A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection

The nucleic acid-based technique has been widely utilized in many fields including for on-site detection. However, traditional molecular detection techniques encounter limitations like relying on instruments, time consuming or complex operation, and cannot meet the demands of on-site testing. In this study, a rapid DNA extraction method (RDEM), recombinase aided amplification (RAA), and chemical heating packet (CHP) are integrated and termed as RRC platform for on-site detection of nucleic acid. For demonstration purposes, SHZD32-1 (a new transgenic soybean line from China) was detected using the novel platform to demonstrate its feasibility and capability for on-site detection. Using the RDEM, high-quality DNA appropriate for molecular detection was quickly extracted in 3–5 min. The heat energy generated by CHP was met the temperature requirements of RAA. Using the RRC platform, the whole detection process can be accomplished within only 30 min, and the results can be visually detected with glasses under blue light. No special or expensive instrument was needed for the detection process. This study provides a novel approach for on-site detection of nucleic acids besides providing valuable insight on related future research.

Gravity wave instability structures and turbulence from more than 1.5 years of OH* airglow imager observations in Slovenia

We analysed 286 nights of data from the OH* airglow imager FAIM 3 (Fast Airglow IMager) acquired at Otlica Observatory (45.93∘ N, 13.91∘ E), Slovenia, between 26 October 2017 and 6 June 2019. Measurements have been performed with a spatial resolution of 24 m per pixel and a temporal resolution of 2.8 s. A two-dimensional fast Fourier transform is applied to the image data to derive horizontal wavelengths between 48 m and 4.5 km in the upper mesosphere/lower thermosphere (UMLT) region. In contrast to the statistics of larger-scale gravity waves (horizontal wavelength up to ca. 50 km; Hannawald et al., 2019), we find a more isotropic distribution of directions of propagation, pointing to the presence of wave structures created above the stratospheric wind fields. A weak seasonal tendency of a majority of waves propagating eastward during winter may be due to instability features from breaking secondary gravity waves that were created in the stratosphere. We also observe an increased southward propagation during summer, which we interpret as an enhanced contribution of secondary gravity waves created as a consequence of primary wave filtering by the meridional mesospheric circulation. We present multiple observations of turbulence episodes captured by our high-resolution airglow imager and estimated the energy dissipation rate in the UMLT from image sequences in 25 cases. Values range around 0.08 and 9.03 W kg−1 and are on average higher than those in recent literature. The values found here would lead to an approximated localized maximum heating of 0.03–3.02 K per turbulence event. These are in the same range as the daily chemical heating rates for the entire atmosphere reported by Marsh (2011), which apparently stresses the importance of dynamical energy conversion in the UMLT.

Gravity wave instability structures and turbulence from more than one and a half years of OH* airglow imager observations in Slovenia

We analysed 286 nights of data from the OH* airglow imager FAIM 3 (Fast Airglow IMager) acquired at Otlica Observatory (45.93 °N, 13.91 °E), Slovenia between 26 October 2017 and 6 June 2019. Measurements have been performed with a spatial resolution of 24 m/pixel and a temporal resolution of 2.8 s. A two-dimensional Fast Fourier transform is applied to the image data to derive horizontal wavelengths between 48 m and 4.5 km in the upper mesosphere / lower thermosphere (UMLT) region. In contrast to the statistics of larger scale gravity waves (horizontal wavelength up to ca. 50 km) we find a more isotropic distribution of directions of propagation, pointing to the presence of wave structures created above the stratospheric wind fields. A weak seasonal tendency of a majority of waves propagating eastward (westward) during winter (summer) may be due to secondary gravity waves originating from breaking primary waves in the stratosphere. We also observe an increased southward propagation during summer, which we interpret as an enhanced contribution of secondary gravity waves created as a consequence of primary wave filtering by the meridional mesospheric circulation. Furthermore, observations of turbulent vortices allowed the estimation of eddy diffusion coefficients in the UMLT from image sequences in 45 cases. Values range around 103–104 m2s-1 and mostly agree with literature. Turbulently dissipated energy is derived taking into account values of the Brunt-Väisälä frequency based on TIMED-SABER (Thermosphere Ionosphere Mesosphere Energetics Dynamics, Sounding of the Atmosphere using Broadband Emission Radiometry) measurements. Energy dissipation rates range between 0.63 W/kg and 14.21 W/kg leading to an approximated maximum heating of 0.2–6.3 K per turbulence event. These are in the same range as the daily chemical heating rates, which apparently stresses the importance of dynamical energy conversion in the UMLT.

Prediction of Probabilistic Shock Initiation Thresholds of Energetic Materials through Evolution of Thermal-mechanical Dissipation and Reactive Heating

The ignition threshold of an energetic material (EM) quantifies the macroscopic conditions for the onset of self-sustaining chemical reactions. The threshold is an important theoretical and practical measure of material attributes that relate to safety and reliability. Historically, the thresholds are measured experimentally. Here, we present a new Lagrangian computational framework for establishing the probabilistic ignition thresholds of heterogeneous EM out of the evolutions of coupled mechanical-thermal-chemical processes using mesoscale simulations. The simulations explicitly account for microstructural heterogeneities, constituent properties, and interfacial processes and capture processes responsible for the development of material damage and the formation of hotspots in which chemical reactions initiate. The specific mechanisms tracked include viscoelasticity, viscoplasticity, fracture, post-fracture contact, frictional heating, heat conduction, reactive chemical heating, gaseous product generation, and convective heat transfer. To determine the ignition threshold, the minimum macroscopic loading required to achieve self-sustaining chemical reactions with rate of reactive heat generation exceeding the rate of heat loss due to conduction and other dissipative mechanisms is determined. Probabilistic quantification of the processes and the thresholds are obtained via the use of statistically equivalent microstructure samples sets (SEMSS). The predictions are in agreement with available experimental data.

