First Ultraperformance Liquid Chromatography Based Strategy for Profiling Intact Proteins in Complex Matrices: Application to the Evaluation of the Performance of Olive (Olea europaea L.) Stone Proteins for Cultivar Fingerprinting

2010 ◽  
Vol 58 (14) ◽  
pp. 8176-8182 ◽  
Author(s):  
Clara Esteve ◽  
Carmen Del Río ◽  
María Luisa Marina ◽  
María Concepción García
2013 ◽  
Vol 141 (4) ◽  
pp. 4375-4381 ◽  
Author(s):  
Marta Sánchez-González ◽  
Glòria Lozano-Mena ◽  
M. Emília Juan ◽  
Andrés García-Granados ◽  
Joana M. Planas

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2555 ◽  
Author(s):  
Eliana Alves ◽  
Tânia Melo ◽  
Madalena P. Barros ◽  
M. Rosário M. Domingues ◽  
Pedro Domingues

Olives (Olea europaea L.) are classic ingredients in the Mediterranean diet with well-known health benefits, but their lipid composition has not been fully addressed. In this work, we characterised triacylglycerol (TAG) and polar lipid profiles of the olive pulp while using a complementary methodological approach that was based on solid-phase extraction to recover the neutral lipid (NL) and the polar lipid-rich fractions. The TAG profile was analysed in the NL-fraction by C30 reversed-phase liquid chromatography (LC) and the polar lipid profile by normal-phase hydrophilic interaction liquid chromatography (HILIC), with both being coupled to electrospray ionization-mass spectrometry (ESI-MS) and ESI-MS/MS. This approach identified 71 TAG ions that were attributed to more than 350 molecular species, with fatty acyl chain lengths from C11:0 to C26:0, including different polyunsaturated acyl chains. The polar lipids included 107 molecular species that belonged to 11 lipid classes that comprised phospholipids, glyceroglycolipids, glycosphingolipids, and betaine lipids. In addition to polyunsaturated fatty acids, some of the phospholipids, glycolipids, and glycosphingolipids that were identified in the olive pulp have been described as biologically active molecules. Lipidomic phenotyping of the olive pulp has led to the discovery of compounds that will allow for a better assessment of its nutritional value and new applications of bioactive lipid components in this functional food.


1990 ◽  
Vol 16 (1) ◽  
pp. 113-121 ◽  
Author(s):  
Charles Baldy

Grana ◽  
2016 ◽  
Vol 56 (3) ◽  
pp. 204-214 ◽  
Author(s):  
Rita Messora ◽  
Assunta Florenzano ◽  
Paola Torri ◽  
Anna Maria Mercuri ◽  
Innocenzo Muzzalupo ◽  
...  

2019 ◽  
Vol 677 ◽  
pp. 350-361 ◽  
Author(s):  
M. Vilarrasa-Nogué ◽  
M.R. Teira-Esmatges ◽  
J.M. Villar ◽  
J. Rufat

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