Comparative Assessments of Benzene, Toluene, and Xylene Natural Attenuation by Quantitative Polymerase Chain Reaction Analysis of a Catabolic Gene, Signature Metabolites, and Compound-Specific Isotope Analysis

2008 ◽  
Vol 42 (16) ◽  
pp. 6065-6072 ◽  
Author(s):  
Harry R. Beller ◽  
Staci R. Kane ◽  
Tina C. Legler ◽  
Jennifer R. McKelvie ◽  
Barbara Sherwood Lollar ◽  
...  
Keyword(s):  
Natural Attenuation ◽  
Quantitative Polymerase Chain Reaction ◽  
Isotope Analysis ◽  
Polymerase Chain Reaction Analysis ◽  
Gene Signature ◽  
Catabolic Gene ◽  
Chain Reaction ◽  
Compound Specific Isotope Analysis ◽  
Benzene Toluene ◽  
Polymerase Chain

Expression of vascular endothelial growth factor A isoforms is dysregulated in women with endometriosis

2018 ◽  
Vol 30 (4) ◽  
pp. 651 ◽  
Author(s):  
Kevin Danastas ◽  
Emily J. Miller ◽  
Alison J. Hey-Cunningham ◽  
Christopher R. Murphy ◽  
Laura A. Lindsay
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Vascular Endothelial ◽  
Chain Reaction ◽  
Natural Production ◽  
Polymerase Chain ◽  
Endothelial Growth Factor ◽  
Natural Expression

Angiogenesis is a critical step in the development of ectopic lesions during endometriosis. Although total vascular endothelial growth factor (VEGF) A is elevated in the peritoneal fluid of women with endometriosis, there are contradictory reports on how levels of total endometrial VEGFA are altered in this disease. Furthermore, limited research is available on different VEGFA isoforms in women with endometriosis. Thus, the aim of the present study was to analyse levels of various VEGFA isoforms in women with and without endometriosis at different stages of the menstrual cycle. Quantitative polymerase chain reaction analysis showed that total VEGFA was highest during menstruation in endometriosis compared with controls (P = 0.0373). VEGF121 and VEGF189 were similarly highest during menstruation in endometriosis compared with controls (P = 0.0165 and 0.0154 respectively). The present study is also the first to identify the natural expression of VEGF111 in human tissue, which is also highest during menstruation in endometriosis (P = 0.0464). This discovery of the natural production of VEGF111 in human endometrium, as well as the upregulation of VEGFA isoforms during menstruation in endometriosis, may shed further light on the development and progression of the disease, and improve our understanding of the regulation of endometrial angiogenesis.

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