A review of domestic and foreign experience of steel treatment in CCM tundishes

The modern steelmaking facilities melt as a rule a semi product, which is further subjected to various processes of refining in steel ladle. Recently an additional treatment of the metal in CCM tundish became widespread in the domestic and foreign practice. A tendency of transforming tundish into a multifunctional metallurgical reactor was noted, since more and more technological operations are transferred in it, including alloying, stirring, various methods of heating, modifying and removal of nonmetallic inclusions. Examples of comprehensive utilization of a bloom CCM tundish at some Japanese plants of Kobe Steel in Kakagava presnted, at which the metal is filtered when going through holes in the two partitions thus effectively removing nonmetallic inclusions. New variants of metal blowing off in the tundish by inert gas developed by domestic specialists considered, including a technology for metal blowing off by an inert gas and a facility for the inert gas supply through the stopper of the tundish. Supply of inert gas through the stopper results in efficiency increasing of degassing and nonmetallic inclusions removal as well as submerged nuzzles service time increase, which is particularly important at casting of steels with high content of aluminum. Examples of solutions of metal treatment in tundish by cored and aluminum wire given. Schemes of cored wire introduction into tundish and liquid steel treatment in a CCM mold considered. Considerable attention was given to the problem of metal temperature control in tundish, including by an electric arc, induction and plasma heating. List of domestic and foreign plants presented, implemented facilities of steel plasma heating in the CCM tundish. It was noted, that steel chemical heating in tundish can be applied at unforeseen problems arising at casting.

Roto-Chemical Heating with Fall-Back Disk Accretion in the Neutron Stars Containing Quark Matter

Probing quark matter is one of the important tasks in the studies of neutron stars (NS). Some works explicitly consider the existence of quark matter in the appearance of hybrid star (HS) or pure quark star (QS). In the present work, we study the roto-chemical heating with accretion in HS and QS, and compare their chemical evolution and cooling features with pure NS. Different from HS and NS, there are two jumps in the chemical evolution of QS, which results from the fast direct Urca (Durca) reaction causing the fast recovery to chemical balance. However, the sudden change in the chemical evolution doesn’t provide an obvious heating effect in the thermal evolution. Differently, the roto-chemical heating effect appears both in the accretion phase and spin-down phase of the HS, and the heating platform in the accretion phase relies on the accretion rate. Larger accretion rate results in larger chemical deviation, higher and longer heating platform, and earlier appearance of the heating effect. Interestingly, with the disappearance of the heating effect in the accretion phase, the surface temperature drops fast, which is another possibility of the rapid cooling trend of the NS in Cas A. Furthermore, the surface temperature of the QS is obviously lower than the HS and NS, which is a latent candidate for the explanation of the old classical pulsar J2144-3933 with the lowest known surface temperature.

Radiation thermo-chemical models of protoplanetary disks

Context. Disks around pre-main-sequence stars evolve over time by turbulent viscous spreading. The main contender to explain the strength of the turbulence is the magnetorotational instability model, whose efficiency depends on the disk ionization fraction. Aims. Our aim is to compute self-consistently the chemistry including polycyclic aromatic hydrocarbon (PAH) charge chemistry, the grain charging, and an estimate of an effective value of the turbulence α parameter in order to find observational signatures of disk turbulence. Methods. We introduced PAH and grain charging physics and their interplay with other gas-phase reactions in the physico-chemical code PRODIMO. Non-ideal magnetohydrodynamics effects such as ohmic and ambipolar diffusion are parametrized to derive an effective value for the turbulent parameter αeff. We explored the effects of turbulence heating and line broadening on CO isotopologue submillimeter lines. Results. The spatial distribution of αeff depends on various unconstrained disk parameters such as the magnetic parameter βmag or the cosmic ray density distribution inside the protoplanetary disk s. The inner disk midplane shows the presence of the so-called dead zone where the turbulence is almost inexistent. The disk is heated mostly by thermal accommodation on dust grains in the dead zone, by viscous heating outside the dead zone up to a few hundred astronomical units, and by chemical heating in the outer disk. The CO rotational lines probe the warm molecular disk layers where the turbulence is at its maximum. However, the effect of turbulence on the CO line profiles is minimal and difficult to distinguish from the thermal broadening. Conclusions. Viscous heating of the gas in the disk midplane outside the dead zone is efficient. The determination of α from CO rotational line observations alone is challenging.

Multi-stage chemical heating for instrument-free biosensing

Improving the portability of diagnostic medicine is crucial to alleviating global access-to-care deficiencies. This requires not only designing devices that are small and lightweight but also autonomous and independent of electricity. Here, we present a strategy for conducting automated multi-step diagnostic assays using chemically generated, passively regulated heat. Ligation and polymerization reagents for Rolling Circle Amplification of nucleic acids are separated by melt-able phase-change partitions, thus replacing precise manual reagent additions with automated partition melting. To actuate these barriers and individually initiate the various steps of the reaction, field ration heaters exothermically generate heat in a thermos while fatty acids embedded in a carbonaceous matrix passively buffer the temperature around their melting points. Achieving multi-stage temperature profiles extends the capability of instrument-free diagnostic devices and improves the portability of reaction automation systems built around phase-change partitions.