New Sludge Pretreatment Method to Improve Methane Production in Waste Activated Sludge Digestion

2010 ◽  
Vol 44 (12) ◽  
pp. 4802-4808 ◽  
Author(s):  
Dong Zhang ◽  
Yinguang Chen ◽  
Yuxiao Zhao ◽  
Xiaoyu Zhu
Keyword(s):  
Activated Sludge ◽  
Methane Production ◽  
Waste Activated Sludge ◽  
Pretreatment Method ◽  
Sludge Digestion ◽  
Sludge Pretreatment

scholarly journals Genome-Centric Metagenomic Insights into the Impact of Alkaline/Acid and Thermal Sludge Pretreatment on the Microbiome in Digestion Sludge

2020 ◽  
Vol 86 (23) ◽  
Author(s):  
Zhiwei Liang ◽  
Jiangjian Shi ◽  
Chen Wang ◽  
Junhui Li ◽  
Dawei Liang ◽  
...  
Keyword(s):  
Organic Matter ◽  
Activated Sludge ◽  
Metabolic Networks ◽  
Waste Activated Sludge ◽  
Metabolic Potential ◽  
Sludge Digestion ◽  
Sludge Pretreatment ◽  
A Genome ◽  
Unique Source ◽  
The Impact

ABSTRACT Pretreatment of waste-activated sludge (WAS) is an effective way to destabilize sludge floc structure and release organic matter for improving sludge digestion efficiency. Nonetheless, information on the impact of WAS pretreatment on digestion sludge microbiomes, as well as mechanistic insights into how sludge pretreatment improves digestion performance, remains elusive. In this study, a genome-centric metagenomic approach was employed to investigate the digestion sludge microbiome in four sludge digesters with different types of feeding sludge: WAS pretreated with 0.25 mol/liter alkaline/acid (APAD), WAS pretreated with 0.8 mol/liter alkaline/acid (HS-APAD), thermally pretreated WAS (thermal-AD), and fresh WAS (control-AD). We retrieved 254 metagenome-assembled genomes (MAGs) to identify the key functional populations involved in the methanogenic digestion process. These MAGs span 28 phyla, including 69 yet-to-be-cultivated lineages, and 30 novel lineages were characterized with metabolic potential associated with hydrolysis and fermentation. Interestingly, functional populations involving carbohydrate digestion were enriched in APAD and HS-APAD, while lineages related to protein and lipid fermentation were enriched in thermal-AD, corroborating the idea that different substrates are released from alkaline/acid and thermal pretreatments. Among the major functional populations (i.e., fermenters, syntrophic acetogens, and methanogens), significant correlations between genome sizes and abundance of the fermenters were observed, particularly in APAD and HS-APAD, which had improved digestion performance. IMPORTANCE Wastewater treatment generates large amounts of waste-activated sludge (WAS), which consists mainly of recalcitrant microbial cells and particulate organic matter. Though WAS pretreatment is an effective way to release sludge organic matter for subsequent digestion, detailed information on the impact of the sludge pretreatment on the digestion sludge microbiome remains scarce. Our study provides unprecedented genome-centric metagenomic insights into how WAS pretreatments change the digestion sludge microbiomes, as well as their metabolic networks. Moreover, digestion sludge microbiomes could be a unique source for exploring microbial dark matter. These results may inform future optimization of methanogenic sludge digestion and resource recovery.

scholarly journals Nitrogen in the Process of Waste Activated Sludge Anaerobic Digestion

2014 ◽  
Vol 40 (2) ◽  
pp. 123-136 ◽  
Author(s):  
Jan Suschka ◽  
Klaudiusz Grübel
Keyword(s):  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Biogas Production ◽  
Sewage Treatment ◽  
Anaerobic Treatment ◽  
Waste Activated Sludge ◽  
Sludge Digestion ◽  
Sludge Pretreatment ◽  
Negative Side Effects ◽  
Pre Treatment

Abstract Primary or secondary sewage sludge in medium and large WWTP are most often processed by anaerobic digestion, as a method of conditioning, sludge quantity minimization and biogas production. With the aim to achieve the best results of sludge processing several modifications of technologies were suggested, investigated and introduced in the full technical scale. Various sludge pretreatment technologies before anaerobic treatment have been widely investigated and partially introduced. Obviously, there are always some limitations and some negative side effects. Selected aspects have been presented and discussed. The problem of nitrogen has been highlighted on the basis of the carried out investigations. The single and two step - mesophilic and thermophilic - anaerobic waste activated sludge digestion processes, preceded by preliminary hydrolysis were investigated. The aim of lab-scale experiments was pre-treatment of the sludge by means of low intensive alkaline and hydrodynamic disintegration. Depending on the pretreatment technologies and the digestion temperature large ammonia concentrations, up to 1800 mg NH4/dm3 have been measured. Return of the sludge liquor to the main sewage treatment line means additional nitrogen removal costs. Possible solutions are discussed.

New sludge pretreatment method to improve dewaterability of waste activated sludge

2011 ◽  
Vol 102 (10) ◽  
pp. 5659-5664 ◽  
Author(s):  
Hai-ping Yuan ◽  
Xiao-bo Cheng ◽  
Shan-ping Chen ◽  
Nan-wen Zhu ◽  
Zhen-ying Zhou
Keyword(s):  
Activated Sludge ◽  
Waste Activated Sludge ◽  
Pretreatment Method ◽  
Sludge Pretreatment

Enhancement of sludge decomposition and hydrogen production from waste activated sludge in a microbial electrolysis cell with cheap electrodes

2015 ◽  
Vol 1 (6) ◽  
pp. 761-768 ◽  
Author(s):  
Yinghong Feng ◽  
Yiwen Liu ◽  
Yaobin Zhang
Keyword(s):  
Hydrogen Production ◽  
Activated Sludge ◽  
Waste Activated Sludge ◽  
Electrolysis Cell ◽  
Microbial Electrolysis Cell ◽  
Graphite Electrodes ◽  
Sludge Digestion ◽  
Microbial Electrolysis

Cheap Fe/graphite electrodes substantially enhanced hydrogen production from anaerobic waste activated sludge digestion in a microbial electrolysis cell.

