arene chromium tricarbonyl complexes and of cyclopentadienyl manganese tricarbonyl. Some bands previously unobserved by dispersive Raman spectroscopy were seen, including a band assigned to a 13CO satellite in the spectrum of methylbenzoate chromium tricarbonyl. In addition, FT-Raman data for bovine serum albumin (BSA) and Protein-A are presented. Keywords: FT-Raman spectroscopy, metal carbonyl, proteins, organometallics, near infrared.

Download Full-text