Podophyllotoxin and Deoxypodophyllotoxin in Juniperus bermudiana and 12 OtherJuniperusSpecies: Optimization of Extraction, Method Validation, and Quantification

2011 ◽  
Vol 59 (15) ◽  
pp. 8101-8107 ◽  
Author(s):  
Sullivan Renouard ◽  
Tatiana Lopez ◽  
Oktavia Hendrawati ◽  
Patricia Dupre ◽  
Joël Doussot ◽  
...  
Keyword(s):  
Method Validation ◽  
Extraction Method ◽  
Validation And Quantification

Analysis of primaquine and its metabolite carboxyprimaquine in biological samples: enantiomeric separation, method validation and quantification

2010 ◽  
Vol 25 (9) ◽  
pp. 1010-1017 ◽  
Author(s):  
Bharathi Avula ◽  
Shabana I. Khan ◽  
Babu L. Tekwani ◽  
N.P. Dhammika Nanayakkara ◽  
James D. McChesney ◽  
...  
Keyword(s):  
Method Validation ◽  
Biological Samples ◽  
Enantiomeric Separation ◽  
Separation Method ◽  
Validation And Quantification

Pesticide residues analysis in honey using ethyl acetate extraction method: validation and pilot survey in real samples

2015 ◽  
Vol 95 (10) ◽  
pp. 894-910 ◽  
Author(s):  
Despo Louca Christodoulou ◽  
Popi Kanari ◽  
Olympiada Kourouzidou ◽  
Maria Constantinou ◽  
Panayiota Hadjiloizou ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Method Validation ◽  
Pesticide Residues ◽  
Extraction Method ◽  
Real Samples ◽  
Pilot Survey

Evaluation of the dark field method for large association of spread DNA

1978 ◽  
Vol 36 (2) ◽  
pp. 190-191
Author(s):  
Douglas C. Barker
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Mitochondrial Dna ◽  
Extraction Method ◽  
Field Method ◽  
Dark Field ◽  
Kinetoplast Dna ◽  
Field Electron ◽  
Field Electron Microscopy ◽  
Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

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