Metabolomic Analysis of Phenolic Compounds in Buckwheat (Fagopyrum esculentum M.) Sprouts Treated with Methyl Jasmonate

Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of ‘Parthenon’ broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total flavonoids and antioxidant activity were measured to evaluate the overall quality. Moreover, individual carotenoids, phenolic compounds and glucosinolates were evaluated by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography equipped with diode array detector coupled to mass spectrometer using electro spray ionization (HPLC-DAD-ESI/MSn). The content of total carotenoids, phenolic compounds and glucosinolates were higher in autumn compared with spring, showing increases of 2.8-fold, 2-fold and 1.2-fold, respectively. Moreover, a double application of MeJA increased the contents of total carotenoids, phenolic compounds and glucosinolates by 22%, 32% and 39%, respectively, relative to the untreated samples. Considering our results, the controlled and timely application of 250 µM MeJA to the aerial parts of the plants four days before harvest, on two consecutive days, seems to be a valid agronomic strategy to improve the health-promoting capacity of Parthenon broccoli, without compromising its overall quality.

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Buckwheat (Fagopyrum esculentum M.) Sprout Treated with Methyl Jasmonate (MeJA) Improved Anti-Adipogenic Activity Associated with the Oxidative Stress System in 3T3-L1 Adipocytes

International Journal of Molecular Sciences ◽

10.3390/ijms14011428 ◽

2013 ◽

Vol 14 (1) ◽

pp. 1428-1442 ◽

Cited By ~ 13

Author(s):

Young-Jun Lee ◽

Kui-Jin Kim ◽

Kee-Jai Park ◽

Bo-Ra Yoon ◽

Jeong-Ho Lim ◽

...

Keyword(s):

Oxidative Stress ◽

Methyl Jasmonate ◽

Fagopyrum Esculentum ◽

Stress System

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Effect of Methyl Jasmonate on Phenolic Compounds and Carotenoids of Romaine Lettuce (Lactuca sativa L.)

Journal of Agricultural and Food Chemistry ◽

10.1021/jf071927m ◽

2007 ◽

Vol 55 (25) ◽

pp. 10366-10372 ◽

Cited By ~ 71

Author(s):

Hyun-Jin Kim ◽

Jorge M. Fonseca ◽

Ju-Hee Choi ◽

Chieri Kubota

Keyword(s):

Phenolic Compounds ◽

Methyl Jasmonate ◽

Lactuca Sativa ◽

Romaine Lettuce ◽

Lactuca Sativa L

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Increasing Bioactive Phenolic Compounds in Grapes: Response of Six Monastrell Grape Clones to Benzothiadiazole and Methyl Jasmonate Treatments

American Journal of Enology and Viticulture ◽

10.5344/ajev.2013.13038 ◽

2013 ◽

Vol 64 (4) ◽

pp. 459-465 ◽

Cited By ~ 20

Author(s):

Y. Ruiz-Garcia ◽

I. Romero-Cascales ◽

A. B. Bautista-Ortin ◽

R. Gil-Munoz ◽

A. Martinez-Cutillas ◽

...

Keyword(s):

Phenolic Compounds ◽

Methyl Jasmonate

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Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour

Journal of Ethnopharmacology ◽

10.1016/s0378-8741(00)00196-3 ◽

2000 ◽

Vol 72 (1-2) ◽

pp. 35-42 ◽

Cited By ~ 452

Author(s):

Christel Quettier-Deleu ◽

Bernard Gressier ◽

Jacques Vasseur ◽

Thierry Dine ◽

Claude Brunet ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Activities ◽

Fagopyrum Esculentum ◽

Fagopyrum Esculentum Moench

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Study on Phenolic Compounds and Effect of Time-Temperature Treatment on Amylase Activities of High Hill Buckwheat in Nepal

Journal of Food Science and Technology Nepal ◽

10.3126/jfstn.v8i0.11749 ◽

2014 ◽

Vol 8 ◽

pp. 46-51

Author(s):

