Correction to Indirect Competitive ELISA for Determination of Traces of Peanut (Arachis hypogaea L.) Protein in Complex Food Matrices

2012 ◽  
Vol 60 (45) ◽  
pp. 11446-11446
Author(s):  
Thomas Holzhauser ◽  
Stefan Vieths
Keyword(s):  
Arachis Hypogaea ◽  
Competitive Elisa ◽  
L Protein ◽  
Indirect Competitive Elisa ◽  
Arachis Hypogaea L ◽  
Food Matrices

scholarly journals Evaluation of Tertiary Butylhydroquinone as an Antioxidant in Powdered Roasted Peanuts Products1

1979 ◽  
Vol 6 (1) ◽  
pp. 55-57 ◽  
Author(s):  
M. W. Hoover ◽  
P. N. Painter
Keyword(s):  
Quantitative Determination ◽  
Shelf Life ◽  
Arachis Hypogaea ◽  
Drug Administration ◽  
Food And Drug Administration ◽  
Fat Content ◽  
Tertiary Butylhydroquinone ◽  
Life Study ◽  
Arachis Hypogaea L

Abstract A fifteen month shelf life study was conducted to determine the effectiveness of tertiary butylhydroquinone (TBHQ) as an antioxidant in products made from roasted peanuts (Arachis hypogaea L.). Five levels of TBHQ were studied, 0.0%, 0.01%, 0.02%, 0.03%, and 0.04% based on the fat content of the peanuts. Total carbonyl assays performed monthly served as indices of rancidity. Results indicate that the shelf life of the product may be extended up to thirteen months using the 0.02% level of TBHQ now allowed by the Food and Drug Administration. In addition, the quantitative determination of total carbonyls proved to be an acceptable indicator of rancidity of roasted peanuts.

Detection of total bile acids in biological samples using an indirect competitive ELISA based on four monoclonal antibodies

2017 ◽  
Vol 9 (4) ◽  
pp. 625-633 ◽  
Author(s):  
Shuchen Liu ◽  
Yue Zhang ◽  
Baoping Qu ◽  
Gaofeng Qin ◽  
Jinjun Cheng ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Clinical Practice ◽  
Biological Samples ◽  
Competitive Elisa ◽  
Routine Clinical Practice ◽  
Enzyme Linked Immunosorbent Assay ◽  
Indirect Competitive Elisa ◽  
Different Types ◽  
Total Bile Acids

We investigated a newly developed indirect competitive enzyme-linked immunosorbent assay for the determination of 5 major components of TBA, which works efficiently in different types of biological samples, and may be suitable for routine clinical practice.

scholarly journals Impact of exogenous alpha tocopherol on peanut seedlings (Arachis hypogaea L.) treated by norflurazon

2020 ◽  
Vol 63 (2) ◽  
pp. 125-133
Author(s):  
Imene Manaa ◽  
Reda Djebbar ◽  
Ouzna Abrous-Belbachir
Keyword(s):  
Arachis Hypogaea ◽  
Photosynthetic Activity ◽  
Reduced Glutathione ◽  
Soluble Sugar ◽  
Ascorbic Acid Content ◽  
Alpha Tocopherol ◽  
Glutathione S Transferase ◽  
Arachis Hypogaea L ◽  
Mda Content

Norflurazon 100 µM alone or in combination with α-tocopherol (0.25 mM) was applied in pre-emergence of peanut seedlings (Arachis hypogaea L.). Norflurazon treatment allowed to partially or totally photobleach plants which were noticeably smaller than the control. Norflurazon impaired the photosynthetic activity by decreasing photosynthetic pigments (carotenoids and chlorophylls) and by reducing quantities of soluble sugar. The determination of malondialdehyde (MDA) showed that its content was higher in treated plants in relation with enhancement of reactive oxygen species by the herbicide and decreased the endogenous α-tocopherol. The addition of exogenous α-tocopherol reduced the damage done by the herbicide at the membrane level because of the MDA content was less important than in norflurazon treated seedlings. Furthermore, the norflurazon decreased the glutathione S-transferase (GST) activity in the leaves and the roots of peanut seedlings, while it increased the level of reduced glutathione. This activity decreased even more with the application of exogenous α-tocopherol in combination with the herbicide. The herbicide alone or in association with the antioxidant α-tocopherol increased ascorbic acid content. The supplementation of α-tocopherol did not decrease the phytotoxicity of norflurazon although we observed a decrease in MDA content.

Determination of Florfenicol Amine Residues in Animal Edible Tissues by an Indirect Competitive ELISA

2008 ◽  
Vol 56 (18) ◽  
pp. 8261-8267 ◽  
Author(s):  
Jin-E Wu ◽  
Chao Chang ◽  
Wen-Ping Ding ◽  
Dong-Ping He
Keyword(s):  
Competitive Elisa ◽  
Indirect Competitive Elisa ◽  
Edible Tissues

scholarly journals Development of indirect competitive ELISA for determination of dehydroabietic acid in duck skin and comparison with the HPLC method

2020 ◽  
Vol 99 (6) ◽  
pp. 3280-3285
Author(s):  
Xinyuan Qiu ◽  
Jingjing Ma ◽  
Pengpeng Li ◽  
Zhiming Geng ◽  
Chong Sun ◽  
...  
Keyword(s):  
Dehydroabietic Acid ◽  
Hplc Method ◽  
Competitive Elisa ◽  
Indirect Competitive Elisa

Optimization and Validation of an Indirect Competitive ELISA for Detecting Enrofloxacin Residue

2012 ◽  
Vol 424-425 ◽  
pp. 603-606
Author(s):  
Shi Xiu Wu ◽  
Jin Qing Jiang
Keyword(s):  
Polyclonal Antibody ◽  
Correlation Coefficients ◽  
Competitive Elisa ◽  
Linear Range ◽  
Elisa System ◽  
Indirect Competitive Elisa ◽  
Chicken Muscle ◽  
Ic50 Values ◽  
Liver And Kidney

This article aimed to optimize a polyclonal antibody based indirect competitive ELISA (icELISA) and compare between the icELSIA method and the LC-MS technology for the determination of enrofloxacin (ENR) residue. Based on the square matrix titration, linear range of the icELISA was from 0.006 to 31.5 ng/mL, with LOD and IC50 values of 0.003 ng/mL and 0.45 ng/mL, respectively. After optimization, 0.03 mol/L of HCl was used in the assay buffer and this ELISA system can tolerate acetonitrile not higher than 10%. The recoveries of ENR were in the range of 98.3-127.5%, 85.7-112.5% and 97.4-103.8% for chicken muscle, liver and kidney. The correlation coefficients (R2) of the ELISA and LC-MS data were 0.9472 in muscle, 0.9843 in liver, and 0.9382 in kidney, respectively.

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