Determination of the total concentration of casein and β-lactoglobulin by indirect competitive ELISA in milk and dairy products

2014 ◽  
Vol 59 (27) ◽  
pp. 3385-3389
Author(s):  
Xuefei Lv ◽  
Xuena Hu ◽  
Yan Man ◽  
Yulin Deng
2003 ◽  
Vol 66 (8) ◽  
pp. 1439-1443 ◽  
Author(s):  
JEONG-HUN KANG ◽  
FUSAO KONDO

This study was conducted to develop a selective and sensitive method for the determination of bisphenol A (BPA) levels in milk and dairy products. A method based on solvent extraction with acetonitrile and solid-phase extraction (SPE) was developed for the analysis of BPA in milk, yogurt, cream, butter, pudding, condensed milk, and flavored milk, and a method using two SPE cartridges (OASIS HLB and Florisil cartridge) for skim milk was also developed. The developed methods showed good recovery levels (77 to 102%) together with low detection limits (1 μg/liter for milk, yogurt, pudding, condensed milk, flavored milk, and skim milk and 3 μg/liter for cream and butter). These methods are simple, sensitive, and suitable for the analysis of BPA in milk and dairy products. When 40 milk and dairy products were analyzed by the proposed methods, BPA was not identified in noncanned products, but its levels ranged from 21 to 43 μg/kg in canned products, levels that were 60- to 140-fold lower than the migration limits in the European Union and Japan.


1998 ◽  
Vol 46 (10) ◽  
pp. 4250-4254 ◽  
Author(s):  
María Paz Aguilar-Caballos ◽  
Agustina Gómez-Hens ◽  
Dolores Pérez-Bendito

1985 ◽  
Vol 68 (4) ◽  
pp. 693-696 ◽  
Author(s):  
Samy H Ashoor ◽  
Michael J Knox ◽  
Jacquelyn R Olsen, ◽  
Dru Ann Deger

Abstract Reported here is a simple liquid chromatographic (LC) method for the i determination of riboflavin in milk (liquid, evaporated, and dry), yogurt, ' and cheese. The method involves passing liquid samples or filtrates of semisolid and solid samples through a Clg cartridge. Retained riboflavin is then eluted with an aliquot of 50% methanol in 0.02M acetate buffer of pH 4. A volume of the eluate is injected into the LC system | consisting of a CJ8 column, a solvent of water-methanol-acetic acid (65 + 35 + 0.1, v/v) with a flow rate of 1 mL/min, and a UV detector set at 270 nm. The method is precise and accurate and compares i favorably with the present AOAC method. Moreover, it involves fewer sample preparation steps and has a total analysis time of less than 1 h.


2020 ◽  
Vol 333 ◽  
pp. 127421 ◽  
Author(s):  
Enric Pellicer-Castell ◽  
Carolina Belenguer-Sapiña ◽  
Pedro Amorós ◽  
José Manuel Herrero-Martínez ◽  
Adela R. Mauri-Aucejo

2017 ◽  
Vol 9 (4) ◽  
pp. 625-633 ◽  
Author(s):  
Shuchen Liu ◽  
Yue Zhang ◽  
Baoping Qu ◽  
Gaofeng Qin ◽  
Jinjun Cheng ◽  
...  

We investigated a newly developed indirect competitive enzyme-linked immunosorbent assay for the determination of 5 major components of TBA, which works efficiently in different types of biological samples, and may be suitable for routine clinical practice.


2013 ◽  
Vol 81 ◽  
pp. 36-41 ◽  
Author(s):  
E. Kabai ◽  
M. Beyermann ◽  
J. Seeger ◽  
B.T. Savkin ◽  
S. Stanglmaier ◽  
...  

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