Pradeep Kaji Poudel ◽

Dhan B. Karki

Keyword(s):

Phenolic Compounds ◽

Proximate Composition ◽

Total Phenolics ◽

Amylase Activity ◽

Temperature Treatment ◽

Fagopyrum Esculentum ◽

Total Flavonoids ◽

Germination Time ◽

Different Temperatures

Two varieties of buckwheat, mithe, Fagopyrum esculentum and tite, Fagopyurum tataricum,were collected from Mustang district, Nepal, and their proximate composition was analyzed. Total flavonoids, Tannin and Total phenolics were significantly higher (p<0.05) in tite than in mithe buckwheat. Samples of Buckwheat were steeped in water for 12 h and germinated for 48, 72, 96, 120, and 144 h at eachtem perature of 15, 20, 23 and 27 ºC. The germination inhibited by stepwise drying from 50 to 80 ºC. Effect of germination time and buckwheat variety on amylase activity was studied. Germination time had a significant effect on amylase activity of buckwheat malts. Tite and mithe buckwheat were grown under four different temperatures at 15, 20, 23 and 27 ºC for 48, 72,96, 120 and 144 h. However, mithe buckwheat was not germinated at 27 ºC. The maximum amylase activity was observed at 23ºC for 96 h. At this temperature mithe and tite buckwheat had alpha and beta amylase activity 13.87 and 268.97 units/gdry malt and 13.18 and 275.96 units/g dry malt,respectively. Due to high amylase activity of tite buckwheat was selected and malted in bulk quantity. DOI: http://dx.doi.org/10.3126/jfstn.v8i0.11749 J. Food Sci. Technol. Nepal, Vol. 8 (46-51), 2013

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The effect of methyl jasmonate vapors on content of phenolic compounds in seedlings of common buckwheat (Fagopyrum esculentum Moench)

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2011.001 ◽

2011 ◽

Vol 80 (1) ◽

pp. 5-9 ◽

Cited By ~ 12

Author(s):

Marcin Horbowicz ◽

Grzegorz Chrzanowski ◽

Danuta Koczkodaj ◽

Joanna Mitrus

Keyword(s):

Phenolic Compounds ◽

Methyl Jasmonate ◽

Chlorogenic Acid ◽

Phenolic Acids ◽

Total Phenolics ◽

Quercetin Glycosides ◽

Free Phenolic Acids ◽

Small Decline ◽

Fagopyrum Esculentum Moench ◽

Higher Doses

The effect of methyl jasmonate (MJ) vapors on content of phenolic compounds: free phenolic acids, total quercetin, and total phenolics in etiolated buckwheat seedlings were studied. The data presented show that low concentration of MJ (10-8 M) had no influence on trans-cinnamic acid (CA), but stimulated the accumulation of chlorogenic acid in hypocotyls and cotyledons of buckwheat seedlings. A moderate dose of MJ (10-6 M) did not change the level of chlorogenic acid in the hypocotyls and cotyledons, but CA synthesis was promoted in cotyledons, whereas in hypocotyls no significant effect was found. Highest concentration of MJ (10-4 M) caused small decline of CA in hypocotyls, but large stimulation of the acid production in cotyledons was noted. MJ had stimulatory effect on caffeic acid forming, but inhibited synthesis of vanillic acid in hypocotyls and cotyledons. Lowest concentration of MJ (10-8 M) elicited accumulation of quercetin glycosides in both studied tissues of buckwheat seedlings, however at higher doses (10-8 and 10-4 M) did not affect the flavonol level. The obtained results suggest that nonequivalent influence of methyl jasmonate on the phenolics composition can be a result of various mechanisms of MJ uptake, transforming and/or its translocation in buckwheat hypocotyls and cotyledons. Decline of anthocyanins level in buckwheat hypocotyls caused by MJ cannot be explained by enhanced accumulation of quercetin glycosides or free phenolic acids, but probably by synthesis of other unknown phenolic compounds.

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