Effects of thermochemical pretreatment on the anaerobic digestion of waste activated sludge

1997 ◽  
Vol 35 (8) ◽  
pp. 209-215 ◽  
Author(s):  
Shuzo Tanaka ◽  
Toshio Kobayashi ◽  
Ken-ichi Kamiyama ◽  
Ma. Lolita N. Signey Bildan
Keyword(s):  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Production Rate ◽  
Methane Production ◽  
Waste Activated Sludge ◽  
Batch Experiments ◽  
Industrial Wastewaters ◽  
Thermochemical Pretreatment ◽  
Chemical Heating ◽  
Methane Production Rate

Effects of pretreatment on the anaerobic digestion of waste activated sludge (WAS) were investigated in terms of VSS solubilization and methane production by batch experiments. The methods of pretreatment studied are NaOH addition (chemical), heating (thermal) and heating with NaOH addition (thermochemical) to the domestic WAS and to the combined WAS from domestic, commercial and industrial wastewaters. The thermochemical pretreatment gave the best result among three methods in the combined WAS, i.e., the VSS was solubilized by 40-50% and the methane production increased by more than 200% over the control when the WAS was heated at 130°C for 5 minutes with the dose 0.3 g NaOH/g VSS. In the domestic WAS, the VSS solubilization rate was 70-80% but the increase of the methane production was about 30% after thermochemically pretreated. The domestic WAS consists of 41% protein, 25% lipid and 14% carbohydrate on COD basis, and the solubilization rate of protein, which is the largest constituent of the WAS, was 63% in the thermochemical pretreatment. Although the effect of the thermochemical pretreatment on the methane production was higher to the combined WAS than to the domestic WAS, the methane production rate was 21.9 ml CH4/g VSSWAS·day in the domestic WAS and 12.8 ml CH4/g VSSWAS·day in the combined WAS.

Influence of zero valent scrap iron (ZVSI) supply on methane production from waste activated sludge

2015 ◽  
Vol 263 ◽  
pp. 461-470 ◽  
Author(s):  
Guangyin Zhen ◽  
Xueqin Lu ◽  
Yu-You Li ◽  
Yuan Liu ◽  
Youcai Zhao
Keyword(s):  
Activated Sludge ◽  
Methane Production ◽  
Waste Activated Sludge ◽  
Scrap Iron

scholarly journals Thermophilic Alkaline Fermentation Followed by Mesophilic Anaerobic Digestion for Efficient Hydrogen and Methane Production from Waste-Activated Sludge: Dynamics of Bacterial Pathogens as Revealed by the Combination of Metagenomic and Quantitative PCR Analyses

2018 ◽  
Vol 84 (6) ◽  
Author(s):  
Jingjing Wan ◽  
Yuhang Jing ◽  
Yue Rao ◽  
Shicheng Zhang ◽  
Gang Luo
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Quantitative Pcr ◽  
Methane Production ◽  
Bacterial Pathogens ◽  
Methane Yield ◽  
Metagenomic Analysis ◽  
Waste Activated Sludge ◽  
Hydrogen Yield

ABSTRACT Thermophilic alkaline fermentation followed by mesophilic anaerobic digestion (TM) for hydrogen and methane production from waste-activated sludge (WAS) was investigated. The TM process was also compared to a process with mesophilic alkaline fermentation followed by a mesophilic anaerobic digestion (MM) and one-stage mesophilic anaerobic digestion (M) process. The results showed that both hydrogen yield (74.5 ml H 2 /g volatile solids [VS]) and methane yield (150.7 ml CH 4 /g VS) in the TM process were higher than those (6.7 ml H 2 /g VS and 127.8 ml CH 4 /g VS, respectively) in the MM process. The lowest methane yield (101.2 ml CH 4 /g VS) was obtained with the M process. Taxonomic results obtained from metagenomic analysis showed that different microbial community compositions were established in the hydrogen reactors of the TM and MM processes, which also significantly changed the microbial community compositions in the following methane reactors compared to that with the M process. The dynamics of bacterial pathogens were also evaluated. For the TM process, the reduced diversity and total abundance of bacterial pathogens in WAS were observed in the hydrogen reactor and were further reduced in the methane reactor, as revealed by metagenomic analysis. The results also showed not all bacterial pathogens were reduced in the reactors. For example, Collinsella aerofaciens was enriched in the hydrogen reactor, which was also confirmed by quantitative PCR (qPCR) analysis. The study further showed that qPCR was more sensitive for detecting bacterial pathogens than metagenomic analysis. Although there were some differences in the relative abundances of bacterial pathogens calculated by metagenomic and qPCR approaches, both approaches demonstrated that the TM process was more efficient for the removal of bacterial pathogens than the MM and M processes. IMPORTANCE This study developed an efficient process for bioenergy (H 2 and CH 4 ) production from WAS and elucidates the dynamics of bacterial pathogens in the process, which is important for the utilization and safe application of WAS. The study also made an attempt to combine metagenomic and qPCR analyses to reveal the dynamics of bacterial pathogens in anaerobic processes, which could overcome the limitations of each method and provide new insights regarding bacterial pathogens in environmental samples.

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